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  • 1
    Electronic Resource
    Electronic Resource
    Subcellular Site of Action of Imipramine in Rodent Brain (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 46 (1986), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: To determine the site of action of imipramine, the subcellular distribution of [3H]imipramine in rodents was followed after both in vivo administration and in vitro incubation with tissue slices under “physiological” conditions. Total [3H]imipramine (10–1,000 nM) binding was associated with all primary fractions, but in particular with the nuclear (P1) and mitochondrial (P2) pellets and the synaptosomal (P2B) and myelin (P2A) fractions; Using an excess of imipramine to define any nonspecific interactions, a specific association was observed mainly in those fractions containing isolated nerve terminals and to a lesser extent with the purified myelin fraction. Preparation of subsynaptosomal fractions by osmotic lysis indicated that [3H]imipramine was associated with the synaptic vesicle and microsomal fractions and also with synaptosomal membranes. The degree of binding to the vesicular and microsomal fractions was increased with the length of preparation time, whereas there was an inverse relationship between the length of preparation and the amount bound to the synaptosomal membrane fraction. There was no evidence of an intrasynaptosomal accumulation of [3H]imipramine at concentrations up to 1,000 nM. [3H]2-Nitroimipramine, a slowly dissociating imipramine derivative, was exclusively located in synaptic membrane fractions. Prior treatment of rats with a combination of 5,7-dihydroxytryptamine and desipramine reduced 5-hydroxytryptamine levels and the levels of [3H]imipramine associated with the synaptosomal fractions to the same extent. It is concluded that imipramine is associated with a binding site localised on 5-hydroxytryptaminergic nerve terminals and that there is a redistribution to other sites (vesicular and microsomal) during the isolation procedure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1986.tb00611.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of histamine antagonists on noradrenaline-stimulated blood flow and oxygen consumption of brown adipose tissue in the rat (1984)
    Springer
    Pflügers Archiv 402 (1984), S. 325-329 
    Details
    ISSN: 1432-2013
    Keywords: Brown adipose tissue ; Noradrenaline ; Thermogenesis ; Histamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract 1. The effects of histamine antagonists on noradrenaline-stimulated metabolic rate, tissue blood flow (estimated from the distribution of radiolabelled microspheres) and brown adipose tissue (BAT) oxygen extraction were studied in male anaesthetised rats. 2. Injection of cimetidine (H2-receptor antagonist), reduced the noradrenaline-stimulation of metabolic rate and the increase in blood flow to BAT, but did not affect blood flow to white adipose tissue, skin, leg muscle, kidney, brain, or testes. 3. Following noradrenaline, in vivo oxygen consumption of BAT, estimated from blood flow and oxygen extraction was depressed to 31% of control values by treatment with cimetidine, blood pressure was unaffected. 4. Injection of the histamine (H1-receptor) antagonist, mepyramine, did not affect tissue blood flow or metabolic rate. 5. In conscious animals, cimetidine did not affect resting oxygen consumption, but depressed the thermogenic responses to noradrenaline. 6. These data suggest that the stimulation of BAT blood flow and thermogenesis by noradrenaline may be mediated in part by histamine acting on an H2-type receptor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585518
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  • 3
    Electronic Resource
    Electronic Resource
    Critical assessment of noradrenaline uptake in synaptosomal preparations (1983)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 322 (1983), S. 129-135 
    Details
    ISSN: 1432-1912
    Keywords: Noradrenaline uptake ; Synaptosomes ; Vertical rotor ; LiCl correction ; Tricyclic antidepressant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Synaptosomes and other subcellular organelles were prepared from rat brain using a vertical rotor. The preparation time was reduced by up to 60% compared to conventional techniques. Uptake of [3H]-(−)-noradrenaline into subcellular fractions was characterised. The characteristics of this uptake were dependent on the subcellular composition and anatomical origin. Various methods of correction for energy independent processes were compared, but only sodium ion removal from the medium selectively inhibited the energy dependent uptake mechanism. Kinetic analysis of data revealed that high and low affinity uptake systems existed. The relative contributions of these systems were dependent on the fraction under analysis. Noradrenaline uptake was not exclusively localised in noradrenergic terminals. Selective inhibitors of the noradrenaline uptake process (tricyclic antidepressants) inhibited energy dependent uptake completely only in purified synaptosomes prepared from cortex. In whole brain synaptosomal fractions, noradrenaline was partially accumulated into dopaminergic neurones; this uptake process was not inhibited by tricyclic antidepressants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00512385
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Nerve terminal ATPase as possible trigger for neurotransmitter release (1975)
    [s.l.] : Nature Publishing Group
    Nature 255 (1975), S. 237-238 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We have already suggested elsewhere7"9 that nerve ending particles (synaptosomes) prepared from rat cerebral cortex contain an ouabain-msensitive component of that ATPase activity which is apparently activated by sodium and potas sium ions in the presence of magnesium ions and which is generally ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/255237a0
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    Paper (German National Licenses)
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