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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Pediatric anesthesia 13 (2003), S. 0 
    ISSN: 1460-9592
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1572-8889
    Keywords: sperm competition ; sperm longevity ; copulation ; melon fly ; Diptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1572-8889
    Keywords: sperm competition ; sperm longevity ; sterile male release method ; Bactrocera cucurbitae ; Diptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sperm competition inBactrocera cucurbitae was studied by double matings of one female with normal and sterile males, with different intervals between the first and the second matings and with or without allowing oviposition after the first or the second mating. When the interval was less than 4 days, the last-male sperm precedence,P 2 , was not different from 0.5, but as the interval was prolonged,P 2 was higher than 0.5. There was no significant difference between treatments in which females were allowed to oviposit after the first mating and only after the second mating. The reason for the higherP 2 when the interval was long was therefore attributed not to sperm usage for egg fertilization during the two matings but, possibly, to sperm mortality. ThatP 2 was 0.5 for shorter intervals suggests that particular sperm replacement mechanisms such as removal and inactivation are absent in B. cucurbitae. Our study is the first to demonstrate a significant effect of short sperm longevity on the last-male sperm precedence.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4986
    Keywords: Lacto-N-tetraose ; lacto-N-neotetraose ; β-D-galactosidase ; β-1,3-N-acetylglucosaminyltransferase ; LNT, lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4Glc) ; LNnT, lacto-N-neotetraose (Galβ1-4GlcNAcβ1-3Galβ1-4Glc) ; UDP, uridine 5′-diphosphate ; β-gal-3, β-D-galactosidase from Bacillus circulans ATCC 31382 ; β-1,3-GnT β-1,3-N-acetylglucosaminyltransferase ; HPAEC-PAD ; high performance anion exchange chromatography-pulsed amperometric detection ; ATP, adenosine 5′-triphosphate ; EtOH ethanol ; Galβ-oNP ; o-nitrophenyl β-D-galactopyranoside
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4Glc, LNT) and lacto-N-neotetraose (Galβ1-4GlcNAcβ1-3Galβ1-4Glc, LNnT) were enzymatically synthesized by consecutive additions of GlcNAc and Gal residues to lactose. Lacto-N-triose II (GlcNAcβ1-3Galβ1-4Glc) was prepared first by the transfer of GlcNAc from UDP-GlcNAc to lactose by β-1,3-N-acetylglucosaminyltransferase from bovine serum. The resulting lacto-N-triose II was converted into LNT and LNnT utilizing two kinds of β-D-galactosidase-mediated transglycosylations. Thus, β-D-galactosidase from Bacillus circulans ATCC31382 induced regioselective galactosyl transfer from o-nitrophenyl β-D-galactoside to the OH-3″ position of lacto-N-triose II, and commercially available β-D-galactosidase from B. circulans to the OH-4″ position of lacto-N-triose II. These convenient processes are suitable for large-scale preparations of LNT and LNnT. As another method, LNT was directly synthesized from lactose as an initial substance, utilizing lacto-N-biosidase (Aureobacterium sp. L-101)-mediated transglycosylation with Galβ1-3GlcNAcβ-pNP donor.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Population ecology 41 (1999), S. 291-297 
    ISSN: 1437-5613
    Keywords: Key words Diptera ; Genetic variance ; Heritability ; Larval period ; Selection ; Tephritidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A quantitative genetic analysis of rapid evolution of a life history trait has been conducted on the first 24 generations of mass-rearing in the melon fly Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae). The phenotypic variance of larval development time in each generation was divided into genetic and residual components. Mean and phenotypic coefficients of variation of larval development time decreased gradually as generations proceeded as a result of artificial selection for shorter larval period in the mass-rearing procedure. There was a trend that additive genetic coefficients of variation in larval development time decreased with generations. These changes are entirely attributed to genetic responses to laboratory selection under the mass-rearing environment because the population was maintained at a very large size so as to exclude random genetic drift and inbreeding depression, which would be other factors responsible for the observed genetic changes. The residual coefficients of variation in larval development time did not change with generations. Realized heritability of larval development time was low. The heritabilities for larval development time estimated from parent–offspring regression at generations 60 and 70, when the evolutionary plateau was asymptotically reached, were not significantly larger than 0.
    Type of Medium: Electronic Resource
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