ISSN:
1612-1112
Keywords:
Liquid chromatography
;
Derivatization with NBD-F
;
Amino acids, biogenic amines
;
Blood analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The reaction of a newly developed fluoregenic reagent, 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole(NBD-F), with amino acids and biogenic amines was investigated. NBD-F was reactive to both primary and secondary amines including amino acids and biogenic amines such as catecholamines. The amino acids were reacted with the reagent, separated by high-performance liquid chromatography on μ-Bondapak C18 and detected at 10 to 100 fmol level. A few μg of protein hydrolysates, rabbit pyruvate kinase M1, rabbit aldolase A and papain, were adequate for the amino acids quantitation. An automatic amino acid analyzer with fluorometric detection by the post-column derivatization with NBD-F enabled the amino acid profile analysis in blood samples present in a paper disc of 3 mm diameter.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02258904
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