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  • 1
    ISSN: 1432-0428
    Keywords: Key words Diabetic glomerulopathy, microalbuminuria, basement membrane thickness, mesangial expansion, mesangial matrix, stereology, hyperglycaemia.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We investigated in a randomized, prospective study the influence of improved blood glucose control during 2–3 years in young insulin-dependent diabetic (IDDM) patients with microalbuminuria, which is indicative of early nephropathy. Patients were randomized either to intensive treatment by continuous subcutaneous insulin infusion (CSII) (n =9) or CT (n =9). Kidney biopsies were taken at baseline and after 26–34 months. End points were structural changes in the glomeruli. Sensitive, quantitative, morphometric methods were used. The blood glucose control improved significantly (p =0.01) during the study in the CSII-group as glycated haemoglobin (HbA1 c) fell from 10.1 % ([95 % CI] 8.9–11.3) to 8.6 % (7.9–9.2), but not in the CT-group, 10.1 % (8.3–11.9) vs 9.7 % (8.7–10.8). Mean HbA1 c during the study period was significantly lower in the CSII-group than in the CT-group, 8.7 % (8.1–9.3) vs 9.9 % (8.5–11.3), p =0.04. Basement membrane thickness (BMT) increased in both groups, most (CT vs CSII, p =0.03) in the CT-group: 140 nm (50–230) vs CSII: 56 nm (27–86). In the CT-group only an increase was seen in matrix/mesangial volume fraction (p =0.006) and matrix star volume (p =0.04). Furthermore, a positive correlation between mean HbA1 c during the study and change from baseline in BMT (r =0.70, p =0.001) and matrix/glomerular volume fraction (r =0.33, p =0.09, NS) was demonstrated. Albumin excretion rate correlated significantly to BMT and most of the matrix parameters. The present study shows that during a period of only 2.5 years, a close relationship between the level of mean blood glucose and progression of glomerular morphological changes in early diabetic nephropathy can be demonstrated. [Diabetologia (1994) 37: 483–490]
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  • 2
    ISSN: 1432-0428
    Keywords: Combined Pancreas ; Kidney ; Transplantation Glucose ; Metabolism Long-term
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Metabolic glucose control was followed in 36 patients at 12-month intervals for up to 5 years after a successful combined kidney and segmental duct-occluded pancreas transplantation. All recipients had normal blood glucose levels at each examination. HbA1 values, intravenous glucose tolerance test, C-peptide levels and C-peptide responses to glucagon stimulation were also, on average, within the normal range. Several individual patients had, however, abnormal values for these parameters. At most 46% had abnormal values for HbA1 and intravenous glucose tolerance test, up to 13% showed low C-peptide values and up to 46% of the stimulated C-peptide responses were inadequate at the different intervals. These parameters did not deteriorate with time. This was true both for the whole group of patients as well as for the 6 patients with a 5-year observation time evaluated separately. Despite these abnormalities in glucose metabolism, all patients remained normoglycaemic without need for exogenous insulin up to 5 years after transplantation. The long-term ability of duct-occluded segmental pancreatic grafts to preserve euglycaemia therefore seems to remain intact at least for 5 years.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Der Anaesthesist 46 (1997), S. 890-894 
    ISSN: 1432-055X
    Keywords: Schlüsselwörter Lungenembolie ; Prolongierte kardiopulmonale Reanimation ; Medikamentöse Thrombolyse ; Key words Pulmonary embolism ; Prolonged cardiopulmonary resuscitation ; Thrombolytic therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract A healthy 38-year-old woman suffered a sudden cardiac arrest 2 days after a vaginal hysterectomy. Although standard cardiac life support (CPR) was instituted immediately after the event, it was not possible to re-establish a spontaneous circulation for about 40 min. Systemic intravenous thrombolytic therapy with slow injection of 1.5 million IU urokinase was performed as a final life-maintaining measure because of the high probability that the underlying cause was a pulmonary embolus; 10 min later (after 60 min of ongoing CPR) the patient regained a stable circulation. She survived without neurological deficit in spite of the long duration of CPR.
