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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 65 (1987), S. 469-474 
    ISSN: 1432-1440
    Keywords: Lente carbohydrates ; Blood glucose control ; Type II diabetes ; Bread
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Blood glucose responses to test meals containing 75 g of different breads were compared in 103 type II diabetic patients under sulfonyl urea treatment. Nine breads differing in type of cereal, physical structure, and dietary fiber content were studied using pairwise intraindividual comparisons. It could be shown that the type of cereal and the fiber content had no significant influence upon the maximal or mean postprandial blood glucose increase. Coarse-grained breads, however, resulted in significantly lower blood glucose responses than breads that were finer grained or were produced from flour. Physical properties determining the access of intestinal hydrolytic enzymes to ingested carbohydrates seem to be major factors that influence postprandial blood glucose responses.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Bradyrhizobium japonicum mutant 132 was obtained by random TnphoA mutagenesis of strain 110spc4. A 6.5 kb BamHI kanamycin-resistance-coding DNA fragment of mutant 132 was used as a hybridization probe to clone the corresponding wild-type fragment. DNA sequence analysis of a 3213 bp BamHI—ClaI fragment revealed that three open reading frames (ORFs) were encoded in the same orientation. Based on sequence similarities to other proteins in the database, the second ORF was called sipS (signal peptidase). The TnphoA insertion in mutant 132 was found to be in frame near the 3′ end of sipS. The resulting SipS—PhoA hybrid polypeptide was shown to be expressed in free-living B. japonicum and in Escherichia coli cultures. An immunoblot analysis with a polyclonal antibody directed against the alkaline phosphatase revealed the appearance of a weak signal of a 70 kDa polypeptide both in B. Japonicum and in E. coli, in agreement with the calculated size of the hybrid polypeptide. A much stronger 52 kDa band was also detected. Mutant 132 was specifically disturbed in the interaction with soybean (Glycine max) when the bacteria were released from the infection threads. The bacteroids were not stably maintained within the symbiosome. Numerous vesicles were found in the plant cytosol, which finally resulted in the formation of large vacuoles within the infected nodule cells. Immunohistochemical analyses with antibodies directed against nodulins of the peribacteroid membrane indicated a lower expression of these proteins. The mutant phenotype was genetically complemented by 4.4 kb BamHI fragment including sipS. Subfragments thereof also complemented a temperature-sensitive E. coli lepB mutant, demonstrating that the B. japonicum fragment was functionally replacing Lepts in E. coli. Genetic studies suggested that the three genes are organized in one common operon which is expressed from a promoter upstream of the sequenced region. Inactivation of the gene downstream of sipS did not result in a detectable phenotype.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of neurology 177 (1957), S. 92-102 
    ISSN: 1432-1459
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Die gestellten Fragen werden abschließend folgendermaßen beantwortet: Auch in der Spätphase von Hirnverletzungen sollte wenigstens einmal eine eingehende hirnelektrische Untersuchung erfolgen, wenn auch die klinische Diagnose eindeutig und der Verlauf unkompliziert erscheinen. Auf Grund unserer EEG-Befunde konnten wir in etwa 10% die klinische Diagnose erweitern bzw. korrigieren. Beim Bestehen von Anfällen nach Hirnschädigungen und bei Verläufen, die nicht der Erfahrung bei gleichartigen Verletzungen entsprechen, ist die Anfertigung eines EEGs unerläßlich. Die EEG-Befunde bei gedeckten und offenen Hirnschädigungen wiesen insofern eindeutige Unterschiede auf, als bei gedeckten Traumen Allgemeinveränderungen und bei offenen Herdbefunde überwogen.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 253 (1996), S. 481-487 
    ISSN: 1434-4726
    Keywords: Bone plates ; Bone screws ; Laryngeal cartilage ; Laryngeal reconstructive surgery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of this study was to investigate and develop techniques for stabilization and reconstruction of laryngeal defects with a method similar to osteosynthesis. In an anatomical study, 400 extrusion forces of sutures and various screws (Howmedica) were measured in the thyroid cartilage of fresh cadavers. A new screw was then especially designed for cartilage and a new technique was developed for stabilizing cartilage, using a screw-nut made out of bone. To date, 30 patients have undergone chondrosynthetic reconstruction of the larynx. Measurements of extrusion forces were found to depend on the degree of calcification present in the area of the laryngeal skeleton examined and the type of fixation device used. Good clinical results were achieved in all 30 patients studied and depended on stabilization of the two sides of the thyroid cartilage after thyrotomies, bridging of laryngeal defects and splinting of laryngeal fractures. Besides the increased extrusion forces the advantages of chondrosynthesis lay within the possibilities of axial stabilization and exact bridging of defects with or without implants.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 254 (1997), S. 463-465 
