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  • 1
    ISSN: 1432-0533
    Keywords: Immunohistochemistry ; Medulloepithelioma ; Cytoskeletal proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Four examples of human cerebral medulloepithelioma were studied immunohistochemically with a panel of antibodies and antisera to neuronal and glial proteins. The tumors, in addition to primitive medullary epithelium, contained areas of neuroblastic, ganglionic, astrocytic, ependymoblastic and ependymal differentiation, and, in one tumor, areas resembling polar spongioblastoma. Tumor cells throughout the primitive medullary epithelium displayed focal immunocreactivity for vimentin, glial fibrillary acidic (GFA) protein and for the neuron-associated class III β-tubulin isotype. Neuroblasts showed immunoreactivity for the class III β-tubulin isotype, microtubule-associated protein 2 and neuron-specific enolase. Immunoreactivity for neurofilament epitopes and synaptophysin was detected in areas of ganglionic differentiation and coincided with the demonstration of neurofibrils in Bielschowsky's silver impregnations. Vimentin was the only marker detected in ependymoblastic and ependymal rosettes or in areas of polar spongioblastoma, as well as in mesenchymal, cells. The results indicate that, even in very primitive neoplastic neuroepithelium, immunocytochemical evidence of early commitment of some of the cells to a neuronal or glial lineage can be demonstrated. The neuron-associated class III β-tubulin isotype appears to be one of the earliest markers indicative of neuronal differentiation in normal and neoplastic primitive neuroepithelium.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Development of primary cultured astrocytes can be influenced by interplay of steroids and growth factors (GFs). These mytogenic polypetides regulated the expression of cytoskeletal proteins depending on pretreatment with either dexamethasone (DEX) or estradiol (E2). We evaluated the contribution of each of these treatments to the expression of tissue transglutaminase (tTG), that is involved in cell differentiation. Treatment for 12 h with E2 and EGF as well as DEX and IGF-I significantly increased tTG expression in 15 DIV astrocyte cultures. A proliferative marker, such as cyclinD1 decreased as consequence of treatment increasing tTG expression. Taken together, these results indicate that steroid-GFs cross-talk plays a crucial role during astroglial cell maturation, and tTG may be indicated as mediator of the proliferative/differentiative response of primary cultured astrocytes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 125 (1996), S. 0 
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: The tectonic stress orientation is estimated in the lithosphere of northern Sicily, the southern Tyrrhenian sea and southern Calabria, and in the Wadati-Benioff zone below the Tyrrhenian, by inversion of fault-plane solutions of earthquakes covering a magnitude range from 2.5 to 7.1. Focal mechanisms of 97 earthquakes are taken from the literature, after a critical evaluation of their data quality. an average misfit of F= 13° indicates that the set of all shallow (〈50km) earthquakes is generated by a heterogeneous stress field. For three subsets, based on regional and magnitude separation, F was small enough (2.8°〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:0956540X:GJI857:les" location="les.gif"/〉F〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:0956540X:GJI857:les" location="les.gif"/〉 5.9°) to support the assumption of a homogeneous stress direction; for an additional subset, with F= 7.4°, such a condition is close to being fulfilled even though some heterogeneity appears to be present. The number of earthquakes in these subsets ranged from nine to 22, and the uncertainties of the principal stress directions were generally of the order of 20° at the 90 per cent confidence level. The earthquakes with M〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:0956540X:GJI857:ges" location="ges.gif"/〉 5 define a regional stress field with the greatest principal stress, σ1, dipping at a shallow angle to the south. In north-eastern Sicily and south-western Calabria the stress field estimated by earthquakes is extensional, with σ3 in a direction of WNW, and a near-vertical σ1, in agreement with the graben tectonics mapped geologically in this area. In western Sicily the σ1 direction is oriented WNW, but this result is judged less reliable than the others, based on the broader confidence limits of the solution and the average misfit of 7.4°. The earthquakes in the Wadati-Benioff zone define σ1 dipping at about 70° to the NW, subparallel to the zone, with σ2 horizontal and striking parallel to the zone.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 108 (1992), S. 0 
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: A statistical model of the microearthquake occurrence in the Messina area, assuming periodic transitions among several Generalized Poisson states, is proposed. Four states are defined, of different duration, cluster mean rate and earthquake interdependence level. Their sequence is cyclically repeated with annual period. The findings of this work can be seen as a generalization of the approach of Bender (1984) who considered transition phenomena of the seismic temporal pattern among distinct states of the Simple Poisson type. Further, the investigation aims at identifying possible factors leading to the observed periodic changes in the earthquake statistical parameters and to the main ‘deviations’ of experimental data from the model. The rainfall and regional tectonic stress patterns appear to be reasonable candidates for explaining the microearthquake periodicity and the most relevant anomaly of the observed seismic temporal pattern with respect to the model, respectively.
    Type of Medium: Electronic Resource
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