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  • 1
    Electronic Resource
    Electronic Resource
    Purification and partial characterization of a protease associated with photosystem II particles (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 95 (1995), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A protease was extracted with 1 M NaCl from spinach (Spinacia oleracea L.) photosystem II (PSII) particles and purified through gel filtration and anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis of the protease revealed a polypeptide with a molecular mass of 43 kDa. The activity of the purified protease was assayed using a 24 kDa water-soluble protein as substrate, visualized through SDS-PAGE. The protease even remained active in the presence of 0.1 and 0.2 M NaCl, although the degradation pattern changed, which indicated that the protease was different from that reported earlier by another group. The presence of 0.3 M NaCl was shown to be inhibitory. The protease was inhibited by 1,10-phenanthroline and EGTA-NaOH (pH 7.0), indicating that the metal ions are essential for activity and that the enzyme is a metal-protease. FTIR spectroscopy was used to examine the conformationally sensitive amide I' bands of the protease. The protease was observed to undergo spectroscopic changes that reflect the conformational changes that take place when Ca2+ is bound, which further confirms that the protease is a metal-protease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1995.tb05527.x
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  • 2
    Electronic Resource
    Electronic Resource
    A Combined Ultrasonic Wave Nondestructive Testing Technique for Defect Detection in Concrete (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 41-42 (Apr. 2008), p. 297-302 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We describe the design and construction about the Ultrasonic method to determine thedefects in concrete structure. The wave velocity and its energy are different when it transfers in theconcrete material with various defects. The defects can then be detected according to the relativewave velocity and energy variations in concrete material. In the present paper, two methods containboth the wavelet analysis and the nerve network used to determine the relative wave energy changein concrete, and then we will evaluate the defects in material by energy change. The result showsthat the present method can give an accurate measurement for the defects in concrete structure
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/40/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.41-42.297.pdf
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Some liminf results on increments of fractional Brownian motion (1996)
    Springer
    Acta mathematica hungarica 71 (1996), S. 215-240 
    Details
    ISSN: 1588-2632
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00052111
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A possible calcium binding site in D1 protein: A fluorescence and FTIR study of the interaction between lanthanides and a synthetic peptide (1995)
    Springer
    Photosynthesis research 44 (1995), S. 297-302 
    Details
    ISSN: 1573-5079
    Keywords: calcium binding site ; conformation ; D1 fragment ; lanthanides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A peptide ranging from residue 229 to 240 of the D1 protein of Photosystem (PS) II was synthesized and lanthanides were used as candidates of calcium. Fluorescence and FTIR spectroscopy were used to test the conformational adaptation after lanthanide additions. Fluorescence spectroscopy showed that the synthetic peptide provides lanthanide binding site, and that glutamic acids are involved in lanthanide binding. Resolution enhancement techniques were combined with band curve-fitting procedures to quantitate the FTIR spectral information from the amide 1 bands. The relative areas of these component bands indicate that lanthanide induced a substantial decrease in the amount of unordered structure and turns, while a corresponding increase in the amount of α-helix and ‘open loop’ was also observed. This indicates that a relatively compact structure of the synthetic peptide is formed if lanthanides are applied. The results may reflect on the physiological and biochemical function of calcium in PS II, including preventing D1 from trypsin digestion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048603
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Fluorescence and Fourier-transform infrared spectroscopic studies on the role of disulfide bond in the calcium binding in the 33 kDa protein of Photosystem II (1996)
    Springer
    Photosynthesis research 48 (1996), S. 379-384 
    Details
    ISSN: 1573-5079
    Keywords: calcium binding ; disulfide bond ; FTIR ; 33 kDa protein ; lanthanide ; Photosystem II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 33 kDa protein of Photosystem II has one intrachain disulfide bond. Fluorescence spectroscopy shows that the major groups in the protein that bind to Ca2+ should be the carboxylic side groups of glutamic acid and/or aspartic acid. Fluorescence and Fourier-transform infrared (FTIR) spectroscopic studies indicate that the conformation of the 33 kDa protein is altered upon reduction, while the reduced protein still retains the secondary structure. FTIR spectroscopy also shows that the metal ions induce a relative decrease of unordered structure and β-sheet, and a substantial increase of α-helix in both the intact and the reduced 33 kDa protein. This indicates that the addition of cations results in a much more compact structure and that both the intact and the reduced 33 kDa proteins have the ability to bind calcium. The above results may suggest that the disulfide bridge is not essential for calcium binding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029470
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    Paper (German National Licenses)
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