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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 91 (1969), S. 2388-2389 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 23 (1974), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Synaptosomes prepared by differential and Ficoll density gradient centrifugation took up d-galactose by two saturable transport systems: one. a high affinity system with a Km of 0-25 mn and Vmax of 075 nmol/mg protein 3 min, the other, a low affinity system with a Km of 47 mM and a Vmaxof 135 nmol/mg protein/3 min. The high affinity system was inhibited by 1-5 mM phlorizin but was unaffected by the absence of sodium ion or the presence of 1 mM ouabain. The low affinity system was unaffected by phlorizin or ouabain. Both systems were inhibited by high concentrations of glucose. 2-deoxyga-lactose. and inositol, and by 2.4-dinitrophcnol. Galactose was rapidly converted in synaptosomes to phos-phorylatcd intermediates and was more slowly oxidized to 14CO2
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 26 (1976), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Influx of α-methylglucoside into synaptosomes prepared by differential and Ficoll density gradient centrifugation was studied to determine whether this sugar could be used as a model for glucose transport in nerve endings. The rate of uptake of a-methylglucoside was linear over a wide range of substrate concentrations. Influx was only slightly inhibited (12–15%) in the presence of glucose, 2-deoxyglncose, phloretin and 2,4-dinitrophenol and was unaffected by galactose or phlorizin. Conver- sion of a-methylglucoside to phosphorylated intermediates in synaptosomes was negligible. The data are consislent with the influx of a-methylglucoside being primarily a diffusion process or being mediated by a system with an extremely high Km. However, it is possible that a small portion of the sugar may be transported by the low affinity glucose transport system. The results indicate that a-methylgluco- side is not a good model for glucose transport in synaptosomes as it is in other tissues.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 31 (1978), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The uptake of inositol by rat brain synaptosomes occurs via an unsaturable process that even at substrate concentrations as low as 1 μM is unable to achieve a concentration gradient indicative of active transport. Dinitrophenol, ouabain and cytochalasin B did not affect uptake of the cyclitol. The data indicate that inositol uptake by rat synaptosomes occurs by diffusion or by a system with an affinity so low it is difficult to discern. The low capacity, saturable inositol uptake system observed in rabbit brain slices may reflect a species difference or uptake by elements of the slice other than neuronal membranes.
    Type of Medium: Electronic Resource
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