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  • 1
    Electronic Resource
    Electronic Resource
    Postpolio syndrome: poliovirus persistence is involved in the pathogenesis (1999)
    Springer
    Journal of neurology 246 (1999), S. 472-476 
    Details
    ISSN: 1432-1459
    Keywords: Key words Postpolio syndrome ; Reverse transcriptase ; polymerase ; chain reaction ; Poliovirus ; persistence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The pathogenesis of the postpolio syndrome (PPS) remains unclear. In this study we looked for poliovirus (PV) persistence in the CSF of 20 patients with PPS, in a control group including 20 patients with unrelated neurological diseases, and in 7 patients with stable poliomyelitis sequelae. CSF samples and sera were examined using reverse transcriptase–polymerase chain reaction (RT-PCR) for the detection of PV or other enterovirus genomes; this assay allows the detection from as little as 1 fg viral RNA. Sequencing of amplified products from 5 patients was performed. PV genomic sequences were detected in the CSF of 11 of 20 patients with PPS and in none of the control group. Sequencing in the 5′ untranslated region confirmed the presence of mutated PV sequences. These findings suggest that PPS is related to the persistence of PV in the central nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004150050386
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of pandemic viral strains (1994)
    Springer
    European journal of epidemiology 10 (1994), S. 463-464 
    Details
    ISSN: 1573-7284
    Keywords: Identification ; Pandemic ; Surveillance network ; Virus isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The identification of a pandemic viral strain will be performed by the National Reference Centres. The well established sentinels network collecting respiratory samples is able to work not only during the winter season but also a part from it and to allow the early detection of new variants. It also supplies pertinent clinical and epidemiological data in order to correlate a new strain with authentic pathogenic and epidemiologic potential. Based on the previous pandemics experiences, and on the current circulating Influenza strains in humans and animals, a limited number of H and N antigens could be expected as component of a new pandemic strain. The recent rapid tests for the detection and the isolation of influenza viruses permit the diagnosis of a new strain within a few days or weeks. Rapid exchanges of data, strains and reagents under the WHO authority would help in a rapid prediction of a pandemic risk.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01719676
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Role of the French drug licensing authority in the prevention of influenza pandemics (1994)
    Springer
    European journal of epidemiology 10 (1994), S. 507-508 
    Details
    ISSN: 1573-7284
    Keywords: Licensing ; Pandemic influenza ; Vaccines control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The French Drug Agency is responsible for the control and delivery of batch release certificates. In the case of an influenza pandemic, the use of inactivated vaccines, produced according to well-established procedures and controlled according to the European Pharmacopea and FDA requirements, will be strictly dependent on the necessary delays for production and controls. Mutual recognition between the National Control Laboratories in Europe might help in shortening the delays. If new, inactivated vaccines are produced either on cell cultures or by using genetically modified organisms, and if live attenuated vaccines are needed, it would be suitable to organize ad hoc working groups and international collaborative studies in fields of both research and regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01719689
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Recommendations of the 7th European Meeting of Influenza and Its Prevention (1994)
    Springer
    European journal of epidemiology 10 (1994), S. 525-526 
    Details
    ISSN: 1573-7284
    Keywords: Influenza ; Pandemic ; Recommendations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01719696
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Selective inactivation of hemagglutinin and neuraminidase on mumps virus (1979)
    Springer
    Archives of virology 61 (1979), S. 273-281 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The thermal stability and the effect of guanidine on the hemagglutinin and neuraminidase of three strains of mumps virus were compared. The heat inactivation of hemagglutinin resulted in the concomitant loss of neuraminidase. The effect of guanidine at various molarities showed that the neuraminidase was more sensitive than the hemagglutinin and a selective inactivation was obtained after exposure to 1.5m guanidine. However differences in sensitivity of both activities (hemagglutinin and neuraminidase) to heat and guanidine inactivations were observed among strains and correlated with differential susceptibility to non-specific inhibitors of the strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315013
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Correlation between the reactivity patterns of monoclonal antibodies to distinct antigenic sites on HN glycoprotein and their protective abilities in Sendai (6/94) virus infection (1990)
    Springer
    Archives of virology 110 (1990), S. 179-193 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The relative importance of the host immune response to various antigenic and functional sites on the HN glycoprotein of Sendai (6/94) virus for protection in vivo, was evaluated in mice passively immunized with monoclonal antibodies to HN and then intranasally challenged with infectious virus. Five neutralizing monoclonal antibodies reacting with distinct antigenic sites and exhibiting different reactivity patterns were selected. All of them were able to prevent entirely the growth of virus in the lungs of experimental animals injected with appropriate dilutions of monoclonal antibody. The calculation of correlation coefficients between the reduction of virus in the lungs of immunized mice and the amount of antibody, expressed in terms of hemagglutination inhibition, hemolysis inhibition or neutralizing units, showed a high degree of correlation (r=0.89) with neutralization and a lack of correlation (r=0.44) with hemagglutination inhibition. In parallel a minimum threshold value for protection equivalent to 2×103 neutralizing units per mouse was determined independently of the mechanism(s) by which monoclonal antibodies mediated the neutralization of the infectivity. On the HN glycoprotein of Sendai (6/94) virus we could not individualize a critical site for successful immune recognition by antibodies although the characteristics of an “ideal protective monoclonal antibody” have also been defined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311287
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Hybridoma growth in a new generation hollow fibre bioreactor: antibody productivity and consistency (1997)
    Springer
    Cytotechnology 24 (1997), S. 109-119 
    Details
    ISSN: 1573-0778
    Keywords: antibody consistency ; hollow fibre bioreactor ; hybridoma ; monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This paper analyses the performance of MAbMaxTM/TricentricTM, a new generation hollow fibre bioreactor, for hybridoma growth and antibody productivity, the down stream processing of monoclonal antibody harvests throughout the run and the further control of antibody quality consistency. Handling and process parameters were optimised using a mouse hybridoma, IgG1K secretor, and then confirmed with several other hybridomas. Cells were kept at optimal viability during an unusually long period of time and a continuously high production of antibodies was detected over several months. Foetal bovine serum concentration was reduced to 1\% and the effects of weaning of cells from serum were monitored in terms of cell metabolism and antibody productivity. Antibody harvests collected at regular intervals throughout the run (2 to 12 weeks) were purified using affinity chromatography on a recombinant protein A/G matrix and then analysed in terms of antigen binding properties, isoelectric forms and oligosaccharide structures, in order 1) to control antibody quality consistency as a function of time and serum concentration and 2) to compare antibody characteristics as a function of culture conditions, in vitro bioreactor cultivation versus in vivo mouse ascite cultivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007922004714
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    Paper (German National Licenses)
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