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  • 1
    Electronic Resource
    Electronic Resource
    Analysis by high-performance liquid chromatography of free amino acids extracted from needles of drought-stressed and shaded Pinus ponderosa seedlings (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 79 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Free amino acid concentrations in needles of drought-stressed and shaded seedlings of ponderosa pine (Pinus ponderosa Dougl. ex Laws.) were analyzed to determine the influence of low irradiance on a biochemical response to drought stress. A convenient method was developed for separating and quantitating amino acids in needle extracts using derivatization with dansyl chloride and reversed-phase high-performance liquid chromatography (HPLC). Twenty-one amino acids were separated on an Ultrasphere ODS CIS column and detected with a fluorescence spectrophotometer. As little as 10 pmol of each were detected with good peak separation and reproducible retention times. The results of HPLC analysis showed that drought and shading induced an increase in total amino acid concentrations in needles; shading had the greater effect. Arginine and proline concentrations increased most in needles of drought-stressed seedlings and remained high in unshaded seedlings recovering from drought 48 h after rewatering. Arginine and glutamine increased most in the shaded seedlings, which did not survive severe drought. The large increase in arginine in both drought-stressed and severely shaded seedlings suggests that sequestering and storage of ammonia are important when stress reduces carbon fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb05861.x
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of flurprimidol on bud flush, shoot growth, and on endogenous gibberellins and abscisic acid of Douglas-fir seedlings (1994)
    Springer
    Journal of plant growth regulation 13 (1994), S. 131-136 
    Details
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract For 4-month-old Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] seedlings under 17-h photoperiods in controlled environment conditions, flurprimidol (α-(1-methlyethyl-α-[4-(trifluoromethoxy)phenyl]-5-pyrimidine-methanol) decreased incidence of second flushing from 81 to 54% and significantly reduced height growth in the second year following low-temperature treatment, in comparison to untreated controls. For seedlings under 15-h photoperiods, flurprimidol had only limited early effects, but after a period of bud dormancy, shoot growth was reduced to one-third that of the untreated controls. Under both photoperiods flurprimidol significantly depressed abscisic acid levels and gibberellin-like bioactivity. These results suggest that growth reduction in this conifer by flurprimidol may well involve inhibition of gibberellin biosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196376
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence procedures (1980)
    Springer
    Planta 150 (1980), S. 366-370 
    Details
    ISSN: 1432-2048
    Keywords: Auxin (analysis) ; High performance liquid chromatography ; Pseudotsuga ; Spectrofluorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The use of spectrofluorimeter coupled to a reverse phase high performance liquid chromatography column permits selective detection of indole-3-acetic acid at the low picogram level. The value of the technique is demonstrated by the analysis of endogenous IAA in elongating shoots, xylem sap and callus of Douglas-fir. The data are also used to illustrate a procedure whereby the accuracy of chromatographic analyses can be verified within definable probability limits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390171
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  • 4
    Electronic Resource
    Electronic Resource
    A liquid cytokinin pulse induces adventitious shoot formation from Douglas-fir cotyledons (1991)
    Springer
    Plant cell reports 10 (1991), S. 156-160 
    Details
    ISSN: 1432-203X
    Keywords: Douglas-fir ; Tissue culture ; Adventitious buds ; Cytokinin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of high-concentration, 2-h liquid pulses of N6-benzylaminopurine (BA) and thidiazuron (TD) on adventitious bud and shoot formation were tested in cotyledons of Douglas-fir (Pseudotsuga menziesii). Seedling age proved important; on average, cotyledons from the youngest seedlings formed 10-fold more buds than cotyledons from the oldest seedlings. Optimal cytokinin concentrations for the youngest cotyledons were 400 and 800 μM BA, and 100 and 200 μM TD. Shoots developed best from buds induced with 300, 400, and 800 μM BA. Four gelling agents were tested; BRL agarose yielded more than three times the number of buds, and Gelrite nearly twice the number of buds, as either Sigma agar or Difco Bacto-Agar. One of the best treatments (400 μM BA, agarose) yielded more cotyledons with buds, and more buds per cotyledon, than when cytokinins were incorporated into the growth medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232049
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  • 5
    Electronic Resource
    Electronic Resource
    Transient gene expression of microprojectile-introduced DNA in Douglas-fir cotyledons (1991)
    Springer
    Plant cell reports 10 (1991), S. 517-521 
    Details
    ISSN: 1432-203X
    Keywords: Biolistics-GUS-355-Conifer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasmid DNA containing the reporter gene uidA encoding β-glucuronidase (GUS), driven by the cauliflower mosaic virus 35S promoter, was introduced on high-velocity microprojectiles into cultured cotyledons of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. Transient gene expression was measured by counting the number of distinct loci of GUS activity per cotyledon. Contrary to published results on angiosperms, repeated bombardments did not increase expression in Douglas-fir. Expression varied significantly among cotyledons from different seedlings. The amount of time between DNA delivery and treatment of cotyledons with auxins and cytokinins strongly affected GUS expression. The optimal cytokinin pretreatment produced an average of 20 loci per cotyledon. In several experiments, more than 95% of the treated cotyledons exhibited at least some transient expression. Expression remained constant up to three days following DNA delivery into cotyledons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234585
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    Paper (German National Licenses)
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