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Measurements of oxygenation and perfusion in skeletal muscle using multiple microelectrodes (1997)

Greenbaum, A. R. ; Etherington, P. J. E. ; Manek, S. ; [et al.]

Springer

Journal of muscle research and cell motility 18 (1997), S. 149-159

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ISSN: 1573-2657

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract This paper describes an apparatus to measure tissue oxygenation and perfusion (as measured by the wash-in rate of gaseous hydrogen) simultaneously at multiple points in muscle using needle microelectrodes. The development of suitable electrodes and apparatus is described, as well as the development of the method and its validation. In particular, the potential for tissue damage secondary to electrode insertion, the need for in vivo voltammetric determination of the operating potential and the extent of any electrode-tissue and of electrode--electrode interactions are explored, and are shown to be insufficient in magnitude to affect the technique. Its subsequent use to characterise oxygenation and perfusion in rabbit skeletal muscle at rest is also described. In resting tibialis anterior muscle of the rabbit the mean pO2 was 18 ± 13.3 mm Hg and the mean perfusion was 4.4 ± 1.3 ml s−1 100 g−1. There was a heterogeneity in simultaneously-measured values of pO2 and perfusion at different points within muscle, and also a temporal variation at the same site. The spans between the highest and lowest simultaneously-measured values of pO2 in muscle ranged from 14 to 80 mm Hg, and for perfusion, from 1 to 12 ml s−1 g−1. No significant correlation was evident from histological examination between either pO2 or perfusion and surrounding fibre type or capillary density

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1018653521686

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Influence of solvent composition on the mechanical properties of arterial elastin (1990)

Winlove, C. P. ; Parker, K. H.

New York : Wiley-Blackwell

Biopolymers 29 (1990), S. 729-735

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ISSN: 0006-3525

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: We have investigated the effects of changes in solution composition on the mechanical properties of rings of arterial elastin. The time course of force equilibration at constant strain following a change in the composition of the bathing solution was measured. Both the force developed during slow extension and force relaxation following rapid straining were also measured in each of the test solutions. The results are difficult to summarize because all of the primitive quantities measured - sample dimension, slope of the force-extension curve, force overshoot and time of relaxation - as well as the derived quantities such as elastic modulus changed in different and apparently uncorrelated ways. Changes in pH and ionic composition of the bathing solution had small effects consistent with the low fixed charge density of elastin. Solutions of glucose, sucrose, and ethylene glycol had larger effects consistent with changes in hydrophobic interactions. The viscosity of the solution that penetrated the intrafibrillar space of the elastin appeared to be a major determinant of the dynamic response.

Additional Material: 2 Ill.