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  • 1
    Electronic Resource
    Electronic Resource
    DNA-methyltransferase activities in Streptomyces antibioticus (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 55 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Several DNA methyltransferases have been detected in Streptomyces antibioticus ETHZ 7451. One of these enzymes was purified and partially characterized from crude extracts of Streptomyces antibioticus ETHZ 7451. This enzyme only methylates the adenine residues of DNA. However, λ and pBR322 DNAS were not protected ‘in vitro’ with the methylase, indicating that the enzyme does not seem to form part of a restriction-modification system. Remarkably, the efficiency of the enzyme is significantly higher on the DNA isolated from aerial mycelium than from substrate mycelium in S. antibioticus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02798.x
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  • 2
    Electronic Resource
    Electronic Resource
    Flow injection atomic absorption spectrometric determination of iodide using an on-line preconcentration technique (2000)
    Springer
    Fresenius' journal of analytical chemistry 367 (2000), S. 24-28 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A continuous flow atomic absorption spectrometric system was used to develop an efficient on-line preconcentration-elution procedure for the determination of iodide traces. Chromium (VI) is introduced into the flow system and is reduced to chromium (III) in acid medium proportionally to the iodide present in the sample. The Cr(III) reduced by iodide is retained on a minicolumn packed with a poly(aminophosphonic acid) chelating resin, while unreduced Cr(VI) is not retained. Reduced Cr(III) is preconcentrated by passing the sample containing iodide through the system during 3 min, and is then eluted with 0.5 mol L–1 hydrochloric acid and determined by flame atomic absorption spectrometry (FAAS). The detection limit (3σ) obtained is 2.5 μg L–1. Other ions typically present in waters do not interfere. The proposed method allows the determination of iodide in the range 6–220 μg L–1 with a relative standard deviation of 2.7% at a rate of 17 samples h–1. The method has been applied to the determination of iodide in tap and sea waters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160051593
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  • 3
    Electronic Resource
    Electronic Resource
    Indirect atomic absorption spectrometric determination of citric acid by continuous precipitation (1999)
    Springer
    Fresenius' journal of analytical chemistry 365 (1999), S. 370-373 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A continuous precipitation and filtration flow system for the separation of citric acid by precipitation with lead and indirect flame atomic absorption spectrometry is proposed. The precipitate is formed by injecting the lead solution into a carrier containing the sample and is subsequently retained on a filter. By using this reversed precipitation flow-injection configuration, citric acid was determined in the range 2–40 μg mL–1, with a relative standard deviation of 2.9% at a sampling frequency of 60 samples h–1. This method has been applied to the determination of citric acid in fruit juices, carbonated soft drinks and sweets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160051503
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  • 4
    Electronic Resource
    Electronic Resource
    Magnetic resonance imaging and angiography of the brain in embolic left atrial myxoma (1989)
    Springer
    Neuroradiology 31 (1989), S. 137-139 
    Details
    ISSN: 1432-1920
    Keywords: Magnetic resonance imaging ; Cerebral angiography ; Atrial myxoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of left atrial myxoma presenting exclusively with neurological symptoms, studied with magnetic resonance imaging (MRI) combined with cerebral angiography and computed tomography (CT) is reported. Typical angiographic findings suggested the diagnosis of myxoma. MRI showed multiple ischemic lesions disseminated throughout the entire brain, some of which had been clinically asymptomatic. Because of its sensitivity in identifying small cerebral infarcts, MRI should prove in the future to be a first-choice technique in the evaluation of the presence of and extent of cerebral involvement in embolic left atrial myxoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00698841
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  • 5
    Electronic Resource
    Electronic Resource
    Amyloidosis presenting as a perforated giant colonic diverticulum (1999)
    Springer
    European radiology 9 (1999), S. 715-718 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Amyloidosis ; Colon ; Diverticular disease ; Helical CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Colonic amyloidosis is frequent, although rarely radiographically evident. The radiological findings are varied and unspecific. We report a case of perforated giant colonic diverticulum (GCD) diagnosed by helical CT in which amyloid deposition was pathologically demonstrated. The patient was a renal transplant recipient who previously had undergone hemodialysis for 10 years. A possible role of amyloidosis in the pathogenesis of this case is discussed. Giant colonic diverticulum must be included in the spectrum of gastrointestinal manifestations of amyloidosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050739
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  • 6
    Electronic Resource
    Electronic Resource
    Crohn's disease and leukemia (1985)
    Springer
    Digestive diseases and sciences 30 (1985), S. 410-411 
    Details
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01403851
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  • 7
    Electronic Resource
    Electronic Resource
    Lack of correlation between binding of Eco RII methyltransferase to DNA duplexes containing mismatches and the promotion of C to T mutations (1997)
    Springer
    Molecular genetics and genomics 255 (1997), S. 54-59 
    Details
    ISSN: 1617-4623
    Keywords: Key words Mutational hotspots  ;  Cytosine methylation  ;  Mismatch correction  ;  Uracil-DNA glycosylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cytosine methyltransferases (MTases) M. HhaIand M. HpaII bind substrates in which the target cytosine is replaced by uracil or thymine, i.e. DNA containing a U:G or a T:G mismatch. We have extended this observation to the EcoRII MTase (M. EcoRII) and determined the apparent Kd for binding. Using a genetic assay we have also tested the possibility that MTase binding to U:G mismatches may interfere with repair of the mismatches and promote C:G to T:A mutations. We have compared two mutants of M. EcoRII that are defective for catalysis by the wild-type enzyme for their ability to bind DNA containing U:G or T:G mismatches and for their ability to promote C to T mutations. We find that although all three proteins are able to bind DNAs with mismatches, only the wild-type enzyme promotes C:G to T:A mutations in vivo. Therefore, the ability of M. EcoRII to bind U:G mismatched duplexes is not sufficient for their mutagenic action in cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050474
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  • 8
    Electronic Resource
    Electronic Resource
    Three Cases of Tuberculosis after Heart Transplantation in Spain (1998)
    Springer
    European journal of clinical microbiology & infectious diseases 17 (1998), S. 801-806 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Three cases are presented of tuberculosis occurring in a series of 410 heart transplant recipients in a Spanish hospital, representing a rate of 0.73%. Twenty-eight cases reported in the literature are also reviewed. In most series reported, tuberculosis occurred in a small percentage of heart transplant recipients, the average rate being 1.25%. Compared to the general population, a higher percentage (28%) of extrapulmonary and disseminated forms of the disease is seen in these patients. Although a cure without recurrence can usually be achieved with a conventional antituberculous antibiotic regimen, the disaese is still associated with a significant mortality rate of 11%. Guidelines for the early diagnosis and treatment of these patients are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100960050191
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