ISSN:
1573-0972
Keywords:
Electroporation
;
Streptococcus bovis
;
rumen
;
plasmid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract An improved method for electroporation of the ruminal bacterium Streptococcus bovis was developed. The organism was grown aerobically in hyperosmotic medium in the presence of 0.5% (w/v) glycine, and electroporation was carried out in a sucrose-glycerol solution with a field strength of 12.5kV/cm, 200Ω resistance and 25μF capacitance. Electroporation efficiencies of 0.5 to 2.0 × 105 transformants/μg DNA were achieved. Improved vectors for S. bovis were developed that include a multiple cloning site, and also a promoter region from the S. bovis intracellular amylase gene that may serve as an expression system for foreign genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018522721626
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