Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Gonadal regulation of GABAA receptors in the different brain areas of the male Japanese quail (1991)
    Springer
    Experimental brain research 87 (1991), S. 634-640 
    Details
    ISSN: 1432-1106
    Keywords: Quantitative autoradiography ; GABAA receptors ; [3H] muscimol ; Saturation binding ; Gonadal steroids ; Male Japanese quail
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Quantitative autoradiography was used to investigate the distribution and effects of gonadal hormones on [3H] muscimol (specific GABAA receptor ligand) binding in the male Japanese quail brain. In gonadally intact Japanese quail brains, [3H] muscimol revealed a heterogeneous distribution with high GABAA receptor levels in the cerebellum pars granularis (656 fmol/mg wet weight of tissue) and in the pars molecularis (405 fmol/mg wet weight of tissue). Low receptor levels were found in the nucleus preopticus anterior and the nucleus lateralis of the hypothalamic regions (〈220 fmol/mg wet weight of tissue) as well as thalamic nuclei such as rotundus and pretectalis (220–261 fmol/ mg wet weight of tissue). Castration resulted in [3H] muscimol binding changes in both brain areas that contain steroid receptors and brain areas devoid of steroid receptors. In fact, castration led to high binding levels in the preopticus anterior nucleus and in the anterior neostriatum area, brain areas that are known to contain gonadal steroid receptors. Castration also elevated [3H] muscimol binding in the hyperstriatum ventrale and reduced binding levels in the paleostriatum augmentatum and the stratum griseum centrale area; all of these areas are known to be devoid of gonadal steroid receptors. At this point it was also important to know whether the gonadal steroid effect is due to alterations in the number of binding sites (Bmax) and/or the affinity binding state (KD). The saturation binding study, dealing with some of the areas described above in brains of male quails castrated or castrated and treated with testosterone or estradiol, demonstrated that the steroid replacement therapy was responsible for the changes of the Bmax. Diminishing Bmax values were displayed in the hypothalamic preoptic area and the hyperstriatum ventrale of the male quail treated with testosterone and estradiol while a reduced Bmax was obtained in the anterior neostriatum of the quail treated with the former steroid. Our findings suggest that these steroids might control some centrally mediated behavior activities through effects on the maximum number of GABAA binding sites in the male Japanese quail.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227088
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    ANF binding sites in the heart of the quail (Coturnix coturnix japonica)
    Amsterdam : Elsevier
    Peptides 14 (1993), S. 913-918 
    Details
    ISSN: 0196-9781
    Keywords: Atrial natriuretic factor ; Binding sites ; In vitro quantitative autoradiography ; Quail heart
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0196-9781(93)90066-P
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    GABA[A] receptor level changes in female hamster forebrain following in vivo estrogen progesterone and benzodiazepine treatment: a quantitative autoradiography analysis (1989)
    Springer
    Experimental brain research 75 (1989), S. 644-652 
    Details
    ISSN: 1432-1106
    Keywords: Female hamster ; Steroid hormone ; Benzodiazepines ; 3H-muscimol binding ; Quantitative autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The levels of gamma amino butyric acid (GABAA) receptors (i.e. 3H-Muscimol binding sites) were determined by quantitative neurotransmitter receptors autoradiography in ovariectomized (OVX), OVX-estradiolo (E), OVX-progesterone (P), OVX-E+P, diazepam (DZ) and DZ + Ro15-1788 treated female hamsters.The various hormonal treatments altered 3H muscimol binding in many brain areas, whereas DZ and DZ + Ro15-1788 had little influence on GABAA receptor levels at the onset of the blocked aggressive behavioral activity. For example, E, P and E+P all significantly increased 3H muscimol binding in medial preoptic area, ventromedial hypothalamic nuclei, and vertical diagonal bandmedial septal nucleus, whereas P treatment increased binding in the caudate-putamen and decreased it in reuniens nucleus of the thalamus. E and E+P treatments increased 3H muscimol binding in the corticomedial amygdala nucleus and hippocampus. Diazepam treatment decreased the GABAA receptor binding in the caudate-putamen and basolateral amygdala, while having no effect in the other brain regions where hormone treatment was effective. In vitro incubation of brain sections with micromolar concentrations of E or P did not change muscimol binding. These results suggest that ovarian steroids, and benzodiazepines to a lesser extent, modulate 3H muscimol binding but do so in different brain regions. The hormone effects on 3H muscimol binding in the critical reproductive centres at a time period that coincides with the onset of its behavioral effect is consistent with a genomic controlled activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249916
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Longer term progesterone treatment induces changes of GABAA receptor levels in forebrain sites in the female hamster: quantitative autoradiography study (1989)
    Springer
    Experimental brain research 77 (1989), S. 407-411 
    Details
    ISSN: 1432-1106
    Keywords: Receptor autoradiography ; Progesterone ; 3H muscimol binding ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Quantitative receptor autoradiography was applied to evaluate the effects of one and three injections of 1 mg progesterone (P) on 3H muscimol binding levels in the different forebrain areas of the female hamster. The overall effect of P resulted in substantial increases in 3H muscimol binding in brain areas containing gonadal steroid receptors: medial preoptic area and ventromedial hypothalamic nucleus as well as in bed nucleus stria terminalis and subiculum. Similarly, the caudate putamen, a region where gonadal steroid receptors are not abundant, also showed substantial increases of 3H muscimol binding receptor levels. Moreover, female hamsters treated with P for 3 days presented altered 3H muscimol binding levels in the amygdala and thalamic nucleus that were, in some cases, not produced by one dose of P. P treatment also decreased GABAA binding in two areas of the thalamus. These results are consistent with the proposal that P may alter GABAergic inhibitory activity via changes in the levels of GABAA receptors in certain forebrain areas in the female hamster, changes which may be linked to the mediation of anxiolytic effects and to the inhibition of aggressive behavior. These data also suggest that P treatment increases the binding of high affinity GABA receptors in some forebrain sites and may be responsible for maintenance of the anxiolytic effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274998
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...