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  • 1
    Electronic Resource
    Electronic Resource
    Amplification of the tryptophan operon gene in Escherichia coli chromosome to increase l-tryptophan biosynthesis (1993)
    Springer
    Applied microbiology and biotechnology 40 (1993), S. 301-305 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Classical mutagenesis could desensitize the feedback inhibition of l-tryptophan (l-Trp) biosynthesis. Among the mutants, a5-fluorotryptophan-resistant strain, Escherichia coli EMS4-C25 produced 3 g/l of l-Trp within 18 h. The feedback-resistant l-Trp operon gene (trp) prepared from E. coli EMS4-C25 was inserted into pUC19 and pHSG576 to generate pTC701 and pTC576, respectively. When pHSG576 and pTC701 were introduced into E. coli EMS4-C25, chromosomal integration occured through homologous recombination. By using Souther hybridization, we demostrated that the integrated plasmids existed as multicopies. The strains with integrated foreign trp operon gene had higher activities of anthranilate synthase and Trp synthase than those found for the host strain and produced 9.2 g/l of l-Trp with 13% conversion yield from d-glucose. The integration and implification of the trp-operon-beraing plasmid avoided the plasmid instability and increased l-TRp production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00170384
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  • 2
    Electronic Resource
    Electronic Resource
    Role of porosity loss in limiting SO2 capture by calcium based sorbents (1989)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 988-994 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The extent of high temperature (900-1,300°C), short time (〈1 s) SO2 capture was found to be limited by temperature-dependent losses in the porosity of calcium based sorbents. At 970°C these porosity losses were caused by CO2-activated sintering. Sulfation of the sorbents either prevented or reduced the extent of porosity losses. Differences in SO2 capture between hydroxides from different commercial sources, and significantly lower levels of capture by calcium carbonates compared to hydroxides were attributed to differences in particle size and the degree of porosity loss.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690350612
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    Paper (German National Licenses)
    Fulltext
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