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  • 1
    Electronic Resource
    Electronic Resource
    Evaluation of anther-derived Streptocarpus X hybridus and their progeny (1986)
    Springer
    Plant cell, tissue and organ culture 6 (1986), S. 167-172 
    Details
    ISSN: 1573-5044
    Keywords: cape primrose ; Gesneriaceae ; anther culture ; somaclonal variation ; caulogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regeneration of plants by caulogenesis of anther derived callus of Streptocarpus X hybridus ‘Concorde’ was obtained on MS basal medium with 2.0 mg/l NAA and 0.5 mg/l BAP. All regenerated plants were diploid and of somatic cell origin as determined by chromosome counts, flower color and morphological similarity to the anther donor plant, and flower color segregation among progeny of anther-derived plants. Variation expressed as deformed and dwarfed transient morphology was observed in 2 anther-derived plants. Variation was also observed among progenies of 5 anther-derived plants from the same anther donor plant for days to flowering, flowers per peduncle, peduncle length, and leaf area. Additional morphological variation including transient leaf variegation among seedlings and twisted or inverted leaves on mature plants was also observed among progeny of anther-derived plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180800
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An optimized protocol for rapid and sensitive application of southern (Northern) hybridization by using fluorescein labeling and detecting system (1999)
    Springer
    Biotechnology techniques 13 (1999), S. 431-435 
    Details
    ISSN: 1573-6784
    Keywords: fluorescein labeling and detecting ; melon (Cucumis melo L.) ; Southern/Northern hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An optimized complete protocol that produces consistent Southern hybridization results (RFLP) for as many as 240 samples in 36 h is presented. Signals can be detected from 0.1 μg genomic DNA. The protocol can be adapted to Northern hybridization by using 0.05 M NaOH as transfer buffer in a downward blotting method. Probes can be removed in stripping solution containing 2–5 mM EDTA. The protocol using this system has many advantages over the conventional radioactive method as safety, rapidness, sensitivity, and signal quality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008916402379
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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