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  • 1
    Electronic Resource
    Electronic Resource
    Induced ADH gene expression and enzyme activity in pollinated pistils of Solanum tuberosum (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 107-109 
    Details
    ISSN: 1432-2145
    Keywords: Key words Pistil ; Pollen tube growth ; Pollen ; Alcohol dehydrogenase ; ADH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The regulation of alcohol dehydrogenase (ADH) in relation to in vivo pollen tube growth of Solanum tuberosum was investigated. Adh gene expression as well as ADH enzyme activity were induced in pollinated pistils. The induced ADH isozyme in pollinated pistils is not present in pollen or anthers. The same ADH isozyme is induced in leaves submerged in water. The significance of the induction of ADH activity for pollen tube growth is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050075
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The promoter of a gene that is expressed only in pollen interacts with ubiquitous transcription factors (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 197-202 
    Details
    ISSN: 1432-2145
    Keywords: Tobacco ; Pollen ; Transcriptional control ; GT-1 · GT-2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheNTP303 gene of tobacco is expressed only in bicellular pollen. The minimal pollen-specific promoter of the gene is located between −103 and −51 upstream of the transcriptional start site. Using gel mobility shift assays, we demonstrated that this minimal functional promoter interacts with a leaf nuclear GT-1 binding activity. This finding suggests that ubiquitous transcription factors are involved in the pollen-specific expression of the gene in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173098
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mass-spectrometric quantitation of cytokinins in tobacco crown-gall tumours induced by mutated octopine Ti plasmids of Agrobacterium tumefaciens (1988)
    Springer
    Planta 176 (1988), S. 230-234 
    Details
    ISSN: 1432-2048
    Keywords: Agrobacterium ; Auxin in tumors ; Cytokinin (in tumors, turnover, oxidase) ; Mutant (T-DNA) ; Nicotiana (crown gall) ; T-DNA mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The levels of the major cytokinins, zeatin, zeatin riboside, zeatin riboside-5′-monophosphate and zeatin-7-glucoside were measured in tobacco (Nicotiana tabacum L.) crown-gall tissues carrying insertion and deletion mutations in the T-DNA. Measurements were made by combined gas chromatography-mass spectrometry using selected ion monitoring with 15N- and 2H-labelled internal standards. The results demonstrate that, relative to wild-type tumour tissue, cytokinin levels are considerably elevated in tissues lacking functional T-DNA auxin-biosynthetic genes. From a detailed analysis of the major cytokinin metabolites it is concluded that a reduction in the extent of cytokinin degradation via N6-side-chain cleavage is an important factor leading to increased cytokinin levels in these tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392449
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  • 4
    Electronic Resource
    Electronic Resource
    Visualization of starch-synthase expression by in situ hybridization during pollen development (1991)
    Springer
    Planta 184 (1991), S. 182-186 
    Details
    ISSN: 1432-2048
    Keywords: Gene expression ; Hybridization in situ ; Nicotiana ; Pollen development ; Starch synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression of the starch-synthase gene was studied by in situ RNA hybridization at different stages of pollen development in tobacco (Nicotiana tabacum L.). By means of Northern blot analysis we demonstrated that a potato (Solarium tuberosum L.) gene was homologous to its tobacco counterpart, and then we synthesized 35S-labeled RNA probes from this gene. The probe was seen to bind at the tetrad stage. Unicellular pollen grains showed no starch-synthase mRNA but these transcripts reappeared in the binucleate stages, being abundant in mature pollen grains. Our results show that starch-synthase is expressed in the gametophytic cells. Variations in mRNA accumulation during the different stages indicate a regulation of this gene throughout pollen development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197946
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  • 5
    Electronic Resource
    Electronic Resource
    Visualization of starch-synthase expression by in situ hybridization during pollen development (1991)
    Springer
    Planta 184 (1991), S. 182-186 
    Details
    ISSN: 1432-2048
    Keywords: Gene expression ; Hybridization in situ ; Nicotiana ; Pollen development ; Starch synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression of the starch-synthase gene was studied by in situ RNA hybridization at different stages of pollen development in tobacco (Nicotiana tabacum L.). By means of Northern blot analysis we demonstrated that a potato (Solarium tuberosum L.) gene was homologous to its tobacco counterpart, and then we synthesized35S-labeled RNA probes from this gene. The probe was seen to bind at the tetrad stage. Unicellular pollen grains showed no starch-synthase mRNA but these transcripts reappeared in the binucleate stages, being abundant in mature pollen grains. Our results show that starch-synthase is expressed in the gametophytic cells. Variations in mRNA accumulation during the different stages indicate a regulation of this gene throughout pollen development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01102417
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation and characterization of mRNAs accumulated during in-vitro flower bud formation (1994)
    Springer
    Planta 195 (1994), S. 271-281 
    Details
    ISSN: 1432-2048
