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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 53 (1981), S. 2154-2156 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: Keywords NIDDM ; obesity ; fibroblasts ; glucose ; glucose transporter ; metabolism.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glucose transport and GLUT1 expression were studied in fibroblasts from 7 lean and 5 obese non-insulin-dependent diabetic (NIDDM) subjects with at least 2 NIDDM first-degree relatives and from 12 lean and 5 obese non-diabetic subjects with no family history of diabetes. The obese individuals also had a strong family history of obesity. Fibroblasts from all of the subjects exhibited no difference in insulin receptor binding, autophosphorylation, and kinase and hexokinase activity. At variance, basal 2-deoxyglucose (2-DG) uptake and 3H-cytochalasin B binding were 50 % increased in cells from individuals with NIDDM (p 〈 0.001) and/or obesity (p 〈 0.01) as compared to the lean non-diabetic subjects. Insulin-dependent (maximally stimulated – basal) 2-DG uptake and cytochalasin B binding were decreased three-fold in cells from the diabetic and/or obese subjects (p 〈 0.01). GLUT1 mRNA and total protein levels were comparable in fibroblasts from all the groups. However, basal GLUT1 cell-surface content was 50 % greater in fibroblasts from the NIDDM and/or obese subjects as compared to the lean non- diabetic individuals while insulin-dependent GLUT1 recruitment at the cell surface was diminished threefold. Increased basal GLUT1 content in the plasma membrane was also observed in skeletal muscle of 4 NIDDM and 3 non-diabetic obese individuals (p 〈 0.05 vs the lean non diabetic subjects). Basal 2-DG uptake in fibroblasts from diabetic/obese individuals and lean control subjects strongly correlated with the in vivo fasting plasma insulin concentration of the donor. A negative correlation was demonstrated between the magnitude of insulin-dependent glucose uptake by the fibroblasts and plasma insulin levels in vivo. We conclude that a primary abnormality in glucose transport and GLUT1 cell-surface content is present in fibroblasts from NIDDM and obese individuals. The abnormal GLUT1 content is also present in skeletal muscle plasma membranes from NIDDM and obese individuals. [Diabetologia (1997) 40: 421–429]
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Intensive care medicine 22 (1996), S. S85 
    ISSN: 1432-1238
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Conclusion Our results showed an high incidence of Long QT Interval in Intensive Care Patients (59.59%). The non-survivor rate of abnormal QTc group was not significant different comparing with normal QTc group of patients.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Several studies show that inflammatory components may contribute to atherosclerosis and increase the risk for myocardial infarction (MI). Interleukin-6 (IL-6) is a key pro-inflammatory and immune-modulatory cytokine of relevance for cardiovascular diseases. In this case-control study, 200 patients with MI and 257 healthy controls were genotyped for the polymorphism present in −174 promoter region of the IL-6 gene. Plasma concentrations of IL-6 and C-reactive protein (CRP) in a group of patients and controls were measured. The −174 C allele was associated with an increased risk of developing MI (OR = 2.886, c.i. = 1.801–4.624, P = 0.0001) in older patients, while no association was found in younger ones. The IL-6 plasma levels were higher in patients with MI carrying the CC genotype than in GG patients (CC carriers, IL-6 = 2.97 pg mL−1 vs. GG carriers = 1.81 pg mL−1, P = 0.016). A positive correlation of IL-6 levels with those of CRP in serum from patients with MI was also found. Data from this study suggest that the C allele of the promoter polymorphism in the IL-6 gene is a risk factor for MI in the elderly, and the production of the IL-6 is differentially affected by different genotypes of the IL-6 −174 promoter polymorphism.