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Production of insulin-like growth factor I (IGF-I) and IGF-binding proteins by rat intestinal stromal cells in vitro (1998)

MacDonald, R. G. ; McCusker, R. H. ; Blackwood, D. J. ; [et al.]

Springer

Cellular and molecular life sciences 54 (1998), S. 158-166

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ISSN: 1420-9071

Keywords: Key words. Insulin-like growth factors; IGFs; IGFBP; stroma; epithelium; stromal-epithelial interactions.

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract. To determine if intestinal stromal cells secrete diffusible factors such as insulin-like growth factors (IGFs) capable of regulating epithelial cell growth in vitro, stromal cells were isolated by enzymatic digestion of rat intestine. Incorporation of [3H]thymidine into DNA and [14C]leucine into protein of IEC-6 cells, a model intestinal epithelial cell line, was significantly increased (two- to threefold) when the IEC-6 cells were co-cultured with stromal cells, relative to IEC-6 cells grown alone. Medium conditioned by stromal cells stimulated DNA synthesis of IEC-6 cells in a dose-dependent manner. Analysis of the conditioned medium revealed that intestinal stromal cells secreted IGF-I, but little IGF-II, in addition to an M r 32,000 IGF-binding protein (IGFBP-2) and an IGFBP having M r∼ 24,000. We conclude that rat intestinal stromal cells secrete one or more diffusible factors, which may include IGF-I and IGFBPs, capable of stimulating proliferation of IEC-6 cells in vitro.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s000180050137

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Corrosion behaviour of high copper dental amalgams (2004)

Yap, A. U. J. ; Ng, B. L. ; Blackwood, D. J.

Oxford, UK : Blackwell Science Ltd

Journal of oral rehabilitation 31 (2004), S. 0

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ISSN: 1365-2842

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: summary This study evaluated the corrosion behaviour of two high copper dental amalgam alloys [Dispersalloy (Dentsply-Caulk) and Tytin (Kerr)] in different electrolytes. Amalgam specimens were prepared, coupled to a copper wire, cemented into glass tubes and polished to a 600-grit finish. A corrosion cell was prepared using a carbon counter-electrode, a standard calomel electrode as the reference and amalgam as the working electrode. The alloys were tested in the following mediums at 37 °C: (i) artificial saliva based on Fusayama's solution (FS), (ii) artificial saliva with citric acid adjusted to pH 4·0 (FC) and (iii) 1% sodium chloride solution (SC). Corrosion potentials (Ecorr) and corrosion rates (Icorr) were determined using potentiostatic and impedance spectroscopy methods. Data was subjected to anova/Scheffe's post hoc test at 0·05 significance level. For both alloys, the corrosion potential in FS was significantly greater than in SC. Corrosion potential of Tytin in FS and SC was also significantly greater than in FC. The corrosion rate of Dispersalloy in FC was significantly greater than in FS and SC. For Tytin, corrosion rate in SC was significantly greater than in FS and FC. Although no significant difference in corrosion potential/rate was observed between the alloys when tested in FS, significant differences were observed when electrochemical testing was carried out in FC and SC. The corrosion behaviour of high copper amalgam alloys are both material and environment dependent. Certain food substances may increase the corrosion of high copper amalgams.