    Notes: Zusammenfassung Eine 48jährige Frau ohne Vorerkrankungen erlitt 2 Tage nach einer komplikationslos verlaufenen vaginalen Hysterektomie einen plötzlichen Herz-Kreislaufstillstand. Durch die üblichen Maßnahmen der kardiopulmonalen Reanimation über einen Zeitraum von etwa 40 min konnte kein Spontankreislauf wiederhergestellt werden. Bei dringendem Verdacht auf eine postoperative Lungenembolie entschlossen sich die behandelnden Ärzte zum Versuch einer systemischen Thrombolyse. 1,5 Mio I.E. Urokinase wurden unter kardiopulmonaler Reanimation intravenös injiziert. Etwa 10 min nach Gabe des Fibrinolytikums konnte der Spontankreislauf der Patientin reetabliert werden. Trotz der langen Dauer der Wiederbelebungsmaßnahmen von insgesamt 60 min überlebte die Patientin ohne neurologisches Defizit.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-055X
    Keywords: Schlüsselwörter Transportable Kapnometer ; Rettungsdienst ; Genauigkeit ; Key words Portable capnometers ; Emergency medicine ; Accuracy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Capnometry, the noninvasive measurement of end-expiratory CO2 concentration (cCO2, vol%) or calculation of its respective partial pressure (pCO2; mmHg) is an established method. However, for prehospital settings, capnometry is still used very restrictively, mainly owing to the respective devices used. The prerequisite for their use is sufficient accuracy (±2 mmHg) and easy handling. Two special capnometers (STAT CAP, Nellcor: mainstream, semiquantitative estimation; Capnocheck 8200, BCI: sidestream, quantitative measurement, numeric display), developed recently for potential use in emergency medicine, are said to fit these criteria. Therefore, the objective of the present investigation was to assess the accuracy and precision of both devices, comparing methods under standardized in vitro (reference gases) and in vivo (intubated and ventilated patients) conditions. Methods. Both devices (“STAT CAP”: pCO2 range, light bars; “Capnocheck 8200”) were evaluated regarding the accuracy of pCO2 (Capnocheck) and the precision of the CO2 range (STAT CAP). Tests were performed with four dry gas mixtures (STPD) of defined composition and during ventilation of 20 intubated patients (BTPS). All measurements were compared with the alveolar gas monitor “AGM 1304” (Brüel & Kjaer, Denmark) as a reference method with a proven ±1 mmHg accuracy of pCO2 measurement. Results. The “Capnocheck” (BCI) presented an accuracy of the pregiven pCO2 of 0.7–1.4 mmHg (dry gas mixtures, STPD) and an overestimation of 0.2±4.1 mmHg (BTPS) during ventilation with pure oxygen; inaccuracy during ventilation with 70% N2O in O2 proved to be +1.2±1.7 mmHg (BTPS). Nellcor's “STAT CAP” failed to reach the target value in 10% of analyses, as shown by the respective segment bar of the display. Conclusion. Evaluation of the accuracy of capnometers must focus on the necessary pH2O correction and the possible effects exercised by O2 (and N2O) as well as the possible dependence on barometric pressure (if pCO2, mmHg, is the desired value). The “Capnocheck” showed an accuracy of more than 2 mmHg in dry gas mixtures as well as in humidified air. Concerning the practical use during constant artificial ventilation, the digital display and accuracy of the sidestream capnometer allow for reliable conclusions on patients' ventilation and circulation (CO2 elimination). The 90% accuracy of the segment bar display of Nellcor's “STAT CAP”, per se covering only a rather broad range of 20 mmHg, obviously does not provide more than a rough overview. Therefore, the STAT CAP cannot be recommended for prehospital capnometry in the field. However, both the accuracy of the BCI capnometer (Capnocheck) and its numeric display and easy handling strongly recommend this device also for clinical use.
