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  • 1
    ISSN: 1432-055X
    Keywords: Schlüsselwörter Schock ; Hämorrhagie ; Streßproteine ; Hitzeschockreaktion ; Hämoxygenase ; Key words Haemorrhage ; Shock ; Stress proteins ; Heat shock response ; Haeme oxygenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Objective: Recent evidence suggests a possible role for Haeme oxygenase (HO)-derived carbon monoxide (CO) in the regulation of vascular tone through elevation of cyclic 3′–5′ guanosine monophosphate (cGMP). Previous work from our laboratory has shown that blockade of the HO pathway by tin-protoporphyrin-IX (SnPP) after resuscitation from haemorrhage leads to a specific and profound increase in portal resistance while neither systemic nor hepatic arterial resistance are affected. We therefore investigated the organ-specific expression pattern of the stress-inducible protein haeme oxygenase-1/heat shock protein 32 after haemorrhage and resuscitation. Materials and methods: After approval of the protocol by the local review board, male Sprague-Dawley rats (n=6/group) were anaesthetised with pentobarbitone, instrumented for assessment of central haemodynamics, and subjected to haemorrhagic hypotension (40 mm Hg for 1 h) followed by resuscitation with 60% shed blood and Ringer’s solution or a time-matched sham protocol. Samples of liver, spleen, kidney, intestine, aorta, and lungs were harvested 5 h after the onset of resuscitation and subjected to Western-blot analysis using a specific anti-rat HO-1/hsp 32 antibody (StressGen, Sidney, Canada). Results: Resuscitation with shed blood/Ringer’s solution restored central haemodynamics and acid-base status while significant haemodilution was observed. Haemorrhage and resuscitation led to strong induction of HO-1 in the liver and slight induction in aortic tissue, while no increase in steady-state protein levels was observed in the other organs studied. Conclusion:These results suggest a specific contribution of the HO/CO pathway to maintenance of low hepatic portal resistance in vivo in a clinically relevant model of haemorrhagic shock and adequate resuscitation.
    Notes: Zusammenfassung Kohlenmonoxid (CO) entsteht als Intermediärstoffwechselprodukt im Hämabbau und kann nach neuesten Erkenntnissen ähnlich wie Stickstoffmonoxid über Aktivierung der Guanylatzyklase vasodilatorische Wirkungen entfalten. Für die endogene Bildung von CO ist ausschließlich die mikrosomale Hämoxygenase (HO) verantwortlich, die als konstitutives Enzym (HO-2) sowie in einer streßinduzierbaren Isoform (HO-1 oder Hitzeschockprotein 32) vorliegt. Hemmung des Hämstoffwechsels nach Schock und Volumentherapie führt nach eigenen Untersuchungen zu einer spezifischen Zunahme des Pfortaderwiderstands, ohne vasokonstriktorische Wirkungen in der systemischen Zirkulation hervorzurufen. In der vorliegenden Arbeit wurde daher das organspezifische Expressionsmuster der streßinduzierbaren Isoform des Enzyms nach hämorrhagischem Schock und Volumentherapie untersucht. Methoden und Ergebnisse: In einem kliniknah therapierten Schockmodell an der Ratte führte das Schockereignis zu einer geringfügigen Expression von HO-1 in Aortengewebe sowie zu einer ausgeprägten Expression in der Leber, während eine Induktion in anderen Organen wie Lunge, Darm oder Niere nach Hämorrhagie nicht nachweisbar war. Schlußfolgerung: Diese Ergebnisse legen nahe, daß HO-1/Hitzeschockprotein 32 in der Leber ein streßinduzierbares Vasodilatatorsystem, das zur Aufrechterhaltung der Leberperfusion nach hämorrhagischem Schock beiträgt, darstellt.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Macromolecules 24 (1991), S. 2634-2636 
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 18 (1968), S. 114-122 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1238
    Keywords: Key words Abdominal aortic aneurysm ; Cytokines ; Systemic inflammatory response syndrome ; Ischemia-reperfusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective: To characterize the impact of abdominal aortic aneurysm repair (AAAR) on spontaneous as well as lipopolysaccharide (LPS)-induced gene expression of pro- and anti-inflammatory cytokines. Design: Prospective, controlled in vivo / ex vivo study. Setting: University hospital. Patients and interventions: Whole blood from 14 consecutive patients undergoing AAAR withdrawn prior to surgery (T1), at the end of ischemia (T2), 90 min after declamping (T3) and on the first postoperative day (T4) was cultured in the absence or presence of LPS. Five patients undergoing elective inguinal hernia repair served as controls. Measurements and results: While tumor necrosis factor (TNF), Interleukin (IL)-1 and IL-10 plasma concentrations did not increase significantly, IL-6 was elevated at each time point, as compared with T1. Despite the spontaneous release of trace amounts of IL-6, the ability of cultured whole blood to mount a cytokine response in vitro to LPS was impaired for all cytokines studied at T2 (TNF –62 %, IL-1 –51 %, IL-6 –20 %, IL-10 –51 %). The stimulated IL-6 response was restored early after declamping (T3: + 56 %) and enhanced 1 day after operation (T4: + 144 %). In contrast, stimulated TNF and IL-1 responses remained depressed at T3 (TNF –48 %, IL-1 –64 %) and T4 (TNF –40 %, IL-1 –24 %). A biphasic pattern was observed for IL-10 with initial depression at T3 (-51 %) and restoration at T4 ( + 40 %). Among the different cytokines monitored, only impaired TNF responsiveness at early reperfusion (T3) correlated with the postoperative course, as reflected by APACHE II. Cytokine response to LPS was maintained or even increased during and after surgery in the whole blood from patients undergoing hernia repair. Conclusions: Despite consistent development of clinical signs of systemic inflammatory response syndrome (SIRS) and spontaneous release of IL-6 abdominal aortic aneurysm repair produces a state of impaired pro-inflammatory cytokine response upon a subsequent in vitro Gram-negative stimulus. This early impairment of TNF responsiveness seems to correlate with an unfavorable postoperative course.