Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Experimental dermatology 13 (2004), S. 0 
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The hair follicle offers an exquisite model for the experimental exploration of key issues of cutaneous neuroimmunology, for example, how local, intracutaneous and systemic stress–response systems are integrated with the skin immune system and with epithelial–mesenchymal interactions (as they occur during hair follicle growth and cycling). Previously, we had shown that skin mast cells, which operate as central switchboards of inflammation and tissue remodelling, also are important regulators of hair growth in mice and that endogenous, immunomodulatory mast cell secretagogues are potent hair growth modulators. This is true both for secretagogues that are generated by the hair follicle epithelium itself (e.g. ACTH) and for mast cell-activating neuropeptides synthesized by the sensory hair follicle innervation (e.g. SP). Also, we had shown that the prototypic stress-associated neuropeptide, SP, plays a crucial role in mediating the hair growth-inhibitory, mast cell-activating, inflammation- and catagen-promoting properties of chronic psychoemotional stress on murine hair follicles. Now, we show that the immunomodulatory and mast cell-activating neurotrophin, NGF, is also crucially involved in mediating the inhibitory effects of stress on murine hair growth. Furthermore, the central, stress-related neurohormone CRH, a recognized mast cell secretagogue which is expressed by the hair follicle epithelium, also is a hair growth inhibitor and activates a fully functional peripheral equivalent of the hypothalamic-pituitary-adrenal axis within organ-cultured human scalp hair follicles, including the synthesis and secretion of cortisol as well as the induction of classical feedback loops. We also demonstrate that one of the melanocortins whose intrafollicular synthesis is stimulated by CRH (α-MSH) is a potent suppressor of MHC class I expression in situ and is thus capable of restoring the collapsed immune privilege of human anagen hair bulbs, while SP upregulates the ectopic expression of MHC class I, thus endangering the hair follicle immune privilege. Finally, we show that vanilloids long exploited as experimental tools for neuroimmunological research in the skin (capsaicin) can, in fact, directly modulate human hair growth via the stimulation of vanilloid receptors (VR1) expressed by the follicle epithelium, in addition to stimulating vanilloid expressed by skin mast cells. Therefore, the hair follicle offers an ideal, highly instructive and clinically most relevant research model for dissecting how nervous system, central and peripheral (neuro-) endocrine signalling loops and the immune system interact in order to adapt skin functions to changing environmental conditions (e.g. in response to external stressors, by alterating, e.g. keratinocyte proliferation/apoptosis, skin immune status, as well as defined cutaneous metabolic and endocrine activities).
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: While the enormous clinical and psychosocial importance of pruritus in many areas of medicine and the detrimental effects of chronic ‘itch’ on the quality of life of an affected individual are widely appreciated, the complexity of this sensation is still often grossly underestimated. The current Controversies feature highlights this complexity by portraying pruritus as a truly interdisciplinary problem at the crossroads of neurophysiology, neuroimmunology, neuropharmacology, protease research, internal medicine, and dermatology, which is combated most successfully if one keeps the multilayered nature of ‘itch’ in mind and adopts a holistic treatment approach – beyond the customary, frequently frustrane monotherapy with histamine receptor antagonists. In view of the often unsatisfactory, unidimensional, and altogether rather crude standard instruments for pruritus management that we still tend to use in clinical practice today, an interdisciplinary team of pruritus experts here critically examines recent progress in pruritus research that future itch management must take into consideration. Focusing on new insights into the neuroimmunological, neuroendocrine, and neurophysiological bases of pruritus, and discussing available neuropharmacological tools, specific research avenues are highlighted, whose pursuit promises to lead to novel, and hopefully more effective, forms of pruritus management.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    International Journal of Radiation Applications & Instrumentation. Part D, 17 (1990), S. 587-589 
    ISSN: 1359-0189
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 63 (1988), S. 38-42 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: An experimental system for studying acoustic signals generated by a modulated MeV ion beam in metals is described. For detection, a closed cell on the rear side of a copper or aluminum sample, a half-inch condenser microphone, and a lock-in amplifier were employed. The pressure signal was found to be proportional to beam current and particle energy, and inversely proportional to cell length. Decrease of the signal magnitude and increase of the phase delay with increasing modulation frequency and sample thickness were also observed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Inst. and Methods in Physics Research, B 17 (1986), S. 554-555 
    ISSN: 0168-583X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Instruments and Methods In Physics Research 212 (1983), S. 383-386 
    ISSN: 0167-5087
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Instruments and Methods 175 (1980), S. 60-61 
    ISSN: 0029-554X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Instruments and Methods 112 (1973), S. 399-404 
    ISSN: 0029-554X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Inorganic and Nuclear Chemistry 37 (1975), S. 1583-1585 
    ISSN: 0022-1902
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  Human hair growth can currently be studied in vitro by the use of organ-cultured scalp hair follicles (HFs). However, simplified organotypic systems are needed for dissecting the underlying epithelial–mesenchymal interactions and as screening tools for candidate hair growth-modulatory agents.Objectives  To optimize the design and culture conditions of previously published organotypic systems that imitate epithelial–mesenchymal interactions in the human HF as closely as possible.Materials and methods  Continuous submerged organotypic ‘sandwich’ cultures were established. These consist of a pseudodermis (collagen I mixed with and contracted by human interfollicular dermal fibroblasts) on which one of two upper layers is placed: either a mixture of MatrigelTM basement membrane matrix (BD Biosciences, Bedford, MA, U.S.A.) and follicular dermal papilla fibroblasts (DPC), with outer root sheath keratinocytes (ORSK) layered on the top (‘layered’ system), or a mixture of MatrigelTM, DPC and ORSK (‘mixed’ system). Morphological and functional characteristics of these ‘folliculoid sandwiches’ were then assessed by routine histology, histomorphometry and immunohistochemistry.Results  In both ‘layered’ and ‘mixed’ systems, the ORSK formed spheroid epithelial cell aggregates, which retained their characteristic keratin expression pattern (i.e. cytokeratin 6). In the ‘mixed’ sandwich model the size of the epithelial cell aggregates was smaller, but the numbers of ORSK were significantly higher than in the ‘layered’ model at day 14 in the culture. ORSK proliferated better in the ‘mixed’ than in the ‘layered’ sandwich system, regardless of the calcium or serum content of the media, whereas apoptosis of ORSK was lowest in the ‘mixed’ system in serum-free, low calcium medium. The kinetics of proliferation and apoptosis of DPC, which retained their characteristic expression of versican, were similar in both systems. However, proliferation and apoptosis of DPC were higher in the presence of serum and/or under high calcium conditions.Conclusions  Our results underscore the importance of structural design and medium composition for epithelial–mesenchymal interactions as they occur in the human HF. Specifically, we report a new organotypic submerged ‘folliculoid sandwich’ system with serum-free, low calcium medium and a mixture of interacting human DPC and ORSK, which offers several advantages over previously available assays. This system allows the standardized assessment of the effects of a test agent on the proliferation, apoptosis and key marker expression of human ORSK and DPC under substantially simplified in vitro conditions which approximate the in vivo situation.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...