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  • 1
    Electronic Resource
    Electronic Resource
    IRE1 encodes a putative protein kinase containing a membrane-spanning domain and is required for inositol phototrophy in Saccharomyces cerevisiae (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A novel gene, IRE1, of Saccharomyces cerevisiae was cloned through genetic complementation of a myo- inositol auxotrophic mutant. The predicted amino acid sequence indicated that IRE1 encodes a protein of 126983 Da with two highly hydrophobic regions, probably a signal sequence and a membrane-spanning region. The carboxy-terminal region of IRE1 showed close sequence similarity to the catalytic domains of protein kinases. Disruption of the IRE1 locus caused myo-inositol auxotrophy. The IRE1 product is very likely a protein kinase required for myo-inositol synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb00864.x
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  • 2
    Electronic Resource
    Electronic Resource
    Levels of phosphate buffered saline- and citrate buffer-elutable immunoglobulins in healthy and inflamed gingiva of dogs (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 22 (1987), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The amounts of phosphate buffered saline (PBS)-elutable IgG, IgA, and IgM and citrate buffere-elutable IgG derived from insoluble immune complexes in healthy and inflamed gingiva of dogs were determined. To obtain healthy gingiva, the dogs' teeth were brushed for 6 wk. After achieving clinically healthy gingiva, plaque formation was induced by placement of silk floss ligatures in the cervical area of teeth and inflamed gingiva was obtained after 1 wk and 1 and 3 months of plaque accumulation. The PBS-elutable IgG was measured by single radial immunodiffusion, IgA and IgM by electroimmunoassay, and citrate buffer-elutable IgG by enzyme-linked immunosorbent assay. The PBS-elutable IgG and IgM in inflamed gingiva showed a tendency to increase with the progress of the disease. Particularly, the amount of IgG after 3 months of plaque formation increased to 183.2 μg/100 μg DNA and was significantly higher than that of the healthy gingiva, 88.2 μg/100 μg DNA (p 〈 0.05). The IgM value was highest 1 wk after plaque accumulation. On the other hand, the amount of citrate buffere-lutable IgG in inflamed gingiva was significantly higher than that in healthy gingiva at all experimental periods (p 〈 0.05) and the increase showed a close parallel with the progression of inflammation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1987.tb01598.x
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  • 3
    Electronic Resource
    Electronic Resource
    Interconversion of polyamines in wild-type strains and mutants of yeasts and the effects of polyamines on their growth (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 61 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Yeasts of wild-type strains, such as Saccharomyces cerevisiae, Schizosaccharomyces pombe and Candida albicans were shown to have the ability to form aminopropylcadaverine and aminopropylhomospermidine from cadaverine and homospermidine, respectively. A polyamine autotroph S. cerevisia 179-5, which lacks ornithine decarboxylase, produced both aminopropylcadaverine and aminopropylhomospermidine, while another mutant S. cerevisiae Y 260 A, which lacks spermine synthase, formed only aminopropylcadaverine. Naturally-occurring triamines and tetraamines except norspermidine and norspermine stimulated the growth of S. cerevisiae 179-5. All the six aliphatic diamines with carbon chain length ranging from one to six were effective in activating the growth of S. cerevisiae 179-5, though all of them were not converted to either triamines or tetraamines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03584.x
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  • 4
    Electronic Resource
    Electronic Resource
    Formation of aminopropylhomospermidine from homospermidine in yeasts and thermophilic bacilli (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 48 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract When the yeasts Saccharomyces cerevisiae, Candida albicans and Schizosaccharomyces pombe and the thermophilic bacteria Bacillus stearothermophilus and Bacillus acidocaldarius were cultured in the presence of homospermidine, a new compound accumulated in the cells within a few days. This compound was identified as aminopropylhomospermidine [NH2(CH2)3NH (CH2)4NH(CH2)4NH2] by gas chromatographymass spectrometry (GC-MS) and by the enzymatic cleavage method developed in our laboratories. This polyamine was not produced from homospermidine in Escherichia coli, Bacillus subtilis, Bacillus alkalophilus, or a eukaryotic protozoon, Tetrahymena pyriformis, none of which usually contains appreciable amounts of spermine. These findings suggest that the synthesis of aminopropylhomospermidine from homospermidine is mediated by spermine synthase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02504.x
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  • 5
    Electronic Resource
    Electronic Resource
    Increased phospholipase D activity in human breast cancer (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 280-285 
    Details
    ISSN: 1432-1335
