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  • 1
    Electronic Resource
    Electronic Resource
    The secretion and post translational modification of interferons from Saccharomyces cerevisiae (1987)
    Springer
    Current genetics 12 (1987), S. 561-567 
    Details
    ISSN: 1432-0983
    Keywords: S. cerevisiae ; Secretion ; Interferon alpha-factor ; Glycosylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Studies with three interferon molecules, IFN-α2, IFN-β 1, and a “hybrid” interferon, IFNX-430 are described which illustrate that both the expression and secretion characteristics of heterologous proteins in yeast cells reflect properties of the proteins themselves. Recombinant DNA techniques have also been used to demonstrate that the efficient processing of mature heterologous proteins from the yeast α factor secretion leader can be affected by sequences on the carboxyl side of the initial cleavage site. Secretion studies with heterologous proteins in S. cerevisiae are aimed at maximising yield, the percentage of extracefular product and correct amino terminus sequence. The results presented here show that all three factors are susceptible to currently unpredictable properties of the foreign sequence. This situation, in turn, means that heterologous proteins can be used as tools in the biochemical dissection of the yeast secretion process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368057
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The selection of promoters for the expression of heterologous genes in the yeast Saccharomyces cerevisiae (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 505-511 
    Details
    ISSN: 1617-4623
    Keywords: Yeast ; HSV TK ; Promoter ; Interferon ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A system is described that facilitates the selection of yeast promoters of unknown origin using a functional heterologous gene assay. A multicopy plasmid was constructed with a unique cloning site 5′ to the Herpes Simplex type 1 thymidine kinase gene (TK). A yeast genomic DNA library was introduced into this site and used to transform yeast (which naturally lacks a TK gene). Only transformants carrying DNA fragments with promoter activity can express TK activity and thereby grow on medium containing thymidine and folate antagonists. On the basis of TK assays of yeast cell homogenates from some 100 TK + transformants, a particularly promising plasmid containing a yeast promoter was selected for further analysis. The position of the transcriptional start of the mRNA of this promoter was identified and the promoter sequence established. A fragment containing the promoter was taken and engineered to produce an expression vector and an expression/secretion vector. We report on the expression and secretion of interferon —X430 (IFN-X 430) (a derivative of IFN beta) from yeast cells containing such plasmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331032
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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