ISSN:
1432-198X
Keywords:
Diabetes mellitus
;
Isoelectric focusing
;
Urinary albumin
;
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis
;
tubular proteinuria
;
β2-Microglobulin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We studied differential urinary albumin excretion by a double one-dimensional gel electrophoresis with decyl sodium sulphate-polyacrylamide gel electrophoresis in the first, and isoelectric focusing in the second dimension in 37 diabetic children and 20 healthy subjects. In addition, total proteins, albumin, β2-microglobulin and molecular size distribution of urinary proteins were measured. the latter using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Whilst albuminuria was not significantly different from controls we found an increased microheterogeneity of urinary albumin in 38% of patients. In addition, low molecular weight protein (P〈0.05) and β2-microglobulin excretion (P〈0.01) were elevated. It is suggested that the appearance of highly heterogenous albumin in the pI range of 5.3–5.9 is the result of a decreased tubular reabsorption.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00856644
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