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  • 1
    Electronic Resource
    Electronic Resource
    The Genome-linked Protein (VPg) of Southern Bean Mosaic Virus is Encoded by the ORF2 (1998)
    Springer
    Virus genes 17 (1998), S. 21-24 
    Details
    ISSN: 1572-994X
    Keywords: sobemovirus ; luteovirus ; genome organization ; protein microsequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The sequence of the 20 N-terminal amino acids of the viral protein (VPg) which is covalently attached to the genomic RNA of the bean strain of Southern bean mosaic virus (SBMV-B) has been determined. The obtained VPg sequence mapped to position 327 to 346 of the SBMV-B ORF2 product, downstream of the putative protease domain and in front of the RNA-dependent RNA polymerase. Thus indicating that the sobemovirus genomic arrangement is similar to that of subgroup II luteoviruses. Comparison with other viral sequences revealed a high similarity with the sequence of the ORF2-product of the cowpea strain of SBMV (SBMV-C). No significant similarities were detected with amino acid sequences derived of other sobemoviruses or non-related viruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008044715899
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of tobacco rattle virus in nematodes by reverse transcription and polymerase chain reaction (1994)
    Springer
    European journal of plant pathology 100 (1994), S. 109-122 
    Details
    ISSN: 1573-8469
    Keywords: Trichodrodae ; Petunia hybrida ; Chenopodium amaranticolor ; Trichodorus primitivus ; Nicotiana tabacum cv. ; White Burley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An assay, based on amplification of cDNA synthesized from genomic viral RNA, has been developed to detect tobacco rattle virus in infected plant material and viruliferous nematodes. A range of different TRV strains could be detected using the procedure developed. The presence of one to three viruliferous nematodes in a nematode suspension was sufficient for the detection of TRV. The minimum amount of purified virus detectable in the assay was 15 fg, indicating an increased sensitivity of the PCR-based assay as compared to serological detection methods, like ELISA. A dot-blot hybridization procedure was developed for the detection of the PCR products, making agarose gel electrophoresis dispensable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01876245
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular breeding for virus resistant potato plants (1992)
    Springer
    European journal of plant pathology 98 (1992), S. 29-36 
    Details
    ISSN: 1573-8469
    Keywords: genetic engineering ; resistance genes ; transgenic virus resistance ; viral genes ; PVX ; PVY ; PLRV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To engineer resistance against potato virus X (PVX), the viral coat protein (CP) gene has been introduced into two potato cultivars. Stable expression of the gene in transgenic clones throughout the growing season has been obtained and resulted in considerably increased virus resistance. With varying frequencies depending on the original cultivar used, true-to-type PVX resistant transgenic clones have been obtained. Since deviant light sprout characteristics were invariably associated with aberrations in plant phenotype, they can be used in procedures to early screen for deviations. Furthermore, it has been possible to unequivocally discriminate between the original untransformed and independent transgenic cultivars. Although no relation has been found between the presence, if any, of the CP of potato virus Y (PVY) or potato leafroll virus (PLRV) in CP gene transgenic potato, appreciable levels of resistance to these viruses has been obtained. This suggests that the mechanism by which a viral CP gene in the potato genome evokes resistance, differs amongst various viruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974469
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection (1991)
    Springer
    Plant molecular biology 17 (1991), S. 431-439 
    Details
    ISSN: 1573-5028
    Keywords: potato ; potato leafroll virus ; PLRV ; virus resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic potato plants, cultivar Désirée, were produced that contained the coat protein gene of potato leafroll luteovirus (PLRV). The transformed potato plants expressed the PLRV coat protein (CP) RNA sequences but accumulation of coat protein in transgenic tissues could not be detected. Upon inoculation with PLRV, the PLRV CP RNA expressing potato plants showed a reduced rate of virus multiplication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040637
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    Paper (German National Licenses)
    Fulltext
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