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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 138 (1984), S. 49-53 
    ISSN: 1432-072X
    Keywords: Lactobacillus plantarum ; Oxygen utilization ; Hydrogen peroxide accumulation ; Acetate accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth rate of Lactobacillus plantarum in a complex medium with 55.6 mM glucose decreased during aerobic incubation (relative to anaerobic incubation). The decrease occurred much earlier than an increase in the rate of oxygen utilization by the culture which led to H2O2 accumulation. The concentration of H2O2 accumulated in the medium was easily tolerated by the culture and elimination of the H2O2 did not prevent the decrease in growth rate. Increased O2 utilization was accompanied by a switch in metabolism which resulted in acetate rather than lactate accumulation in aerobic cultures.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 141 (1985), S. 75-79 
    ISSN: 1432-072X
    Keywords: Lactobacillus plantarum ; Lactate utilization ; Oxygen utilization ; Lactate to acetate pathway
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lactobacillus plantarum P5 grew aerobically in rich media at the expense of lactate; no growth was observed in the absence of aeration. The oxygen-dependent growth was accompanied by the conversion of lactate to acetate which accumulated in the growth medium. Utilization of oxygen with lactate as substrate was observed in buffered suspensions of washed whole cells and in cell-free extracts. A pathway which accounts for the generation of adenosine triphosphate during aerobic metabolism of lactate to acetate via pyruvate and acetyl phosphate is proposed. Each of the enzyme activities involved, nicotinamide adenine dinucleotide independent lactic dehydrogenase, nicotinamide adenine dinucleotide dependent lactic dehydrogenase, pyruvate oxidase, acetate kinase and NADH oxidase were demonstrated in cell-free extracts. The production of pyruvate, acetyl phosphate and acetate was demonstrated using cell-free extracts and cofactors for the enzymes of the proposed pathway.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-072X
    Keywords: Lactobacillus plantarum ; Oxygen utilization ; Hydrogen peroxide accumulation ; NADH oxidase ; Pyruvate oxidase ; NADH peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two strains of Lactobacillus plantarum accumulated H2O2 when grown aerobically in a complex glucose based medium. The H2O2 accumulation did not occur immediately on exposure of the culture to O2 but was delayed for a time which, in the case of one strain, was dependent on the amount of inoculum used to seed the culture. The accumulation was always preceded by an increase in the rate of O2 utilization by the cultures. The latter coincided approximately with an increase in specific activity of NADH oxidase, pyruvate oxidase and NADH peroxidase. H2O2 was not a product of NADH oxidase in vitro but was formed in substantial quantities from O2 during oxidation of pyruvate. The three enzymes were induced by O2 and H2O2; the induction of NADH oxidase responded to lower levels of O2 (but not of H2O2) than the pyruvate oxidase or the NADH peroxidase.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 78 (1991), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A cosmid gene library of the genome of Lactococcus lactis subsp. lactis 712 was probed for the presence of 16S rRNA genes, using 32P 5′ end-labelled 16S rRNA fragments. Cosmid DNA from positive clones responsible for hybridisation was subcloned into a high copy number vector and a restriction map was constructed. The location of the 16S, 23S and 5S rRNA genes was determined on this map. Transcriptional promoter activity was identified upstream of the 5′ end of the 16S rRNA gene. By probing L. lactis 712 chromosomal DNA cut with a range of restriction endonucleases, with a conserved oligonucleotide to the 5′ end of the 16S rRNA gene, 6 copies of rRNA genes were identified.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 61 (1989), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A cosmid gene library of the genome of Lactococcus lactis subsp. lactis 712 has been constructed in the broad host range plasmid pLAFR1 in Escherichia coli LE392. Three lactococcal genes from the bank were identified by heterologous complementation of specific mutations in strains of E. coli. A cosmid clone encoding a putative lactose transport gene was identified by complementing an E. coli lacY mutant. The complemented clone supported the uptake of 14C lactose in transport assays. The DNA fragment responsible was subcloned and localised to a 1.28 kb fragment of the lactococcal chromosome.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 54 (1989), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design and performance of a thermoresistometer (TR-SC) are described. This instrument has a high heating (5.7°C/sec) and cooling rate and can be used to determine spore survival curves at temperatures up to UHT range. Spores can be heated in homogenized foods under constant monitoring of pH ± 0.001 u. pH) and stable temperatures (± 0.1°C). D, values can be determined to 0.008 min. This method takes less time and effort than by capillaries. The fit of Dt values to thermal death time curves (TDT) curves is better by the TR-SC than by the capillary method. Survival and TDT curves for Bacillus licheniformis Bacillus coagulans and Bacillus subtilis were determined in homogenized foods and in McIlvaine buffer at tempertures up to the UHT range.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Trends in Food Science and Technology 2 (1991), S. 67-68 
    ISSN: 0924-2244
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK and Malden, USA : Blackwell Science Inc
    Journal of food safety 25 (2005), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The objective of this investigation was to model the influence of the initial microbial concentration on the Escherichia coli resistance to chlorine. A suspension test was used to evaluate the antibacterial efficacy of chlorine. Chlorine doses ranged from 0.6 to 0.9 mL /L, from 0.3 to 0.7 m L /L, from 0.15 to 0.3 mL/L and from 0.1 to 0.2 mL /L when the initial microbial concentration was 1.2 × 108, 6 × 107, 6 × 106and 6 × 105 CFU/mL, respectively. In order to describe the survival curves of E. coli, a mathematical model based on a biphasic inactivation was used. Initial microbial concentration had a significant influence on the kinetics of inactivation and on the resistance of E. coli to chlorine. In order to obtain a good repeatability and reproducibility of the bacterial suspension test, the initial concentration of bacterial suspensions should be evaluated and standardized.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Social Science & Medicine 30 (1990), S. 829-835 
    ISSN: 0277-9536
    Keywords: Nottingham Health Profile ; cross-cultural comparisons ; validation ; weighting self-reported morbidity statements
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    International Journal of Food Microbiology 23 (1994), S. 209-213 
    ISSN: 0168-1605
    Keywords: Heat Resistance ; Peach puree ; Saccharomyces cerevisiae ; Yeast
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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