ISSN:
0003-276X
Schlagwort(e):
Life and Medical Sciences
;
Cell & Developmental Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Medizin
Notizen:
Adjuvant-induced penetration of horseradish peroxidase (HRP) into seminiferous epithelium was studied as a model of adjuvant-induced entry of other proteins, e.g., immunoglobulins. HRP intravenously injected 20 minutes prior to sacrifice (by peraortic perfusion with Karnovsky's fixative) into male guinea pigs that had received Freund's complete adjuvant (FCA) + saline or FCA + testicular homogenate (TH) intradermally seven days previously, entered the peritubular spaces of a moderate number of seminiferous tubules. In those tubules HRP became uniformly distributed in the cytoplasmic matrices (but very rarely in membrane-bound vesicles) of some of the Sertoli cells and presumptive type B spermatogonia. By contrast, HRP injected into normal animals, into animals that had received FCA + saline or FCA + TH 3 or 14 days previously or into animals that had received Freund's incomplete adjuvant + saline 3, 7, or 14 days previously gained entry into only occasional peritubular spaces but was never found in any cells of the seminiferous epithelium.These findings are discussed in conjunction with previous findings on immune aspermatogenesis. Hypotheses concerning the intratesticular mechanisms of production of both active and passive immune aspermatogenesis as activated by FCA and the mechanisms whereby FCA may enable or potentiate production of immune globulins are proposed and discussed.
Zusätzliches Material:
14 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/ar.1091760106
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