ISSN:
1420-9071
Keywords:
K562
;
ɛ-globin mRNA
;
Hb Gower 1
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Long-term cultures of K562(S) cells in 50–75 μM hemin allow the selection of ‘hemin-resistant’ K562 cells together with cells which proliferate efficiently while fully induced to express the human embryonic globin genes, as the hemoglobin Gower 1 (ζ2ε2) is the predominant hemoglobin produced. Our experiments demonstrate that these K562 cells accumulate mostly ɛ-globin mRNA (ɛ-globin mRNA/γ-globin mRNA=2.9) suggesting that the control of hemoglobin expression is at a pretranslational level.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02007714
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