ISSN:
1573-4943
Keywords:
Trichosanthin
;
identification of TCS-NADPH complex
;
difference UV spectroscopy
;
fluorescence spectroscopy
;
31P NMR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Trichosanthin, a type I ribosome-inactivating protein with RNAN-glycosidase activity, forms a stable complex with nicotinamide adenine dinucleotide phosphate, a substrate analog. Difference UV spectroscopy, fluorescence spectroscopy, and31P NMR are used to identify the formation of the complex, followed by a crystal structure analysis carried out to elucidate the active-site structure of trichosanthin. The determination of germinal vesicle breakdown indicates that the complex does not, at least for abortion-inducing activity, result in competitive inhibition to the protein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01980325
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