ISSN:
0275-3723
Keywords:
polymorphonuclear leukocyte
;
rabbit
;
tannic acid-glutaraldehyde
;
ionophore
;
A23187
;
degranulation
;
membrane fine structure
;
Chemistry
;
Molecular Cell Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
TAG fixation of normal and Ca2+ ionophore-treated rabbit polymorphonuclear leukocytes (PMN) has revealed membrane components not apparent with conventional glutaraldehyde fixation. These included a 30 Å external electron-dense coating on untreated cells. A somewhat thicker coat (40 Å) was observed in ionophore-treated, nondegranulating PMN. In ionophore-treated, degranulating PMN, a 65 Å cell membrane coat was observed. A similar coat was observed on the inner side of the membrane of some azurophil-type granules, but the electron density and thickness were not so pronounced. Cytoplasmic granules were often closely apposed and often protruded outward at the plasma membrane. Extracellular lamellae, sometimes stacked apposed to the plasma membrane, possibly represent remnants of intense granule extrusion. Sequential degranulation of the respective granules was not apparent.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jsscb.1981.380160206
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