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  • 1
    Electronic Resource
    Electronic Resource
    Quantitative perturbational molecular orbital analysis of the conformational preference of the aniline molecule and its intramolecular force (II)
    Amsterdam : Elsevier
    Computers and Chemistry 18 (1994), S. 363-369 
    Details
    ISSN: 0097-8485
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0097-8485(94)80028-6
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  • 2
    Electronic Resource
    Electronic Resource
    Tagging genes for blast resistance in rice via linkage to RFLP markers (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 471-476 
    Details
    ISSN: 1432-2242
    Keywords: Disease resistance ; Molecular markers ; Oryza sativa L. ; Pyricularia oryzae Cav. ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Both Pi-2(t) and Pi-4(t) genes of rice confer complete resistance to the blast fungal pathogen Pyricularia oryzae Cav. As economically important plant genes, they have been recently characterized phenotypically, yet nothing is known about their classical linkage associations and gene products. We report here the isolation of DNA markers closely linked to these blast resistance genes in rice. The DNA markers were identified by testing 142 mapped rice genomic clones as hybridization probes against Southern blots, consisting of DNA from pairs of nearly isogenic lines (NILs) with or without the target genes. Chromosomal segments introgressed from donor genomes were distinguished by restriction fragment length polymorphisms (RFLPs) between the NILs. Linkage associations of the clones with Pi-2(t) and Pi4(t) were verified using F3 segregating populations of known blast reaction. Cosegregation of the resistant genotype and donor-derived allele indicated the presence of linkage between the DNA marker and a blast resistance gene. RFLP analysis showed that Pi-2(t) is closely linked to a single-copy DNA clone RG64 on chromosome 6, with a distance of 2.8+1.4(SE) cMorgans. Another blast resistance gene, Pi-4(t), is 15.3+4.2(SE) cMorgans away from a DNA clone RG869 on chromosome 12. These chromosomal regions can now be examined with additional markers to define the precise locations of Pi-2(t) and Pi-4(t). Tightly linked DNA markers may facilitate early selection for blast resistance genes in breeding programs. These markers may also be useful to map new genes for resistance to blast isolates. They may ultimately lead to the cloning of those genes via chromosome walking. The gene tagging approach demonstrated in this paper may apply to other genes of interest for both monogenic and polygenic traits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219436
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  • 3
    Electronic Resource
    Electronic Resource
    Pollen fertility restoration by nuclear gene Fr in CMS common bean: an Fr linkage map and the mode of Fr action (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1056-1062 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris L ; Fr-directed mitochondrial alteration ; Fr linkage map ; Semidominant effect ; Map-based cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Fr gene in common bean, Phaseolus vulgaris L., is a unique gene for the study of plant nuclear-mitochondrial interactions because it appears to directly influence plant mitochondrial genome structure, resulting in the restoration of pollen fertility in cytoplasmic male sterile plants. This gene action is distinct from other pollen fertility restoration systems characterized to date. As a first step towards the map-based cloning of this unusual nuclear gene, we identified RAPD markers linked to Fr using bulked segregant analysis of near-isogenic lines. Using DNA gel blot hybridization, we localized the identified RAPD markers to a linkage group on the common bean RFLP map and constructed a linkage map of the Fr region using both RAPD markers and RFLP markers. Analysis of the mode of Fr action with the aid of identified Fr-linked DNA markers indicated that Fr functions in a semidominant fashion, showing dosage effect in controlling the dynamics of a heteroplasmic mitochondrial population. We also present our observations on the developmental distinctions, crucial in the accurate mapping of the Fr gene, between spontaneous cytoplasmic reversion and Fr-driven fertility restoration, two phenomena that are phenotypically indistinguishable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222921
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular mapping of genes for resistance to rice blast (Pyricularia grisea Sacc.) (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 859-863 
    Details
    ISSN: 1432-2242
