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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 75 (1990), S. 351-358 
    ISSN: 1573-2622
    Keywords: Nylon ; stainless steel ; free surface energy ; fibroblast cells ; collagen network ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Nylon and stainless steel sutures separately placed deeply into rabbit corneas by splitting the stroma for a few millimeters, without closing sutures, remained in the cornea for two, four and six weeks respectively. In contrast to the stainless steel sutures an extensive tissue reaction could be observed clinically around the nylon sutures within a few days and was still present after 4–6 weeks. On the endothelial side, covering of the nylon with fibroblast cells took place very slowly, this in contrast with stainless steel. Irritation of the tissue and an oedematous appearance of the endothelium around the nylon suture was the result. At first a kind of collagen network, often mixed with inflammatory cells, was deposited on the nylon material before fibroblast cells could grow in. Covering of the stainless steel started quite soon after implantation, without preliminary deposition of collagen material. The reason for this phenomenon must be sought in the high free surface energy of the stainless steel, which attracts cells, in contrast to nylon which has a very low free surface energy.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 75 (1990), S. 335-341 
    ISSN: 1573-2622
    Keywords: Concave intraocular lens ; myopia ; anterior chamber
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Surgical correction of high myopia is indicated in a limited number of cases. Techniques include (epi)keratophakia, keratomileusis and implantation of a negative-power IOL. The iris claw lens, fixed to the midperiphery of the iris, has proved its safety in aphakic eyes. The model has been adapted by giving it a vault in order to make it suitable for implantation in high myopic phakic eyes. The implantation technique and short-term results of patients operated by Worst are presented.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 75 (1990), S. 365-375 
    ISSN: 1573-2622
    Keywords: Glaucoma surgery ; stainless steel microspiral ; ab interno procedure ; gonioseton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A new and simple technique for glaucoma filtering surgery is presented. It is based on the introduction of a stainless steel microspiral or gonioseton into a goniopuncture by way of an ab interno procedure, minimising surgical trauma to the filtration area. Preliminary results are presented.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 75 (1990), S. 377-390 
    ISSN: 1573-2622
    Keywords: Intracapsular cataract extraction (ICCE) ; ‘single-handed’ freon cryo-extractor ; adjustable Flieringa ring ; Iris (Lobster) Claw Lens ; stainless steel sutures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A description of the author's (J.W.) technique for ICCE using a ‘single-handed’ freon cryo-extractor is given. Flieringa's ring is used for scleral support. Implantation is performed with an Iris Claw Lens. Stainless steel is used as suturing material.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 78 (1991), S. 317-324 
    ISSN: 1573-2622
    Keywords: Anterior capsule ; degeneration ; non-coating technique ; capsular epithelium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Lens capsules of patients of advanced age, obtained after extracapsular cataract surgery, were carefully prepared for a combined LM, TEM and SEM investigation, after preliminary washing and mounting onto a holder in a buffer solution. After pre-fixation with GA, samples were postfixed for LM/TEM and OsO4/K4Fe(CN)6 and stained with toluidineblue/basic fuchsine for LM and with uranyl acetate/lead citrate for TEM; for SEM the GA-pre-fixed samples were post-fixed by the Tannine Arginine-OsO4 non-coating technique. At LM-level discrimination between healthy and degenerating cells was possible after toluidine staining. At SEM-level protrusion of the cell nucleus and fibrillation and blebbing of the cell membrane as the result of capsular degeneration could be observed with the TAO-method. At TEM-level protrusion of the cell nucleus, degeneration of the cytoplasm, ballooning of the mitochondria, the presence of microfilaments and the occurrence of vacuoles were visible as the result of capsular degeneration on cataract formation.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 82 (1992), S. 125-133 
    ISSN: 1573-2622
