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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 147 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: There is evidence that exopolysaccharides (EPS) contribute to the persistence of Pseudomonas aeruginosa in cystic fibrosis lung. However, the relationship between the chemical composition of EPS and the modulation of phagocytic cells is poorly understood. In order to evaluate the role of the chemical composition of EPS in macrophage behavior changes, we pretreated macrophages with characterized EPS and assessed P. aeruginosa phagocytosis and reactive oxygen intermediate (ROI) production. The results showed that alginate and neutral polysaccharides are involved in phagocytic impairment of P. aeruginosa. Moreover, alginates were able to prime macrophages for increased P. aeruginosa-induced macrophage oxidative burst as determined by chemiluminescence. In contrast, neutral polysaccharides are responsible for the decrease of ROI by a scavenging effect evaluated by the xanthine–xanthine oxidase system. This study showed that the content of P. aeruginosa EPS in alginate, but also in neutral polysaccharides, influences the behavior of strains towards phagocytosis and macrophage oxidative burst.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 10 (1991), S. 20-24 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Sixty-one strains ofCorynebacterium group D2 were examined for their ability to adhere to human uroepithelial cells and to agglutinate human and guinea-pig erythrocytes. Strains were isolated from samples of two origins: urine of bacteriuric patients and healthy skin of patients without urinary infection. In addition, the isolates were examined by scanning and transmission electron microscopy. Heavy adherence to the uroepithelial cells but weak hemagglutination were noted. No statistical association was demonstrated between the adherence and the origin of the strains (65.2 % of urinary isolates and 80 % of healthy skin isolates were adherent). On transmission electron microscopy, a close association was observed between adherent bacteria and cells on thin sections and only few strains were piliated with negative staining. These results do not support a role of adherence as a predictor of pathogenicity ofCorynebacterium group D2 which seems to act as an opportunistic pathogen in urinary tract infections.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 15 (1996), S. 841-842 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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