ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Aminoacyl-tRNA synthetase and tRNA were isolated from the chrysomonad Ochromonas danica. The mutual effect of methionine and ethionine, and the effect of other amino acids on methionyl- and ethionyl-tRNA formation, were tested in an in vitro system.The tRNAMet had a similar accepting capacity for either methionine or ethionine. Ethionine and methionine, but none of the other amino acids tested, competed for the same aminoacyl-tRNA synthetase. The Km of methionine was 0.88 × 10–5 M, and that of ethionine 5 × 10–4 M. Ethionine inhibited methionine binding; Ki 3.4 × 10–4 M. The respective values in a similar system isolated from E. coli were 2.2 × 10–5, 1.95 × 10–3, and 1.95 × 10–3.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.1973.tb06023.x
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