    Keywords: Key words Phospholipase D  ;  Breast tumor  ;  Phosphatidylcholine  ;  Phosphatidylethanol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phospholipase D is believed to play an important role in cell proliferation and tumorigenesis. One of its major functions is to cause a sustained activation of protein kinase C through the primary production of phosphatidic acid from phosphatidylcholine by the enzyme, followed by dephosphorylation forming diacylglycerol. Protein kinase C is known to be activated or translocated in some tumors including breast tumors. In order to examine phospholipase D activity in breast tumors, surgical specimens of human breast tumors were obtained by mastectomy or wide excision, and their phospholipase D activities were assayed by determining the formation of phosphatidylethanol from phosphatidylcholine and ethanol. Phospholipase D activity was predominantly localized in the microsomal fraction of the tumor tissue and markedly stimulated by oleic acid. We observed a significant increase in phospholipase D activity in 17 out of 19 spontaneous human breast tumors as compared to adjacent histologically normal breast tissue. The mean specific activity in the tumors was 52.9 ± 41.8 (SD) pmol min−1 mg protein−1 whereas the value for the normal breast tissue was 34.0 ± 36.2 (SD) pmol min−1 mg protein−1 (P〈0.01; paired Wilcoxon's rank-sum test). The mean tumor/normal activity ratio was 2.37. Among prognostic factors, the nuclear grade, evaluated according to Schnitt et al., was found to be correlated with the activity ratio. Our results suggest a role for phospholipase D in human breast tumors. An elevation in phospholipase D activity is useful as a potential marker for malignant disease in the breast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01208639
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  • 6
    Electronic Resource
    Electronic Resource
    Increased phospholipase D activity in human breast cancer (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 280-285 
    Details
    ISSN: 1432-1335
    Keywords: Phospholipase D ; Breast tumor ; Phosphatidylcholine ; Phosphatidylethanol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phospholipase D is believed to play an important role in cell proliferation and tumorigenesis. One of its major functions is to cause a sustained activation of protein kinase C through the primary production of phosphatidic acid from phosphatidylcholine by the enzyme, followed by dephosphorylation forming diacylglycerol. Protein kinase C is known to be activated or translocated in some tumors including breast tumors. In order to examine phospholipase D activity in breast tumors, surgical specimens of human breast tumors were obtained by mastectomy or wide excision, and their phospholipase D activities were assayed by determining the formation of phosphatidylethanol from phosphatidylcholine and ethanol. Phospholipase D activity was predominantly localized in the microsomal fraction of the tumor tissue and markedly stimulated by oleic acid. We observed a significant increase in phospholipase D activity in 17 out of 19 spontaneous human breast tumors as compared to adjacent histologically normal breast tissue. The mean specific activity in the tumors was 52.9±41.8 (SD) pmol min−1 mg protein−1 whereas the value for the normal breast tissue was 34.0±36.2 (SD) pmol min−1 mg protein−1 (P〈0.01; paired Wilcoxon's rank-sum test). The mean tumor/normal activity ratio was 2.37. Among prognostic factors, the nuclear grade, evaluated according to Schnitt et al., was found to be correlated with the activity ratio. Our results suggest a role for phospholipase D in human breast tumors. An elevation in phospholipase D activity is useful as a potential marker for malignant disease in the breast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01208639
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  • 7
    Electronic Resource
    Electronic Resource
    Construction of a high-throughput rat genetic mapping system with 466 arbitrarily primed-representational difference analysis markers (2000)
    Springer
    Mammalian genome 11 (2000), S. 982-988 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Linkage mapping of quantitative trait loci (QTLs) requires genetic markers that can be efficiently genotyped for a large number of individuals. To isolate genetic markers suitable for this purpose, we previously established the arbitrarily primed RDA (AP-RDA) method. Dot-blotting AP-PCR products (AP-amplicons) onto filters at a high density and hybridization of the filters with the AP-RDA markers made it possible to genotype a large number of individuals simultaneously for multiple loci. In this study, by using 25 primers or primer combinations, we isolated a total of 419 AP-RDA markers by subtracting the AP-amplicon of BUF rats from that of ACI rats, and vice versa. By combining 47 previously isolated markers, a rat genetic map was drawn with 466 AP-RDA markers. Between two given strains of rats other than ACI and BUF, the average informativeness of the markers was 38%. As for the intercross of ACI and BUF rats, 12 selected primers served to genotype 259 loci. In addition, the amounts and quality of genomic DNA to be used for AP-PCR were examined to guarantee reliable genotyping. Now, initial genome scanning of the rat for linkage analysis can be performed efficiently using this mapping system with AP-RDA markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003350010187
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