    ISSN: 1434-4726
    Keywords: Laryngeal cartilage ; Laryngeal reconstructive surgery ; Chondrosynthesis ; Plate and screw fixation systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two patients treated for laryngeal tumors at the University of Lübeck are presented. Both underwent laryngeal reconstructions with plate and screw fixation systems immediately following partial laryngectomies. Recurrent disease was found at 14 and 55 months, respectively, and was subsequently managed by total laryngectomy. The laryngectomy specimens with the plate and screws in situ were evaluated histopathologically, paying special attention to the reaction of local tissue to the implants. Findings showed that the metallic plates were fixed with screws in the bony trabeculae of the laryngeal skeleton. The histological response showed primary wound healing without evidence of sequestration or implant loosening, as often associated with excess mechanical stress. Furthermore, there were no signs of tissue necrosis or widespread chronic inflammation. In both patients, a small discrete area of chronic inflammation and soft tissue fibrosis was noted adjacent to the screw heads. This study supports our previously documented clinical experience that the use of chondrosynthetic techniques is almost complication-free and is an ideal system for performing precise anatomical reconstruction of the laryngeal skeleton.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 40 (1980), S. 45-54 
    ISSN: 1434-6036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Splitting and renormalisation of doubly-degenerate optical phonons in Ce c La1−c F3 single crystals (0≦c≦1) has been observed in Raman-scattering experiments. The effects depend on the 4f-electron concentrationc, the magnetic field and temperature. A quantitative interpretation is given using a Jahn-Teller type Hamiltonian with only one phenomenological coupling constant.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of nutrition 29 (1990), S. 295-298 
    ISSN: 1436-6215
    Keywords: phosphopeptides ; calcium utilization ; minipig ; rat ; vitamin-D-deficiency ; Phosphopeptide ; Kalzium-Utilisation ; Minischwein ; Ratte ; Vitamin-D-Mangel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1436-6215
    Keywords: Ovariectomy ; pig ; bone histology ; calcitriol ; parathyroid hormone ; alkaline phosphatase ; Ovariektomie ; Schwein ; Knochenhistologie ; Calcitriol ; Parathormon ; alkalische Phosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung In der vorliegenden Untersuchung sollte die Eignung des ovariektomierten Schweins als Modell für die postmenopausale Osteoporose beschrieben werden. Hierzu wurde der Einfluß der Ovariektomie (OVX) auf Plasmaparameter des Knochenstoffwechsels und auf knochenhistologische Parameter bei zwei Gruppen von Sauen (9 Monate alt, Nullipara oder 35 Monate alt, Multipara) untersucht. Eine Standarddiät für Schweine mit einem Calciumgehalt von 1,5 % wurde verabreicht. Gesamt-und knochenspezifische alkalische Phosphatase (APt, APb) Calcidiol, Calcitriol und Parathormon (PTH) wurden im Plasma 4, 8, 12 und 18 Monate nach OVX bestimmt. Der Mineralgehalt sowie histologische Parameter wurden in repräsentativen Knochenproben 12 Monate nach OVX und am Ende des Versuchs 18 Monate nach OVX gemessen. Bei jungen Sauen führte die Ovariektomie zu keinen signifikanten Veränderungen der Plasmaparameter mit Ausnahme höherer Konzentrationen des Calcitriols 4 Monate nach OVX. Es wurden weder 12 noch 20 Monate nach OVX signifikante Unterschiede zwischen OVX- und Kontrolltieren in den Parametern der chemischen oder histologischen Knochenanalyse beobachtet. Bei multiparen Sauen waren die Plasmakonzentrationen des PTH gegenüber den Kontrolltieren 8 Monate nach OVX signifikant erhöht. Die Konzentrationen des Calcitriols sowie die Aktivität der APt und APb lagen 12 Monate nach OVX signifikant über den Werten der intakten Tiere. Alle Effekte waren moderat. Weder 12 Monate noch 20 Monate nach OVX kam es zu signifikanten Veränderungen in den Parametern der chemischen oder histologischen Knochenanalyse. Obwohl die beobachteten Veränderungen in den klinisch-chemischen Plasmaparametern zweifellos keine histomorphometrischen Anzeichen einer Osteopenie/Osteoporose zur Folge hatten, bleibt es zukünftigen Untersuchungen überlassen nachzuprüfen, ob das Ausbleiben des Osteopenie ein Ergebnis der in diesem Experiment hohen Calciumzufuhr war. Eine solche Möglichkeit sollte aufgrund der Beobachtung in Betracht gezogen werden, daß Osteopenie beim Schwein nach OVX eintritt, wenn eine Diät mit geringerer Calciumzufuhr (0,75 % im Vergleich zu 0,9 %) verabreicht wurde (33).
