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  • 1
    ISSN: 1432-2307
    Keywords: Diabetes mellitus ; Intestinal mucosa ; Microcirculation ; Electron microscope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using intact tissues and vascular corrosion casts, morphological changes in the small intestine of streptozotocin-induced diabetic rats present 1 month after onset were investigated using scanning and transmission electron microscopy. Although there were increases in the height of the villi in the jejunum and not in the ileum, characteristic changes in surface morphology in the diabetic rats were observed only in the ileum. Use of a vascular corrosion cast technique clearly demonstrated the prominent thick marginal vessels of the intestinal villi of the control rats. In the diabetic rats, the central arterioles in the villi were dilated both in the jejunum and the ileum. Marginal vessels were small in the ileum but prominent in the jejunum. Using transmission electron microscopy, decreases in diameter and the number of endothelial fenestra were also evident in the marginal vessels of the ileal villi in the diabetic rats. Thus in rats made diabetic, fine structural alterations of the marginal vessels are induced in the villi of the distal ileum, in addition to quantitative changes in the jejunal villi, presumably related to hyperphagia. The marginal vessels seem to be involved in the regulation of microcirculation of the intestinal villi in the rat.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2307
    Keywords: Stomach ; Submucosal arterioles ; Mucosal blood flow ; Intra-arterial cushions ; Vascular corrosion cast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To observe the branching site of the rat gastric submucosal arteriole toward the mucosal capillary network, we used vascular corrosion casting, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), under various conditions, including the normal state, water immersion restraint stress for 24 h, ethanol-induced ulcer and administration of low and high doses of noradrenaline. In normal rats, the branching site of the submucosal arteriole toward the mucosa was slightly narrowed, as seen in the cast observations. The SEM and TEM observations revealed that this narrowing was due to the presence of the so-called “intra-arterial cushion”. In the restraint stress rats, this narrowing was increased. TEM and direct SEM observation of the intra-arterial cushion showed much the same findings. In the noradrenaline administered rats, similar changes were observed but not so in the rats with an ethanol-induced ulcer. Arterio-venous-anastomoses (AVA) were not observed in the submucosa, under any of the conditions used. We suggest that the submucosal intra-arterial cushions occurring at the branching sites of the submucosal arterioles may play an important role in regulating blood flow to the gastric mucosa.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 434 (1999), S. 159-165 
    ISSN: 1432-2307
    Keywords: Key words Myocardial cells ; Protein malnutrition ; Ultrastructural changes ; I-band fracture ; Streaming of Z-line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Ultrastructural changes in the ventricular myocardial cells in rats fed a low protein diet were examined by electron microscopy. The most striking changes were observed in the I-band region of the sarcomeres, which occurred very occasionally in myofibrils. In the sarcomere affected the I-band region was often fractured and/or disintegrated on one side, leaving an extended space, while the opposing I-band region disappeared along with dislocation of the intact A-band toward the adjacent Z-line. This dislocation was presumably attributed to the elasticity of titins connecting between the end of thick filaments and the Z-line. Fractured I-band regions were often accompanied by the dilated sarcoplasmic reticulum in the close vicinity of them. In some myofibrils the streaming and/or disruption of the Z-line were occasionally observed where disarrangement of thick and thin myofilaments were usually present. The study suggests that the fracture of the I-band region, consisting of actin and titin filaments, and the streaming of the Z-line of myofibrils are due to a proteolytic action of calpain and/or cathepsin L, which are activated by leaked Ca2+ ion and/or by modification of internal circumstances of the cytoplasm induced by a low protein diet, thus resulting in a low cardiac output.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 183 (1991), S. 537-544 
    ISSN: 1432-0568
    Keywords: Synovial membrane ; Development ; Ultrastructure ; Scanning electron microscopy ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The prenatal and postnatal development of the mouse knee joint was investigated by transmission and scanning electron microscopy. In the prenatal stage, following the appearance of a narrow intercellular cleft between two skeletal elements on the 16th fetal day, clefting extended into the lateral synovial mesenchyme. In some regions, the extension of the cleft was very rapid, but in a certain region (future fat pad region), it was somewhat slower. Macrophage-like cells appeared in the synovial mesenchyme on the 16th fetal day, and then increased in number, and were distributed as if they were clustering around the presumptive clefting zone in the future fat pad region on the 17th–18th fetal day. This suggests that macrophage-like cells may participate in joint development, as they phagocytize and remove some kinds of solid extracellular matrix, and facilitate the cleft extension. In the early postnatal stage, scanning electron microscopic observations showed that there were two different types of cell in the synovial lining. One of them exhibited a surface morphology corresponding to that of macrophages: a spherical cell body and numerous pseudopodia. The other type of cell exhibited various cell shapes with many cytoplasmic processes extending along the synovial surface.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 201 (1979), S. 197-205 
    ISSN: 1432-0878
    Keywords: Avian lung ; Pulmonary receptor ; APUD endocrine cells ; Monoamine histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The epithelium of the intrapulmonary airways of the chicken lung has been studied by fluorescence and electron microscopy. Numerous intensely yellow-fluorescent cells occur in the epithelium of the primary and secondary bronchi. The cell cytoplasm contains characteristic granular vesicles with an electron-dense central core. The vesicles react positively to chromaffm and argentaffin treatment, indicating that they are possible storage sites for amines. Synapse-like junctions occur between the granular cells and the intraepithelial nerve endings, filled with numerous mitochondria, suggesting that these granular cells may have a dual function as both receptor and endocrine cell.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 231 (1983), S. 117-128 