    Keywords: cDNA (flowering) ; Cell culture (thin layers) ; Gene expression ; Flower bud initiation ; mRNA (flowering) ; Nicotiana (flower bud formation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of vegetative and generative buds on thin-layer expiants of tobacco (Nicotiana tabacum L. cv. Samsun) has been studied at the level of translatable mRNA to detect changes in the mRNA population during bud initiation and differentiation, and several quantitative differences were found. By differential screening of a cDNA library obtained from flower-bud-regenerating explants we have isolated a group of six cDNA clones representing genes that are preferentially expressed during in-vitro flower bud formation. Nucleotide sequence analysis of one of these cDNAs, pAP8, showed that the most likely open reading frame has some typical characteristics of, and homology with, extensin-like genes. Northern blot analysis and in-situ hybridization suggest a specific role for these extensin-like genes in flower bud initiation on tobacco pedicel explants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199687
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  • 7
    Electronic Resource
    Electronic Resource
    The pollen-specific gene Ntp303 encodes a 69-kDa glycoprotein associated with the vegetative membranes and the cell wall (2000)
    Springer
    Sexual plant reproduction 12 (2000), S. 276-284 
    Details
    ISSN: 1432-2145
    Keywords: Key words NTP303 glycoprotein ; Pollen-specific Ntp303 gene ; Pollen-tube specific protein ; Plant reproduction ; Biochemical analysis ; Pollen development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The pollen-specific gene Ntp303 belongs to the class of late pollen specific genes. It is first transcribed directly after pollen mitosis. Biochemical properties, appearance and precise location of the NTP303 protein during pollen development and pollen tube growth were studied by amino-acid micro-sequencing, protein gel blotting and immuno-localization. Antisera were raised against recombinant proteins, encoded by sequences of the pollen-specific Ntp303 gene. The antibodies specifically recognized a 69-kDa glycoprotein. Electron-microscopic immuno-localization of the protein revealed the presence of high concentrations of the NTP303 protein at the vegetative plasma membranes that surround the vegetative cell, the generative cell and the sperm cells of pollen and pollen tubes. The generative plasma membranes of the generative cell and the sperm cells were negative. NTP303 protein was also present in the cell walls and in callose plugs. With this method it was shown that the NTP303 protein was already present in mid-bicellular pollen, after the first, asymmetrical pollen mitosis. Possible functions for the NTP303 protein are discussed in relation to its properties and its association with the vegetative plasma membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050195
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  • 8
    Electronic Resource
    Electronic Resource
    The promoter of a gene that is expressed only in pollen interacts with ubiquitous transcription factors (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 197-202 
    Details
    ISSN: 1432-2145
    Keywords: Key words Tobacco ; Pollen ; Transcriptional control ; GT-1 ; GT-2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The NTP303 gene of tobacco is expressed only in bicellular pollen. The minimal pollen-specific promoter of the gene is located between –103 and –51 upstream of the transcriptional start site. Using gel mobility shift assays, we demonstrated that this minimal functional promoter interacts with a leaf nuclear GT-1 binding activity. This finding suggests that ubiquitous transcription factors are involved in the pollen-specific expression of the gene in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050031
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Characterization of a pollen-specific cDNA clone from Nicotiana tabacum expressed during microgametogenesis and germination (1992)
    Springer
    Plant molecular biology 18 (1992), S. 1101-1111 
    Details
    ISSN: 1573-5028
    Keywords: Nicotiana tabacum ; pollen-specific cDNA ; (pollen) germination ; pollen tube growth ; microgametogenesis ; pollen-specific expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This report describes the isolation and characterization of a cDNA clone representing a gene specifically expressed in pollen. A cDNA library was constructed against mRNA from mature pollen of Nicotiana tabacum. It was screened differentially against cDNA from mRNA of leaf and of pollen. One clone, NTPc303, was further characterized. On northern blot this clone hybridizes to a transcript 2100 nucleotides in length. NTPc303 is abundant in pollen. Expression of the corresponding gene is restricted to pollen, because no other generative or vegetative tissue contains transcripts hybridizing to NTPc303. Expression of NTP303 is evolutionarily conserved: homologous transcripts are present in pollen from various plant species. The first NTP303 transcripts are detectable on northern blot at the early bi-nucleate stage and accumulate until the pollen has reached maturity. During germination and pollen tube growth in vitro new NTP303 transcripts appear. This transcription has been proved by northern blots as well as by pulse labelling experiments. Nucleotide sequence analysis revealed that NTPc303 has an open reading frame coding for a predicted protein of 62 kDa. This protein shares homology to ascorbate oxidase and other members of the blue copper oxidase family. A possible function for this clone during pollen germination is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00047713
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Non-oncogenic T-region mutants ofAgrobacterium tumefaciens do transfer, T-DNA into plant cells (1983)
    Springer
    Plant molecular biology 2 (1983), S. 155-163 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; site-directed mutagenesis ; non-oncogenic T-region mutants ; T-DNA transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new procedure for site-directed mutagenesis has been applied to the shooting and rooting loci of T-DNA of an octopine Ti-plasmid ofAgrobacterium tumefaciens. Mutants have been obtained which induced tumours that either developed shoots or produced more roots than normally observed. Double mutations, in which both types of T-DNA loci were affected, resulted in non-oncogenic strains. Indications have been obtained, showing that T-DNA coded oncogenic functions can be eliminated without affecting T-DNA transfer into plant cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01578515
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    Paper (German National Licenses)
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