ISSN:
1432-1912
Keywords:
Key words Gastrin
;
Cholecystokinin
;
CCKB receptors
;
Kidney
;
Glomeruli
;
Collecting duct
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Two types of receptors for gastrin and cholecystokinin (CCK) have been identified in the gastrointestinal tract and in the central nervous system: CCKA and CCKB receptors. Here we report evidence for the expression of CCKB receptors in the guinea-pig kidney. Specific binding sites for [125I]gastrin were detected in sections of the guinea-pig kidney: Binding was saturable, pH-, temperature- and time-dependent, and specific for gastrin-related peptides. The potencies for inhibition of binding of [125I]gastrin were CCK-8 〉 gastrin 17-I 〉 CCKB receptor antagonist L-365,260 〉 des(SO3)CCK-8 〉 CCKA receptor antagonist L-364,718. Autoradiography demonstrated specific [125I]gastrin binding to medullary collecting ducts and to a much lesser extent to glomeruli, but not over other structures. CCKB receptor cDNA fragments were amplified by RT-PCR from total kidney, isolated tubuli and from tissues known to express CCKB receptors such as stomach and brain. The kidney might therefore be a previously unidentified site of action for gastrin and cholecystokinin-related peptides.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00005255
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