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background : Certain evidence suggests that Helicobacter pylori strains expressing genes for cytotoxin production show a higher sensitivity than non-cytotoxic organisms to eradication treatment. No data are available on the involvement of bacterium-related lesions in different therapeutic outcomes.Aims : (i) To investigate whether differences in eradication rates may be related to the different expression of virulent strains (cagA, vacA, iceA) in patients undergoing proton pump inhibitor-based triple therapy, and (ii) to evaluate whether therapeutic outcome may be affected by bacterium-induced gastric lesions.Methods : One hundred and ten H. pylori-positive subjects were enrolled. H. pylori was genotyped by polymerase chain reaction. Treatment consisted of lansoprazole–amoxicillin–clarithromycin, twice daily for 1 week. Eradication was checked by urea breath test.Results : The eradication rate was 70%, and the absence of cagA was associated with unsuccessful treatment. No difference between the groups with successful and unsuccessful eradication was found with regard to vacA and iceA. Lympho-epithelial lesions and fibrosis were associated with unsuccessful treatment.Conclusions : The present data confirm the importance of cagA (but not vacA and iceA) as a predictor of successful eradication. When fibrosis and lympho-epithelial lesions are present, therapy appears to be less effective. Therefore, these histological features may be involved in an unsuccessful therapeutic outcome.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The response to heat shock has been examined In two strains of the dimorphic pathogenic fungus Histoplasma capsulatum, which differ considerably in thermotolerance and pathogenicity. The gene for the 70 kD heat shock protein (hsp70) was isolated using a Drosophila hsp70 gene to screen a cosmid library of the DNA from the temperature-sensitive Downs strain (low level of thermotolerance for mice). Using the cloned gene as a probe, we have measured the transcription of the endogenous hsp70 gene at 25°C and in response to temperature shift to 34°, 37° and 40°C, temperatures that trigger the mycelial to yeast phase transition in this fungus. The gene is constitutively transcribed at low levels, both in the yeast and the mycelial stages. Synthesis of hsp70 mRNA was transiently increased 1 to 3 h after the temperature shifts. By Northern analysis, peak levels of transcription were shown to occur at 34°C in the Downs strain and at 37°C in the more pathogenic G222B strain. Our results are consistent with reports in which it has been shown that heat shock gene expression is part of temperature adaptation and probably developmental processes. The low levels of transcription of the hsp70 gene in the Downs strain at 37°C correlate with its greater temperature sensitivity and low level of virulence.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    International journal of earth sciences 71 (1982), S. 291-317 
    ISSN: 1437-3262
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Abstract The following main units have been recognized as Archaean basement rocks in central Brazil: a) High-grade granite-gneiss terrain composed predominantly by granodioritic and tonalitic gneisses; these rocks are cut by amphibolite dykes and present several nucleii locally surrounded by an array of Archaean greenstone belts, b) Greenstone belts presenting basal komatiitic ultramafic-mafic lavas with abundant spinifex textures, followed by basalts and chemical sediments such as cherts, limestones and graphitic pelites, that are often interbedded with acid and intermediate volcanics; metamorphism is within greenschist facies. Granulite mobile belt, including the granulitized mafic-ultramafic complexes of Barro Alto, Niquelândia and Brava. The Lower Proterozoic is represented by: a) metasedimentary sequences overlying the granite-greenstone terrains, with several tin bearing granite intrusions. b) Gabbro-anorthosite layered plutons and their associated mafic volcanics which are followed by acid and intermediate volcanics and ocean floor sediments. c) Mafic-ultramafic layered intrusions in the granulite terrain.
