ISSN:
1435-1463
Keywords:
Keywords: Method
;
clinico-neuropathological correlation
;
non-neuronal
;
cell culture.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary. The present study was undertaken in order to analyse the possibility of culturing post mortem derived human fibroblasts. The combination of post mortem fibroblasts with the autopsy proven and histopathologically staged brain will allow the correlative investigation of dynamic biochemical processes which are systemically underlying or accompanying a neurological and/or psychiatric disorder. These studies are limited in autopsy brain or are uncertain when the neuropathological status is lacking, i.e. when fibroblasts were obtained from living patients. Our examinations of human autopsy fibroblast and those under experimentally controlled post mortem conditions with rats clearly demonstrate that autopsy-derived fibroblasts can be reliably cultured. The cells grown displayed typical morphological and staining characteristics as well as pharmacological responsiveness. Even cells obtained from a 99 years old individual or an individual with a post mortem delay of 48 hours grew in our culture system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s007020050177
Permalink
