Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    β-Arrestin1 and β-arrestin2 are differentially required for phosphorylation-dependent and -independent internalization of δ-opioid receptors (2005)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 95 (2005), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: β-Arrestins are key negative regulators and scaffolds of G protein-coupled receptor (GPCR) signalling. β-Arrestin1 and β-arrestin2 preferentially bind to the phosphorylated GPCRs in response to agonist stimulation, resulting in receptor internalization and desensitization. The critical roles of GPCR kinases (GRKs)-catalyzed receptor phosphorylation and interaction of β-arrestins with the phosphorylated receptor in receptor internalization are well established. However, emerging evidence suggests that an agonist-stimulated internalization mechanism that is independent of receptor phosphorylation may also be employed in some cases, although the molecular mechanism for the phosphorylation-independent GPCR internalization is not clear. The current study investigated the role of receptor phosphorylation and the involvement of different β-arrestin subtypes in agonist-induced δ-opioid receptor (DOR) internalization in HEK293 cells. Results from flow cytometry, fluorescence microscopy, and surface biotin labelling experiments showed that elimination of agonist-induced DOR phosphorylation by mutation GRK binding or phosphorylation sites only partially blocked agonist-induced receptor internalization, indicating the presence of an agonist-induced, GRK-independent mechanism for DOR internalization. Fluorescence and co-immunoprecipitation studies indicated that both the wild-type DOR and the phosphorylation-deficient mutant receptor could bind and recruit β-arrestin1 and β-arrestin2 to the plasma membrane in an agonist-stimulated manner. Furthermore, internalization of both the wild-type and phosphorylation-deficient receptors was increased by overexpression of either type of β-arrestins and blocked by dominant-negative mutants of β-arrestin-mediated internalization, demonstrating that both phosphorylation-dependent and -independent internalization require β-arrestin. Moreover, double-stranded RNA-mediated interference experiments showed that either β-arrestin1 or β-arrestin2 subtype-specific RNAi only partially inhibited agonist-induced internalization of the wild-type DOR. However, agonist-induced internalization of the phosphorylation-deficient DOR was not affected by β-arrestin1-specific RNAi but was blocked by RNAi against β-arrestin2 subtype. These data indicate that endogenous β-arrestin1 functions exclusively in the phosphorylation-dependent receptor internalization, whereas endogenous β-arrestin2, but not β-arrestin1, is required for the phosphorylation-independent receptor internalization. These results thus provide the first evidence of different requirement for β-arrestin isoforms in the agonist induced phosphorylation-dependent and -independent GPCR internalization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.2005.03352.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Charge dynamics in silicon nitride/silicon oxide double layers (2001)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 78 (2001), S. 2757-2759 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Transport of positive charge deposited by a corona discharge on the open nitride surface of a silicon nitride (150 nm)/silicon oxide (300 nm) double layer on silicon substrate is studied by determining the location of the charge centroid with a capacitance–voltage method used in conjunction with surface-potential measurements. At temperatures of about 400 °C, the charge is mobile in the nitride and a large part of it is eventually trapped at the nitride/oxide interface while some of the charge reaches the substrate through the oxide. Indications are that at this temperature the mean free path of positive charges in the nitride and oxide layers is comparable to the layer thicknesses, but much shorter in the nitride at lower temperatures. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1369387
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Molecular motion in a blend of poly(vinylphenol) and poly(ethylene oxide) as studied by high-resolution solid-state carbon-13 NMR spectroscopy (1993)
    s.l. : American Chemical Society
    Macromolecules 26 (1993), S. 2198-2201 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00061a009
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Phase Structures of Hexamine Cross-Linked Novolac Blends. 1. Blends with Poly(methyl methacrylate) (1994)
    s.l. : American Chemical Society
    Macromolecules 27 (1994), S. 4919-4926 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00096a012
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Composition dependence of the miscibility and phase structure of amorphous/crystalline polymer blends as studied by high-resolution solid-state carbon-13 NMR spectroscopy (1992)
    s.l. : American Chemical Society
    Macromolecules 25 (1992), S. 2336-2340 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00035a009
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Poly(vinylphenol)/poly(methyl acrylate) and poly(vinylphenol)/poly(methyl methacrylate) blends: hydrogen bonding, miscibility, and blending effects on molecular motions as studied by carbon-13 CP/MAS NMR (1991)
