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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 9 (1990), S. 864-868 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Sequences derived from the endogenous plasmid ofChlamydia trachomatis and from the genes coding for ribosomal 16S RNA ofChlamydia psittaci were used as primers and oligonucleotide probes for detection of chlamydiae by the polymerase chain reaction. The endogenous plasmid primers generated specific amplified products of 517 bp with all knownChlamydia trachomatis serovars. No specific products ofChlamydia psittaci andChlamydia pneumoniae could be detected using these primers. With the rRNA primers specific amplified products of 208 bp were generated withChlamydia psittaci, Chlamydia trachomatis andChlamydia pneumoniae. No specific amplified products were detected with DNA isolated from a variety of microorganisms from the urogenital and the respiratory tract. Of 156 clinical specimens used for evaluation of the polymerase chain reaction, 26 were found to be positive forChlamydia trachomatis on culture. All 26 culture positive samples were also found to be positive forChalmydia trachomatis DNA by the polymerase chain reaction with both primer sets. Two culture negative samples were also found to be positive by this technique. The polymerase chain reaction thus seems to be a sensitive and reliable method for detection ofChlamydia trachomatis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 10 (1991), S. 714-727 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although it is now evident that human papillomaviruses (HPV) are strongly associated with cervical cancer, their etiological role in the oncogenesis of this disease is still unknown. However, HPV screening may identify women at risk of acquiring this disease. With the recent development of the polymerase chain reaction (PCR), it has become possible to detect small numbers of human papillomavirus genomes in clinical samples. The sensitivity and specificity of this technique, together with the possibility of performing the test on crude cervical scrapes, makes PCR the method of choice for screening. In this paper, data on the detection of human papillomavirus by PCR are presented and the applicability of this technique for the screening of human papillomavirus genotypes is evaluated. The question arises whether screening for diagnostic purposes must include all the human papillomavirus types associated with infections of the genital tract or only those which are strongly associated with cervical cancer (HPV 16 and HPV 18). It is proposed that an international council must be created that is responsible for standardised epidemiological screening strategies and follow-up programmes.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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