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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 387 (1997), S. 358-359 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] It has been assumed for more than 30 years that in chimpanzee communities the observed social unit and the reproductive unit are identical. We present genetic evidence that female chimpanzees seek to mate outside their own social group. We have studied a community of West African chimpanzees in ...
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0032-8332
    Keywords: Pan ; Mitochondrial DNA ; Microsatellites ; D-loop ; Cytochrome b
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic studies of free-ranging primates have been seriously impeded by difficulties of sampling tissues, including the undesirability of bleeding habituated animals, of transporting frozen samples to the laboratory, and of the inherent inadequacies of accessible variation including allozymes, mtDNA RFLP patterns and DNA fingerprints. We have developed methods of non-invasive DNA sampling and DNA-level genotyping which, when combined with a hierarchical analysis of mtDNA sequences and hypervariable nDNA simple sequence repeat (microsatellite) loci size length polymorphisms, facilitate the resolution of most questions at the individual, social group (community), population, and species (phylogenetic) levels. This approach, based on DNA amplified from shed hair, represents an important new tool for the acquisition of genetic information and will facilitate the study and management of both captive and free-ranging chimpanzees (Pan troglodytes). Our hierarchical analysis of population genetics of chimpanzees has revealed high historical levels of gene flow and large effective population sizes, as well as substantial divergence between the West African subspecies and chimpanzees from central and East Africa. At the community level, closer relatedness among philopatric males than among females supports the view that kin selection has been an evolutionary force shaping male-male cooperation in this species. Results from our study of the now relatively isolated Gombe community suggest that habitat fragmentation affects population genetic structure and possibly population viability.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Primates 34 (1993), S. 333-346 
    ISSN: 0032-8332
    Keywords: Genetics ; Pedigrees ; Molecular evolution ; Pan ; Hylobates ; Macaca ; DNA sequences ; Microsatellite loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using DNA amplified from shed or plucked hair follicles it is now possible to genotype individual primates at many nuclear and mitochondrial gene loci. Sequence specific primers and the polymerase chain reaction permit the rapid production of sufficient DNA from a single hair for numerous analyses. The direct sequencing of relatively conservative mtDNA sequences like cytochromeb is proving useful in establishing species and subspecies-level relationships. More variable sequences (e.g. the mtDNA control region or D-loop) are useful at the population and social community levels. Paternity exclusion, pedigree relationships, and community structure can be determined using simple sequence length polymorphisms (SSLPs) of multiple hypervariable nuclear microsatellite or simple sequence repeat (SSR) loci. Studies involving captive and free-ranging chimpanzees, gibbons, and macaques illustrate the resolving power of these new non-invasive molecular genetic genotyping techniques.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4927
    Keywords: Biomphalaria glabrata ; Schistosoma mansoni ; enzymes ; susceptibility to infection ; linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The snail, Biomphalaria glabrata, is a major intermediate host of the human blood fluke, Schistosoma mansoni, in the Americas. The inheritance and linkage relationships of a gene enabling adult snails to resist infection by a Puerto Rican strain of the parasite were analyzed using two laboratory stocks that differed in susceptibility, pigmentation, and five electrophoretically detectable enzyme markers. Segregation ratios in second-generation intraspecific hybrids between susceptible (M-stock) and resistant (10-R2-stock) snails indicate that the susceptibility gene is not linked to the enzyme (ACON-1, ACP, EST-2, PEP-2, PGD) or pigmentation loci studied. These seven loci assort independently of one another. Observed rates of infection among F1 and F2 progeny are consistent with Richards' finding that adult susceptibility to the PR-1 strain of S. mansoni is controlled by a single locus with resistance dominant. No association between allozymes of acid phosphatase and snail susceptibility to PR-1 was seen in the snail-parasite combinations studied.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-8604
    Keywords: microsatellites ; common marmosets ; mating system ; paternity ; relatedness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We established pedigree relations in three wild common marmoset social groups for which observational data were available, together with genotypes of some individuals from neighboring groups. Relatedness of 40 individuals were based on 11 microsatellite loci amplified from nDNA obtained noninvasively from plucked hair. The wild marmosets were only half as variable as a captive population characterized previously: 2–6 alleles/locus; HO = 0.41 and HE = 0.35. Parentage exclusion probabilities were 61.8% for an offspring and one alleged parent and 90.7% for an offspring with one confirmed and one alleged parent. Each group (n = 5–14 individuals) had two breeding females and ≥2 adult males. Within each group the infants and reproductively inactive adults were closely related to at least the breeding females; the latter were related to each other as closely as mother/infant pairs or sisters. Relatedness of adult males was lower, indicating recent intergroup dispersal. Genetic data confirm Callithrix jacchus live in relatively stable extended family groups of closely related individuals. Matings occurred preferentially among the least related adults and most infants were fathered by the dominant male. The genetic data are consistent with polygynmonandry as are the field observations. Callithrix have variable mating systems, ranging from monogamy to polyandry to polygyny within social groups plus extragroup copulations; our data provide no evidence for polyandry and are inconclusive with respect to extragroup paternity. Nevertheless, noninvasive multilocus genotyping methods will resolve these questions when longer-term studies of entire populations are undertaken.
    Type of Medium: Electronic Resource
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