    s.l. : American Chemical Society
    Macromolecules 24 (1991), S. 5756-5762 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00021a004
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Nuclear Overhauser effect study on intermolecular interaction and miscibility of polymer blends in the solution state (1992)
    s.l. : American Chemical Society
    Macromolecules 25 (1992), S. 4871-4875 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00045a007
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Determination of molecular weight distributions of novolac resins by gel permeation chromatography (1997)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 35 (1997), S. 1399-1407 
    Details
    ISSN: 0887-624X
    Keywords: phenol-formaldehyde resin; ; novolac ; GPC ; molecular weight averages ; NMR ; hydrodynamic volume ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The number- and weight-average molecular weights of several statistical and high ortho novolac resins were determined using gel permeation chromatography (GPC). The standards used were pure compounds having between 2 and 12 phenol units bridged via methylene linkages. Three series of compounds were studied: (i) those with methylene linkages substituted in only the ortho positions relative to the phenolic hydroxyl group; (ii) those in which all para positions, together with sufficient ortho positions, were used to synthesize the compounds; and (iii) those in which the methylene linkages were substituted at a mixture of ortho and para positions. Such compounds, having known molecular architecture and units similar to the segments of industrial novolac resins, provide for a more exact measurement of the molecular weight than do the commonly used poly(styrene) standards. Using these new standards the number average molecular weights of the resins determined by GPC were in good agreement with the average molecular weight obtained by 1H-NMR spectra of the resins, particularly for low molecular weight resins. GPC analysis of higher molecular weight resins tends to underestimate the molecular weights because of complications introduced by hydrogen bonding. © 1997 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 35: 1399-1407, 1997
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 9
    Electronic Resource
    Electronic Resource
    Chemistry of novolac resins. II. Reaction of model phenols with hexamethylenetetramine (1997)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 35 (1997), S. 1389-1398 
    Details
    ISSN: 0887-624X
    Keywords: benzylamines ; benzoxazines ; curving ; HMTA ; NMR ; novolac resins ; xylenols ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: It is proposed that the reactions of hexamethylenetetramine (HMTA) with 2,4-xylenol and with 2,6-xylenol occur by different pathways. The rate of reaction and the final product distribution depend on the initial xylenol : HMTA ratio and are different in the two systems. Measured by HMTA consumption, with 2,4-xylenol the reaction rate increased with increasing xylenol : HMTA ratios, whereas with 2,6-xylenol the rate of reaction decreased with increasing 2,6-xylenol : HMTA ratio. In systems which contain both 2,4- and 2,6-xylenol, a strong preference for reaction of the HMTA with the ortho site of 2,4-xylenol was noted. This preference was apparent even in mixtures in which 2,6-xylenol was present in greater amounts than 2,4-xylenol. © 1997 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 35: 1389-1398, 1997
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 10
    Electronic Resource
    Electronic Resource
    The reaction of furfuryl alcohol resins with hexamethylenetetramine: A 13C and 15N high-resolution solid-state NMR study (1997)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 35 (1997), S. 2233-2243 
    Details
    ISSN: 0887-6266
    Keywords: furfuryl alcohol resins ; crosslinking reactions ; hexamethylenetetramine ; high-resolution solid-state NMR ; Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The reactions between furfuryl alcohol (FA)/poly(furfuryl alcohol) (PFA) and hexamethylenetetramine (HMTA) have been studied by 13C and 15N high-resolution solid-state NMR techniques. Highly crosslinked polymer networks similar to those obtained from the thermal curing of FA resins under acidic condition are formed. Possible reaction pathways are postulated on the basis of changes of chemical structures during the curing; α-substituted furfurylamines are shown to be the initial intermediates. Their further reactions with FA/PFA, together with thermal decomposition, produce methylene linkages between furan rings, resulting in chain extension and crosslinking, which occurs at both the methylene linkages and the 3- or 4-C of furan rings. Various side-products such as amines, imines, amides, imides, and nitriles are also formed during the reactions, and some of these can remain in the resins up to 205°C. The crosslinked network can be heterogeneous with different structures on nanometer scales when higher oligomers of FA resins react with HMTA. © 1997 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 35: 2233-2243, 1997
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...