    Notes: Zusammenfassung Ziel vorliegender Untersuchung war die Prüfung der CO 2 -Meßgenauigkeit bzw. -Meßbereichspräzision zweier neuer, potentiell für den Rettungsdienst konzipierter Kapnometer unterschiedlicher Funktionsweise im Rahmen eines Methodenvergleichs. Untersucht wurden das Hauptstrom- (STAT CAP, Nellcor: Balkensegmentanzeige, pCO 2 -Bereiche) und das Nebenstromkapnometer (Capnocheck 8200, BCI International: pCO 2 -Wertanzeige). Geprüft wurde mit vier Referenzgasen und an 20 beatmeten Patienten. Als Referenzmethode diente der Atemgasmonitor „AGM 1304“ (Brüel & Kjaer) mit einer pCO 2 -Meßgenauigkeit von ±1 mm Hg. Die Abweichungen des „Capnocheck“ vom Sollwert betrugen für die Prüfgase 0,7–1,4 mm Hg, bei Beatmung mit reinem O 2 0,2±4,1 mm Hg (unter N 2 O 1,2–1,7 mm Hg). Der „STAT CAP“ traf den vorgegebenen Sollwert nur in 90% mit dem richtigen Segment seiner Bereichsanzeige. Der „Capnocheck“ erreichte sowohl in trockenen Prüfgasen (STPD) als auch in wasserdampfgesättigter Ausatemluft (BTPS) eine pCO 2 -Meßgenauigkeit von ≤±2 mm Hg. Die Bereichsanzeige des „STAT CAP“ erlaubt aufgrund der Unschärfe des pCO 2 -Bereichs von 20 mm Hg sowie wegen des 10%igen Trefffehlers lediglich eine grobe Orientierung. Dieses Gerät (Preis ca. DM 2500) ist zur Verwendung als Kapnometer im eigentlichen Sinne untauglich, könnte jedoch zur „vor-Ort-Beurteilung“ der Tubuslage Bedeutung erlangen. Die Meßgenauigkeit und gute Handhabbarkeit des „Capnocheck“ empfehlen dieses Kapnometer (Preis ca. DM 4500) sowohl für den potentiellen Einsatz bei beatmeten Patienten im Rettungsdienst als auch für den innerklinischen Betrieb.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0738
    Keywords: Industrial compounds ; PCP ; 2,4-DB ; Dinoseb ; Bromoxynil ; Thyroid hormone carriers ; Transthyretin ; Plasma TT4 ; Plasma TT3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Previous results in experimental systems have suggested that hydroxylated PCBs may decrease thyroid hormone levels through associative interaction with transthyretin. In the present paper it was investigated whether this property was also shared by various industrial chemicals, mainly pesticides. In total, 65 compounds from 12 chemical groups were analyzed for direct interference with the T4 binding site of transthyretin using a competitive binding assay. Sixty per cent of the compounds were competitive at a concentration level of 100 μM. Relatively strong interactions were observed by several chlorophenols, chlorophenoxy acids and nitrophenols, as well as by individual compounds such as hexachlorobenzene, dicofol, bromoxynil and tetrachlorohydroquinone. Examples from these chemical groups, e.g. pentachlorophenol, 2,4-dichlorophenoxybutyric acid, dinoseb and bromoxynil, also reduced plasma TT4 levels in rats. In addition, bromoxynil decreased plasma TT3 levels. The results suggest the existence of a number of halogenated industrial chemicals with a potential for lowering plasma thyroid hormone levels through interference with hormone transport carriers.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 160 (1993), S. 432-439 
    ISSN: 1432-072X
    Keywords: Nitrous oxide reduction ; Dinitrogen formation ; Denitrification ; NOx-production ; Nitrate dissimilation ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Escherichia coli K12 reduces nitrous oxide stoichiometrically to molecular nitrogen with rates of 1.9 μmol/h x mg protein. The activity is induced by anaerobiosis and nitrate. N2+formation from N2O is inhibited by C2H2 (K i ∼ 0.03 mM in the medium) and nitrite (K i=0.3 mM) but not by azide. A mutant defective in FNR synthesis is unable to reduce N2O to N2. The reaction in the wild type could routinely be followed by gas chromatography and alternatively by mass spectrometry measuring the formation of 15N2 from 15N2O. The enzyme catalyzing N2O-reduction in E. coli could not be identified; it is probably neither nitrate reductase nor nitrogenase. E. coli does not grow with N2O as sole respiratory electron acceptor. N2O-reduction might not have a physiological role in E. coli, and the enzyme involved might catalyze something else in nature, as it has a low affinity for the substrate N2O (apparent K m∼3.0 mM). The capability for N2O-reduction to N2 is not restricted to E. coli but is also demonstrable in Yersinia kristensenii and Buttiauxella agrestis of the Enterobacteriaceae. E. coli is able to produce NO and N2O from nitrite by nitrate reductase, depending on the assay conditions. In such experiments NO inf2 sup- is not reduced to N2 because of the high demand for N2O of N2O-reduction and the inhibitory effect of NO inf2 sup- on this reaction.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1084
    Keywords: Contrast media ; Comparative study ; Intravenous digital subtraction angiography ; Iodixanol ; Iopamidol ; Kidney function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Iodixanol (visipaque, 320 mgI/ml) was compared with iopamidol (Solutrast, 370 mfI/ml) in a double-blind, randomized, parallel group, intravenous DSA phase-III trial for evaluation of safety and efficacy. A total of 117 patients received iodixanol (n = 60) or iopamidol (n = 57). Diagnostic efficacy was evaluated using categoric and visual analogue scales. Discomfort and adverse evenets were recorded. A total of 39 patients collected urine up to 72 h after the examination for analysis. Diagnostic efficacy and radiographic density were similar in both groups. Discomfort was milder with iodixanol. The difference between the frequency of adverse events between both groups (iodixanol = 7, iopamidol = 2) was without statistical significance. Creatinine clearance was slightly more affected by iodixanol, whereas the increase in renal excretion ofN-acetyl-beta-glucosaminidase (NAG) in the first 24 h collection period after the examination was significantly higher (p 〈 0.01) with iopamidol. Iodixanol was of equal diagnostic efficacy compared with iopamidol despite its reduced iodine content. Both contrast media are well suited for IV DSA.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1084