    Notes: Summary To investigate the suitability of the pig as animal model for postmenopausal osteoprosis, effects of ovariectomy (OVX) on bone metabolism and histology were studied in two groups of sows (9 months, nulliparous or 35 months, multiparous). A standard diet of about 1.5 % calcium (Ca) was fed till sacrifice at either 12 or 20 months post OVX when mineral content and histology were studied in representative bone specimens of proximal tibia, iliac crest and lumbar vertebrae. At 4, 8, 12, and 18 months post OVX, total and bone-specific alkaline phosphatase (APt, APb) calcidiol, calcitriol and parathyroid hormone (PTH) were measured in plasma. In young sows OVX did not significantly affect plasma variables except for calcitriol, which was higher at 4 months post OVX. No significant differences between OVX or control animals were observed in the variables of bone chemical and histological analyses, neither 12 nor 20 months post OVX. In multiparous sows OVX significantly increased PTH plasma concentrations at 8 months post OVX and plasma calcitriol, APt and APb at 12 months post OVX. All effects were moderate and transient. OVX did not significantly affect the variables of bone chemical and histological analyses neither 12 nor 20 months post OVX. Although undoubtedly the clinical-chemical changes observed were not accompanied by any histomorphometric signs of osteopenia/osteoporosis, it must be left to future experiments as to whether this resulted from the ample calcium supply provided. This possibility is supported by recent observations showing that porcine osteopenia could be induced by OVX in animals maintained on only 0.75 % dietary calcium but not on higher (0.9 %) Ca regimens (33).
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0006-3592
    Keywords: Klebsiella pneumoniae ; glycerol ; pyruvate kinase ; pyruvate:formate-lyase ; pyruvate dehydrogenase ; in vitro and in vivo activities ; dynamics ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The activities of pyruvate kinase (PK), pyruvate: formate-lyase (PFL), pyruvate dehydrogenase (PDH), and citrate synthase (CS) involved in the anaerobic glycerol conversion by Klebsiella pneumoniae were studied in continuous culture under conditions of steady states and sustained oscillations. Both the in vitro and in vivo activities of PK, PFL, and PDH are strongly affected by the substrate concentration and its uptake rate, as is the in vitro activity of CS. The flux from phosphoenolpyruvate to pyruvate is found to be mainly regulated on a genetic level by the synthesis rate of PK, particularly at low substrate concentration and low growth rate. In contrast, the conversion of pyruvate to acetyl-CoA is mainly regulated on a metabolic level by the in vivo activities of PFL and PDH. The ratio of in vitro to in vivo activities is in the range of 1 to 1.5 for PK, 5 to 17 for PFL and 5 to 80 for PDH under the experimental conditions. The regulation of in vivo activity and synthesis of these enzymes is sensitive to fluctuations of culture conditions, leading to oscillations of both the in vitro and in vivo activities. In particular, PFL is strongly affected during oscillations; its average in vitro activity is only about half of its corresponding steady-state value under similar environmental conditions. The average in vitro activities of PDH and PK under oscillations are close to their corresponding steady-state values. In contrast to all other enzymes measured for the glycerol metabolism by K. pneumoniae PFL and PDH are more effectively in vivo utilized under oscillations than under steady state, underlining the peculiar role of pyruvate metabolism in the dynamic responses of the culture. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 617-626, 1998.
    Additional Material: 11 Ill.
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  • 10
    ISSN: 0006-3592
    Keywords: Klebsiella pneumoniae ; glycerol dissimilation ; 1,3-propanediol ; in vitro and in vivo enzyme activities ; dynamics ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The initial steps of glycerol dissimilation and 1,3-propanediol (1,3-PD) formation by Klebsiella pneumoniae anaerobically grown on glycerol were studied by quantifying the in vitro and in vivo activities of enzymes in continuous culture under conditions of steady state and oscillation and during transient phases. The enzymes studied included glycerol dehydrogenase (GDH), glycerol dehydratase (GDHt), and 1,3-propanediol oxidoreductase (PDOR). Three conclusions can be drawn from the steady-state results. First, glycerol concentration in the culture is a key parameter that inversely affects the in vitro activities (concentrations) of all three enzymes, but has a positive effect on their in vivo activities. Growth rate significantly affects the ratio of in vitro and in vivo enzyme activities under low glycerol concentrations, but not under glycerol excess. Second, whereas the flux through the oxidative pathway of glycerol dissimilation is governed mainly by the regulation of in vivo enzyme activity on a metabolic level, the flux through the reductive pathway is largely controlled by the synthesis of enzymes. Third, GDHt is a major rate-liming enzyme for the consumption of glycerol and the formation of 1,3-PD in K. pneumoniae at high glycerol concentrations. Results from oscillating cultures revealed that both in vitro and in vivo activities of the enzymes oscillated. The average values of the in vitro activities during an oscillation cycle agreed well with their corresponding values for nonoscillating cultures under similar environmental conditions. Experiments with step changes in the feed concentration of glycerol demonstrated that growth and product formation are very sensitive to changes of substrate concentration in the culture. This sensitivity is due to the dynamic responses of the genetic and metabolic networks. They should be considered when modeling the dynamics of the culture and attempting to improve the formation of 1,3-PD. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59: 544-552, 1998.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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