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Determination of β-endorphin and substance P in small volumes of human cerebrospinal fluid (1986)

Henning, K. ; Wallasch, Th. -M. ; Przuntek, H.

Springer

Journal of neural transmission 67 (1986), S. 105-112

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ISSN: 1435-1463

Keywords: β h-endorphin ; cerebrospinal fluid ; high-performance liquid chromatography ; substance P

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary A reversed-phase high-performance liquid chromatography procedure combined with radioimmunoassay (rp-HPLC-RIA) is presented. It makes possible the quantitative determination of the neuropeptides,β-endorphin and substance P, in small volumes of cerebrospinal fluid (CSF). The concentration-dependence of the recovery of the method is demonstrated and the effect of free silanol groups of the stationary phase on the recovery is discussed. CSF was shown to contain 9.6±1.1fmolβ h-endorphin and 12.0±0.9 fmol substance P per ml.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01243363

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