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Iodixanol (Visipaque, Nycomed Imaging AS, Oslo, Norway), an isotomic, dimeric and non-ionic contrast medium (CM), and iohexol (Omnipaque, Nycomed Imaging AS, Oslo, Norway), a low-osmolar, monomeric and non-ionic contrast medium, were used as glomerular filtration rate (GFR) markers in patients with severely impaired renal function. Different methods for determining GFR were compared. A total of 16 patients with s-creatinine 〉 400 μmol/l were enrolled in the study; 8 in each CM group. Serum-iodine was measured, and plasma CM clearance was determined using the Bröchner-Mortensen method, the single-sample method and conventional method. The ratios between the results obtained from the conventional method and each of the two other methods were calculated. These data were plotted against the mean of the pairs compared, and the upper and lower limits of agreement were calculated as the mean ratio ± 2SD. The comparison showed a high degree of agreement between methods, and the two simpler methods seem to be good alternatives to the conventional method, which gave good estimates of GFR (vs that determined by means of renal 125I-iothalamate clearance) when 24-h blood samples were included. However, slight overestimations of GFR, due to extrarenal excretion of the CM, were observed. In summary, serum clearance of iodixanol, as determined by the Bröchner-Mortensen method or single-sample method seems to be a simple and accurate marker of GFR in patients with severely reduced renal function. The findings obtained with iodixanol were similar to those obtained with iohexol.
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  • 9
    ISSN: 1432-1041
    Keywords: Chlorthalidone ; spironolactone ; hypertension ; potassium deficiency ; diuretic ; Kühns' test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Kühns' oral test for potassium deficiency (KDT) has been used to evaluate potassium balance after treatment with diuretics. The test was first standardised in 23 patients, both with and without known potassium deficiency. Subsequently, 18 patients with essential hypertension were investigated before and after the use of 3 different diuretics, each for a period of 6 weeks; all patients received all three forms of treatment but in different sequence. Before treatment the patients excreted an average of 121 mEq potassium a day in urine after oral administration of potassium 127 mEq (citrate). After treatment with chlorthalidone (Hygroton®), 50 mg a day, the excretion of potassium decreased significantly to 83 mEq, suggesting an intracellular deficit of it. Treatment with chlorthalidone 50 mg and potassium chloride 2 g daily (Hygroton — K®), led to potassium excretion of 100 mEq after loading, which was distinctly, but not significantly, larger than in the period of treatment with chlorthalidone alone. After chlorthalidone 50 mg daily and spironolactone (Aldactone “Searle”®), 25 mg q. i. d., the excretion of potassium was 121 mEq, which was the same as before treatment. The mean serum potassium before treatment was 4.4 mEq/l, after chlorthalidone 3.8 mEq/l, after chlorthalidone plus potassium chloride 3.9 mEq/l, after chlorthalidone combined with spironolactone 3.9 mEq/l. The results were in agreement with previously published investigations of “exchangeable potassium” after similar treatments. As the test for potassium deficiency gave better information about the amount of intracellular potassium in the body than analysis of serum potassium, it is suitable for the evaluation of potassium deficiency associated with diuretic therapy.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 9 (1976), S. 265-275 
    ISSN: 1432-1041
    Keywords: Bumetanide ; diuretic ; end-stage renal failure ; proximal tubule ; muscle pain ; side-effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The effect of bumetanide, a new potent diuretic, was studied in twelve patients with severe chronic renal failure (GFR 2.7 – 10.7 ml/min). Bumetanide 8 mg i.v. caused increased excretion of water and sodium in all patients. In some patients sodium excretion was greater than 50 % of filtered load indicating an effect on proximal tubules. Bumetanide 2 mg i.v. was significantly less effective than 8 mg and a greater diuretic effect was produced by bumetanide 16 mg. In a comparative study bumetanide 8 mg was less potent than furosemide 250 mg, a finding in contrast to the potency ratio of 1/40 in other conditions. Side effects consisted of mild to moderate muscle pain and stiffness, especially localized in the neck, shoulders and calves. These side effects occurred only in patients with a GFR less than 5.3 ml/min. They were noted in all patients receiving 16 mg and in 3 out of 12 patients who took bumetanide 8 mg. There was no relationship between the occurrence of side effects and plasma bumetanide levels, electrolyte levels or the renal excretion of bumetanide and electrolytes.
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