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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 9 (1995), S. 774-780 
    ISSN: 1432-2218
    Keywords: Hiatal hernia ; Esophageal reflux ; Anatomical reconstruction ; Laparoscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This paper presents the technique and results of an operation that restores normal anatomical and physiological antireflux mechanisms for the treatment of gastroesophageal reflux disease (GERD). The Hill procedure was modified beginning in 1973, evolving into an operation that has been standard in our practice since 1987. Major changes included total fixation of the abdominal esophagus and elimination of phrenoesophageal bundle plication. We began performing the procedure laparoscopically in 1991 and simultaneously began a study to look at our results. This is the first report of the first 44 patients operated on from October 1991 through March 1994. There was one operative complication. Mean follow-up was 14 months. One patient was lost to follow-up and one patient died. There were no long-term side effects. A Visick grading scale was designed to categorize results. Forty graded satisfactory (95%) and two unsatisfactory.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 14 (2000), S. 123-126 
    ISSN: 1432-2218
    Keywords: Key words: Laparoscopic endoluminal surgery — Gastric bleeding — Balloon-tipped cannulas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Percutaneous balloon-tipped laparoscopic cannulas designed for preperitoneal hernia repair can be readily used to treat gastric bleeding laparoscopically. Methods: Between 1995 and 1997, we successfully used balloon-tipped cannulas to visualize, biopsy, and suture acutely bleeding gastric lesions in five patients. These case histories are reviewed for this study. Results: Patients received an average of six units of blood preoperatively (range, 0–15). Operative time averaged 207 min (range, 149–270). At surgery, gastrotomies were made for cannula placement under laparoscopic visualization. Operative findings included: lesser curve gastric ulcer, Mallory-Weiss tear, prepyloric ulcer, duodenal ulcer, and angiosarcoma. Three patients had successful percutaneous suture of bleeding gastric lesions. One patient was converted to open surgery. One patient had local resection of an angiosarcoma. Conclusion: The laparoscopic use of balloon-tipped cannulas allows the expeditious diagnosis and treatment of acute gastric hemorrhage.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2218
    Keywords: Key words: Insufflation — Retroperitoneal retraction — Intervertebral fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Laparoscopic transperitoneal fusion of the L5–S1 spinal interspace has become a common procedure. Retroperitoneal retraction and laparoscopic instrumentation without insufflation also allows visualization of the upper lumbar spaces, but this procedure is much more difficult to accomplish. We review and compare our results using each of these techniques for the treatment of mechanical instability and chronic back pain. A total of 35 selected patients underwent intervertebral fusion between February 1996 and August 1998. Their mean age was 48 years. There were 22 female and 13 male patients. Standard CO2 insufflation was used in 10 patients with L5–S1 fusions. Retractional gasless technique was used in nine patients with fusions at L5–S1, 16 patients at L4–L5, one patient at L3–L4, three patients at L2–3, and one patient at L1–L2. Thus, we performed a total of 40 lumbar fusions in 35 patients. In the 19 patients with the gasless technique, a balloon dissector and retractor facilitated the retroperitoneal exposure. Seven of these 19 patients were converted to open procedures, most commonly due to lacerations of the peritoneal lining that prohibited visualization. None of the L5–S1 patients with insufflation were converted to open. Mean operative time in the insufflated patients was 152 min vs 181 min for the retractional technique. There were seven complications in the transperitoneal group: one fusion device migration, one postoperative UTI, one intracerebral hemorrhage, one severe postoperative pancreatitis, and three iliac vein lacerations. There were 16 complications in the retroperitoneal group: one deep vein thromboses, one serosal bowel injury, one small tear in the spleen, one cage migration, one postoperative pulmonary atelectasis, one postoperative hydrocele, four postoperative ileus, and six peritoneal tears. The mean postoperative stay was three days for both groups. There were no deaths. The L5–S1 interspace is best approached transperitoneally for anterior fusion. Although the retroperitoneal retractional technique is much more difficult and has a longer and steeper learning curve, it does allow laparoscopic anterior fusion of the upper lumbar spine.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1435-1803
    Keywords: Gene expression ; reporter gene assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Increased expression of the inhibitory G protein Giα-2 is assumed to contributed to desensitization of adenylyl cyclase in human heart failure. The mechanisms of upregulation involve increases in myocardial Giα-2 protein, mRNA and gene transcriptional activity. To elucidate these mechanisms in more detail, the 5′ flanking region of the human Giα-2 gene (−1214/+115 bp) was cloned upstream of the bacterial chloramphenicol acetyltransferase (CAT) gene and transfected in embryonic chick cardiomyocytes. CAT activity was measured 48 h after transfection. Unstimulated activity of the −1214/+115 bp construct was about 10fold higher than activity of the basal CAT-construct (pGEMCAT). 5′ deletion from −1214/+115 to −85/+115 bp upstream of the transcriptional start site increased, further stepwise deletions to 46/+115 gradually decreased promotor activity. Deletion from −46/+115 to −33/+115 bp completely abolished promotor activity. Stimulation of cardiomyocytes that had been transfected with the −1214/+115 CAT-construct with isoprenaline (10 μM), forskolin (10 μM), forskolin (10 μM) plus IBMX (10 μM) or dibutyryl-cAMP (1 mM) for 24 h induced an increase in CAT activity to 139±12% (n=9), 211±18% (n=12), 256±20% (n=5) and 198±28% (n=7) of unstimulated values, respectively. We conclude: 1) In chicken cardiomyocytes a sequence element of 52 bp between −85 and −33 bp is necessary to provide basal Giα-2 promotor activity. 2) Elevation of cAMP has a stimulatory effect on the human Giα-2 promotor, thereby offering a mechanism for β-adrenoceptor-mediated increases in Giα-2 in the heart.
    Type of Medium: Electronic Resource
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