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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 49 (1999), S. 379-384 
    ISSN: 1612-1112
    Keywords: Capillary electrophoresis ; Nucleotides ; Cadmium complexation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The CE separation of twelve nucleotides (5′-mono-, di-, triphosphates of adenosine, guanosine, cytidine and uridine) was improved by adding cadmium ion to the ammonium citrate/citric acid buffer (pH 5, ionic strength 100 mM). Cadmium ion acts as a complexing agent for some nucleotides (ATP, CTP, GTP, UTP, GDP). In order to accelerate the separation, the electroosmotic flow was reversed by flushing the fused-silica capillary with 0.2 % aqueous solution of the polycationic surfactant hexadimethrine bromide. A good separation of the twelve nucleotides studied was then achieved on a dynamically coated capillary in less than 5 min by using an ammonium citrate/citric acid buffer (pH 5, ionic strength 100 mM) to which 2 mM cadmium ion has been added. High peak efficiencies were obtained (210 000 theoretical plates) and the resolution between two adjacent peaks was always greater than 1.5.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 48 (1998), S. 739-744 
    ISSN: 1612-1112
    Keywords: Capillary electrophoresis ; Nucleotides ; Inorganic cations ; Complexation with Mg and Ca
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The complexation equilibrium between nucleotides (ATP, ADP) and inorganic cations (Mg2+, Ca2+) has been studied by capillary electrophoresis. The equilibrium constant and the stoichiometry of nucleotide-inorganic cation complexes can be deduced from the dependence of the electrophoretic mobility of each nucleotide on the negative logarithm of the inorganic cation concentration. The experimental values of complexation constants determined by CE compare favorably with those in the literature. As expected, Mg2+ forms more stable complexes with ATP (logK=2.30 and 4.10 at pH 5 and 8, respectively) than with ADP (logK=1.92 and 3.15 at pH 5 and 8, respectively). In the pH range 4–8, the stoichiometry of ADP-Mg2+ and ADP-Ca2+ complexes is always 1∶1 whereas that of the complexes between these cations and ATP depends on pH-hence ATP-Mg2+ and ATP-Ca2+ complexes have 1∶1 stoichiometry at pH 5 and 1∶2 at pH 8.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 138-142 
    ISSN: 0935-6304
    Keywords: Capillary electrochromatography ; nucleosides ; short-end injection ; phenyl-bonded silica ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The influence of several experimental parameters (pH, ionic strength, organic modifier content of hydro-organic buffer) upon EOF, migration time, and retention factor has been studied in CEC with a phenyl-bonded silica column on a model mixture of five nucleosides. This paper illustrates the current interest in CEC as a method of resolving complex mixtures of neutral and ionic solutes and demonstrates the potential of the short-end injection method as a means of reducing analysis time.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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