ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The type RIIβ regulatory subunit of protein kinase A is primarily expressed in adipose tissue and brain. Knockout mice suggest a role for RIIβ in regulating energy balance and adipose-tissue content, thus making it a potential target for therapeutic intervention in obesity. A truncated version of the RIα subunit has been used in a crystallographic study and was used here to design an analogous RIIβ construct. Despite substantial screening, conditions were not found for the crystallization of the truncated RIIβ subunit. However, limited proteolysis of the full-length RIIβ subunit identified boundaries of the `hinge' region and a fragment containing the two cAMP-binding domains which did crystallize. A recombinant version of the fragment was expressed and crystallized for X-ray diffraction studies. The crystals belong to the orthorhombic space group C222, with unit-cell parameters a = 91.6, b = 105.9, c = 85.8 Å, and diffracted to at least 2.3 Å.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900007150
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