    ISSN: 1432-0878
    Keywords: Thoracic aorta ; Basilar artery ; Endothelial junction ; Freeze-fracture ; Permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The thoracic aorta and basilar artery, in which the incidence of atherosclerosis is known to be different, were examined to elucidate the correlation between the structure of the intercellular cleft junction between adjacent endothelial cells and its permeability to HRP. Tannic acid or HRP in the vessel lumen passed through the intercellular clefts of the thoracic aorta into the subendothelial space, whereas in the basilar artery they were unable to penetrate beyond the tight junction of the intercellular clefts. Freeze-fracture replicas revealed that the tight junctions of the thoracic aorta consisted of one to two junctional strands in most areas of the cleaved planes, with discontinuities in some places, whereas those of the basilar artery consisted of a continuous belt-like meshwork of six anastomosing junctional strands on average. These observations confirm that the structure of endothelial junctions in arteries has a close correlation with the permeability of the intercellular clefts to HRP.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0878
    Keywords: Plasma membrane ; Absorptive cells ; Intestine ; Osmoregulation ; Lamprey (Lampetra japonica)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Specific membrane differentiation occurs in the cytoplasmic-tubule system of the absorptive cells lining the mucosa of the lamprey anterior intestine. The absorptive cells are characterized by the presence of abundant mitochondria and a system of well-developed cytoplasmic tubules (∼120 nm in diameter). The cytoplasmic tubules open on to the basolateral cell surface and contain numerous lipoprotein particles (50–100 nm diam.) in their lumina. Lipoprotein particles are also observed in the endoplasmic reticulum and the Golgi complex, and they are transfered to the lateral intercellular space and lamina propria by way of the cytoplasmic tubules. Spirally-wound parallel rows of particles are found in the luminal surface of the cytoplasmic tubules. The rows are ∼17 nm apart and are wound spirally at a pitch of ∼210 nm. Freeze-fracture images of the tubule membranes also show spiral arrays of particles (∼9 nm in diameter) on the P-face, and complementary shallow grooves on the E-face. From these observations, it is suggested that the cytoplasmic-tubule system of the intestinal absorptive cells serves as a channel for the transport of synthesized lipoprotein into the interstitium, and is also the site of the ion and water exchange essential for the maintenance of ionic homeostasis.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 251 (1988), S. 243-248 
    ISSN: 1432-0878
    Keywords: Thoracic duct ; Smooth muscle cell ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The three-dimensional cytoarchitecture and ultrastructure of the smooth muscle cells in the wall of the rat thoracic duct were investigated by scanning and transmission electron microscopy. The muscle layer basically consists of a single layer of circularly arranged cells. The smooth muscle cell is fusiform or ribbon-like in shape, as in veins or venules with a similar or smaller diameter. Connections by spinous processes are observed between adjacent muscle cells along their length. Spot-like membrane contacts frequently occur in areas where facing membranes are closely apposed. These are thought to be gap junctions and may be responsible for electrical coupling and mechanical attachment. Large invaginations arranged regularly in rows on the surface of the smooth muscle cells can be observed. These invaginations are closely associated with a flattened sarcoplasmic reticulum, and caveolae tend to open into the invaginations.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1860-1499
    Keywords: Electron microscopy ; Medium-chain triglyceride ; Intestinal absorption ; Lacteal ; Very low density lipoprotein (VLDL)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The intestinal absorption of a medium-chain triglyceride (MCT) was studied by electron microscopy and biochemical analysis. In jejunal absorptive cells of rats fed tricaprylin, the smooth endoplasmic reticulum in the apical cytoplasm appeared to increase in number and contained one or two particles about 40–80 nm in diameter that were less electron dense and similar in size and profile to very low density lipoprotein. Similar particles were also observed packed in the dilated Golgi sacs and in the extended intercellular spaces. These particles were remarkably increased in number as compared with those in fasted rats. Biochemical analysis of lymph from the main intestinal lymph duct showed that caprylate was apparently demonstrated only in the lymph of rats given tricaprylin at the maximum rate 3h after oral administration. The study strongly suggests that medium-chain triglyceride is at least in part transported via lacteal, possibly in the form of very low density lipoprotein.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The columnar epithelial cells of the intestine in goldfish and rainbow trout were studied by electron microscopy. The most striking feature of the columnar cells, which was common in both fishes, was the extensive formation of lamellar structures in the cytoplasm. These were actually ribbon-like sheets which were bounded by two regular parallel membranes, and were found mainly in the basal half of the cytoplasm. In profile, these lamellar sheets were similar to the basal infoldings in the distal convoluted tubules of kidney, but were independent of the basal plasma membrane. The function of these lamellae is not known; however, these are presumably the structure involved in transport of water or nutrients. The remarkable difference between the goldfish and trout intestine was the occurrence of the invaginations of luminal surface between microvilli, and a variety of vesicles and vacuoles in the apical cytoplasm, observed exclusively in the posterior intestine of goldfish. In the present paper, it is suggested that there are differences in kind or degree of absorption between the goldfish and the rainbow trout, probably between stomachless fish and stomach-possessing fish in general, and that in the former food materials are ingested into the cell of posterior intestine by vigorous pinocytosis.
    Type of Medium: Electronic Resource
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