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 308-311 (May 1999), p. 113-122 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für Herz-, Thorax- und Gefässchirurgie 14 (2000), S. 204-211 
    ISSN: 0930-9225
    Keywords: Schlüsselwörter Venenentnahme – Wundheilungsstörungen –¶kosmetische Inzision ; Key words Harvesting of the saphenous vein –¶wound healing disturbances – cosmetic incision
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In Germany about 75000 patients received the CABG operation last year, these are about 75% of all open heart operation procedures. The most frequently used conduit is the greater saphenous vein. In the last years a lot of new minimally invasive techniques for vein harvesting revolutionized the field of CABG operation procedures, nevertheless the most common technique today is the traditional, open technique with long, continuous incision for the open preparation of the saphenous vein. Using traditional open saphenectomy, wound healing disturbances are common and have been reported to occur in 5–25% of patients. The prevalence of wound healing disturbances varies widely, depending to some degree of definition, as well of the intensity of follow up. Minimal and less invasive vein harvesting studies have been shown to be effective in decreasing leg wound disturbances, resulting much superior cosmetic results. Advantages and disadvantages of minimally invasive vein harvesting techniques are today the topic of controversy discussions in heart surgery.¶   We reported our experience of modified minimally invasive harvesting of the saphenous vein using the Aesculap retractor system with performing 3–4 small cutaneous incisions. According to our experience these results show that the use of modified minimally invasive harvesting of the great saphenous vein is an attractive alternative to the traditional open harvest technique with fewer wound complication, more patient satisfaction and excellent cosmetic aspects. Therefore, we hope that less or minimally invasive systems for harvesting of the saphenous vein will be established in the future in CABG operation procedures.
    Notes: Zusammenfassung Jährlich werden in Deutschland ca. 75000 Patienten mit koronarer Herzerkrankung (ca. 75% aller Herzoperationen) operativ versorgt. Als Bypassmaterial stehen arterielle Grafts und autologe Venen, vorwiegend die Vena saphena magna, zur Verfügung. In den letzten Jahren revolutionieren minimal-invasive Venenentnahmetechniken die Bypasschirurgie, dennoch erfolgt heute die Saphenektomie zum Großteil mittels kontinuierlicher Inzision der Haut vom Knöchel bis in die Leiste zur offenen Präparation der Vena saphena magna. Wundheilungsstörungen nach offener Saphenektomie werden wiederholt diskutiert und sind Gegenstand jeder herzchirurgischen Nachbehandlung nach koronarchirurgischen Eingriffen und Grund für die Aufwertung neuer, minimal-invasiver Entnahmetechniken. Diese Wundheilungsstörungen, je nach Beobachtungszeitraum, werden in der Literatur zwischen 5–25% angegeben. Die Vor- und Nachteile minimal-invasiver Systeme sind derzeit Inhalt kontroverser Diskussion.¶   Die vorgestellte Technik (modifizierte „Bridge”-Technik) zeigt erwünschte Ergebnisse, insbesondere eine sehr hohe Patientenzufriedenheit hinsichtlich des kosmetischen Ergebnisses und der nur geringen Einschränkung durch die Saphenektomiewunde(n). Es ließ sich nur eine geringe Rate an postoperativen Komplikationen (hier 3%) feststellen. Minimal-invasive Venenentnahmesysteme halten wir für praktikabel, effizient und leicht erlernbar. Wir sehen es deshalb als wichtig an, diese Techniken weiter im klinischen Gebrauch zu etablieren.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0006-291X
    Keywords: [abr] (ADPR)"n; polyadenosine diphosphoribose ; [abr] 5-IABP; 5-iodo-6-amino-1,2-benzopyrone ; [abr] 6-ABP; 6-amino-1,2-benzopyrone ; [abr] ADPRT; adenosine diphosphoribose transferase ; [abr] HIV; human immunodeficiency virus ; [abr] MNNG; 1-methyl-3-nitro-l-nitrosoguanidine ; [abr] RT; reverse transcriptase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Sensors and Actuators A: Physical 37-38 (1993), S. 497-501 
    ISSN: 0924-4247
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0009-8981
    Keywords: Captopril ; Cellular proliferation ; Potassium (^8^6Rb)-influx
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0006-291X
    Keywords: [abr] (ADPR)"n; polyadenosine diphosphoribose ; [abr] 5-IABP; 5-iodo-6-amino-1,2-benzopyrone ; [abr] 6-ABP; 6-amino-1,2-benzopyrone ; [abr] ADPRT; adenosine diphosphoribose transferase ; [abr] HIV; human immunodeficiency virus ; [abr] MNNG; 1-methyl-3-nitro-l-nitrosoguanidine ; [abr] RT; reverse transcriptase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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