    Abstract: Resumo As seguintes unidades geológicas principais foram reconhecidas no embasamento da região central do Brasil: a) Terrenos granito-gnáissicos constituídos dominantemente por gnaisses de composição granodiorítica e tonalítica cortados por diques de anfibolito e mostrando vários núcleos graníticos localmente envolvidos por seqüências vulcano-sedimentares do tipo greenstone belt. b) Cinturões arqueanos de rochas verdes apresentando na base lavas komatiíticas com abundantes texturas tipo spinifex, seguidas de basaltos almofadados e de sedimentos químicos tais como chert, calcário e pelitos grafitosos, frequentemente intercalados com vulcânicas intermediárias e ácidas; o metamorfismo é de facies xisto verde. c) Cinturão móvel granulítico incluindo os complexos granulitizados de Barro Alto, Niquelândia e Cana Brava. Proterozóico Inferior é representado por: a) Seqüências sedimentares sobrepostas aos terrenos granito-greenstone, localmente intrudidas por granitos estaniferos; b) Plutões gabro-anortosíticos acamadados; c) Intrusões máfico-ultramáficas alojadas nos terrenos granulíticos. --Краткое содержание В центральной Бразил ии различают следующ ие основные единицы архейских отложений: а) высокоме таморфную гранито-гн ейсовую область с преимущественно гра нодиоритными и тонал итными гнейсами; эти породы пронизаны амфиболит ными жилами и образую т отдельные ядра, окруженные зонами архейского зе ленокаменного пояса, б) Зеленокаменный пояс, представляющий базальные коматиити ческие ультрамафиче ские до мафических лавы с частым проявле нием „спинофексных“ строений. За этими следуют базальты и химически е седименты, как кремн истый сланец, известняки и графитные пелиты, в ко торых часто встречаю т, как включения, кислые и средние вулканиты. Ме таморфизм отмечен в ф ации зеленого сланца; в) под вижный гранулитовый пояс, ко торый включает гранулитизированны е мафическо-ультрама фические комплексы Barro Alto, Niguelandia и Сапа Brava. В нижнем протерозое у становлено присутст вие следующих стратигра фических единиц: а) свиты метасе диментов, перекрытых гранито-зеленокамен ными сериями, и вмещающими некоторые интрузии г ранита, содержащие сурьму; б) прослои габб ро-анортозит-плутоно в с относящимися к ним ма фическими вулканитами, за котор ыми следуют кислые и с редние вулканиты и морски седименты; в) ма фическо-ультрамафич еские интрузии внутри гран улитовой фации.
    Notes: Zusammenfassung Im zentralen Brasilien wurden folgende Haupteinheiten als Archaischer Sockel erkannt: a) hochmetamorphes Granit-Gneis-Gebiet mit vorwiegend granodioritischen und tonalitischen Gneisen; diese Gesteine werden von amphibolitischen Gängen durchschlagen. Sie bilden einzelne Kerne, die von Zonen Archaischer Grünsteingürtel umgeben werden. b) Grünsteingürtel, die basale komatiitische ultramafitische bis mafischen Laven representieren mit häufig auftretenden “Spinifex”-Gefügen. Diesen folgen Basalte und chemische Sedimente wie Kieselschiefer, Kalke und graphitische Pelite, in die häufig saure und intermediäre Vulkanite eingeschaltet sind. Die Metamorphose liegt in der Grünschiefer-Fazies. c) Mobiler Granulit-Gürtel, der die granulitisierten mafisch-ultramafischen Komplexe von Barro Alto, Niquelândia und Cana Brava einschließt. Das untere Proterozoikum wird durch folgende Einheiten vertreten: a) Metasedimentfolgen, die die Granit-Grünstein-Serien überlagern und die einige zinnführende GranitIntrusionen aufweisen. b) Lagige Gabbro-Anorthosit-Plutone mit dazugehörenden mafischen Vulkaniten, die von sauren und intermediären Vulkaniten und ozeanischen Sedimenten gefolgt werden. c) Mafisch-ultramafische Intrusionen innerhalb des Granulit-Gebietes.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 188 (1982), S. 292-298 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tn7 insertions into the genome of F116L, a Pseudomonas aeruginosa generalized transducing phage, were isolated by repeated cycles of transducing phage, were of strains lysogenic for F116cts mutants with selection for trimethoprim resistance (Tp1). Two non-defective F116Lcts:Tn7 phage were characterized. They have reduced plaquing ability, produced non-lysogenic Tpr transductants, and have yielded a deletion mutant of the phage genome upon selection for plaque formation in single infection. F116L DNA is circularly permuted and terminally redundant. A circular restriction map of 61.7 kb has been defined, and a cleavage site common to many enzymes has been identified at coordinate 23.3 kb on the map. It is presumed that this site represents the sequence for the initiation of DNA encapsidation by a headful packaging mode. The Tn7 insertion targets and a 13.4 kb deletion define regions of the F116L genome non-essential for either vegetative growth or lysogenization. The restriction map of Tn7 has been determined for five enzymes. Non-lysogenic Tpr transuctants reveal a Tn7 insertion hot-spot in the P. aeruginosa genome.
    Type of Medium: Electronic Resource
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