    Keywords: (An)aerobic bacteria ; ECCE ; Golfball structure ; Micro-organisms ; Quiet eyes ; Secondary-cataract ; SEM/TEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Twentyfour patients, who had marked reduction of vision due to secondary-cataract developed after an ECCE, were treated by surgical cleaning of the posterior lens capsule. During this procedure globular secondary-cataract material was removed and collected for morphological examination by SEM and TEM. Fragments of various sizes and shapes, including some with a ‘golfball’ structure, were seen; these closely resembled particles frequently found in cataractous lenses. In addition, in 18 patients micro-organisms were found: rod-shaped bacteria, cocci, and in 2 cases yeasts. These findings were the more remarkable because these were clinically quiet eyes with no signs of intra-ocular inflammation and cultures have been persistently negative. We imagine that these bacteria must have entered the eye during the cataract extraction and have settled there without causing an infection.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 82 (1992), S. 169-178 
    ISSN: 1573-2622
    Keywords: Bursa premacularis ; Cisterns ; Human ; Ink-injection technique ; Rhesus monkey (Macaco mulatta) ; Slitlamp microscopy ; Tracts ; Vitreous body
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the isolated unfixed vitreous body a structural organization can be visualized by slitlamp microscopy or by an ink-injection technique. We discuss the observations on human and rhesus monkey (Macaca mulatta) vitreous bodies using the ink-injection technique. Advantages and disadvantages of this method compared with slitlamp microscopy are described. Eventual functional implications of vitreal organization are mentioned briefly.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 85 (1993), S. 67-75 
    ISSN: 1573-2622
    Keywords: Human anterior lenscapsule ; Cataractogenesis ; LM ; SEM ; TEM ; Capsule epithelial cell degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The degeneration of the capsule epithelium of cataractous lenses has been studied with LM, SEM en TEM with emphases on TEM. The observed degeneration of the epithelial cells can be described as follows: The cell nucleus becomes picnotic and desintegrates as result of change of the chromatin. Degeneration of the cytoplasm starts with swelling of the mitochondria, coming into existence of filamentous networks and balloon-like bulges of the nucleus. Repelling of the cell nucleus due to a porosity of the plasm membrane. Collapse of the cell due to degeneration of the cell and loss of the cytoplasmic contents, leaving finally only a swollen framework of cell walls.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 85 (1993), S. 77-85 
    ISSN: 1573-2622
    Keywords: Cataractous lensfibres ; Spherical structures ; Vacuoles ; Degeneration ; Cyto-pathology ; Electronmicroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The degeneration process of lensfibres in a cataractous lens, described as the biochemical changes of a part of the lensproteins, can be characterised morphologically as follows: Emulsification of a part of the lensfibre-mass and the development of open spaces between the lensfibres with the formation of vacuoles (spherical structures) with a granular or a compact contents with high contrast, which is squeezed into the intercellular space. Degeneration of the ball & socket system interdigitation and microvilli domain system and the formation of almost empty rectangular structures in somewhat radial array. Balloon-like bulging of the cytoplasm of the lenscell, degeneration of the cytoplasm membrane and visualisation of a micro-filamentous network with enclosed cell organelles.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 70 (1988), S. 155-163 
    ISSN: 1573-2622
    Keywords: granular/fibrillar lens proteins ; recrystallization of lens protein ; senile cataract ; water-clefts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two lenses from patients of very advanced age with senile cataracts were processed for SEM, fractured equatorially, sputtered with Au and examined by SEM. In the cross-fracture various areas could be observed. Although the overall structure of the lens-fibres appeared to be intact, higher magnifications showed that the len-fibre material had changed into a brittle structure, with either a granular appearance or a fibrillar character. At other places clearly recrystallization of lens-fibre proteins had taken place, with the formation of finger-like substructures, sometimes organized into plate-like structures or running parallel to each other in a kind of undulating pattern. Between the various areas of chemically changed lens-fibre material certain ‘canal-like’ areas were found with cellular structures, the so-called ‘waterclefts’ or ‘Wasserspalten’. Structures which, together with the chemical change in the lens proteins, account for the dramatic change in light dispersion.
    Type of Medium: Electronic Resource
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