    Keywords: Disease resistance ; Rice (Oryza sativa L.) ; Blast (Pyricularia grisea Sacc.) ; Restriction fragment length polymorphism (RFLP) ; Gene mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two dominant genes conferring complete resistance to specific isolates of the rice blast fungus, Pyricularia grisea Sacc., were located on the molecular map of rice in this study. Pi-l(t) is a blast resistance gene derived from the cultivar ‘LAC23’. Its map location was determined using a pair of nearly isogenic lines (NILs) and a B6F3 segregating population from which the isoline was derived. RFLP analysis showed that Pi-l(t) is located near the end of chromosome 11, linked to RZ536 at a distance of 14.0±4.5 centiMorgans (cM). A second gene, derived from the cultivar ‘Apura’, was mapped using a rice doubled-haploid (DH) population. This gene was located on chromosome 12, flanked by RG457 and RG869, at a distance of 13.5+-4.3 cM and 17.7+-4.5 cM, respectively. The newly mapped gene on chromosome 12 may be allelic or closely linked toPi-ta. (=Pi-4(t)), a gene derived from Tetep that was previously reported to be linked to RG869 at a distance of 15.4±4.7 cM. The usefulness of markers linked to blast resistance genes will be discussed in the context of breeding for durable blast resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224086
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  • 5
    Electronic Resource
    Electronic Resource
    Recovery of transgenic rice plants expressing the rice dwarf virus outer coat protein gene (S8) (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 522-527 
    Details
    ISSN: 1432-2242
    Keywords: Key words RDV ; Segment S8 ; Rice ; Transgenic plants ; Particle bombardment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The coding region of the eighth largest segment (S8) of the rice dwarf virus (RDV) was obtained from a RDV Fujian isolate. It was then cloned into pTrcHisA for expression in E. coli and into vector pE3 for plant transformation. By using callus derived from mature rice embryos as the target tissue, we obtained regenerated rice plants after bombardment of the former with plasmid pE3R8 containing the RDV S8 gene and the marker gene neomycin phosphotransferase (NPT II). Southern blotting confirmed the integration of the RDV S8 gene into the rice genome. The expression of the outer coat protein in both E. coli and rice plants was confirmed by western blotting. The recovery of transgenic rice plants expressing S8 gene is an important step towards studying the function of the RDV genes and obtaining RDV-resistant rice plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050446
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular mapping of rice chromosomes (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 815-829 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa ; Molecular markers ; RFLP ; Genetic map ; Trisomies ; DNA methylalion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report the construction of an RFLP genetic map of rice (Oryza sativa) chromosomes. The map is comprised of 135 loci corresponding to clones selected from a PstI genomic library. This molecular map covers 1,389 cM of the rice genome and exceeds the current classical maps by more than 20%. The map was generated from F2 segregation data (50 individuals) from a cross between an indica and javanica rice cultivar. Primary trisomics were used to assign linkage groups to each of the 12 rice chromosomes. Seventy-eight percent of the clones assayed revealed RFLPs between the two parental cultivars, indicating that rice contains a significant amount of RFLP variation. Strong correlations between size of hybridizing restriction fragments and level of polymorphism indicate that a significant proportion of the RFLPs in rice are generated by insertions/delections. This conclusion is supported by the occurrence of null alleles for some clones (presumably created by insertion or deletion events). One clone, RG229, hybridized to sequences in both the indica and javanica genomes, which have apparently transposed since the divergence of the two cultivars from their last common ancestor, providing evidence for sequence movement in rice. As a by product of this mapping project, we have discovered that rice DNA is less C-methylated than tomato or maize DNA. Our results also suggest the notion that a large fraction of the rice genome (approximately 50%) is single copy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273666
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  • 7
    Electronic Resource
    Electronic Resource
    Probing R-parity violation in the production of $t \bar c$ $(c \bar{t})$ at lepton colliders (2000)
    Springer
    The European physical journal 16 (2000), S. 695-700 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. We studied the process $e^{+}e^{-} \rightarrow t\bar c+c\bar{t}$ in a $R_{p}$ -violating supersymmetric model with effects from both B- andL-violating interactions. The calculation shows that it is possible to either detect the $R_{p}$ -violating signal at the Next Linear Collider or get more stringent constraints on the heavy-flavor $\rlap/R_{p}$ couplings. A comparison with results from $\gamma\gamma \rightarrow t\bar c+ c\bar{t}$ may allow one to distinguish between B- and L-violating interactions. For very clean background conditions and $R_{p}$ -violating parameters close to the present limits, the future detection of B-violating interactions should be possible. The process of $\mu ^{+} \mu ^{-} \rightarrow t \bar c+c\bar{t}$ is also considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100520000381
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