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  • 1
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 37 (1915), S. 2553-2560 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 148 (1987), S. 77-82 
    ISSN: 1432-072X
    Keywords: Protoplast formation ; Zymolase ; Novozyme ; β-Glucuromidase ; Intergeneric fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts from various strains of red-pigmented yeasts were generated at high frequency using improved procedures. The use of sulphur-containing amino acids and 2-deoxyglucose in growth media led to impaired cell wall synthesis and rendered cells very susceptible to treatment with mercapto-ethanol and various lytic enzymes. Use of individual lytic enzymes separately resulted in relatively low frequencies of protoplasts from most of the red yeasts examined, whilst use of β-glucoronidase, Novozyme and Zymolyase in series markedly increased stable protoplast formation. The latter effects were shown to be strain specific. The ability to generate large numbers of red yeast protoplasts prompted the attempt to examine intergeneric fusion between auxotrophs of a strain of Saccharomyces cerevisiae and Rhodotorula rubra. Putative hybrids were selected as variously-pigmented prototrophic colonies growing on minimal medium and stabilised by subculturing on the latter medium. Unusual cream, orange and yellow hybrid colonies were generated, composed of cells of varying morphologies (chains, multibudded). The majority of stable hybrids contained one nucleus, although several heterokaryons were also observed. Some hybrids possessed the phenotypes of both parents: fusant wcat41 grew as rapidly as the S. cerevisiae parent but also contained an inducible phenylalanine ammonia-lyase (PAL) which appeared to be more active than that of the Rhodotorula parent.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 28 (1992), S. 0 
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : Protection of ground water quality is of considerable importance to local, state, and federal governments. This study uses a 15-year mathematical programming model to evaluate the effectiveness of low-input agriculture, under alternative policy scenarios, as a strategy to protect ground water quality in Richmond County, Virginia. The analysis considers eight policy alternatives: cost-sharing for green manures, two restrictions on atrazine applications levels, chemical taxation, a restriction on potential chemical and nitrogen levels in ground water only and in surface and ground water, and two types of land retirement programs. The CREAMS and GLEAMS models were used to estimate nitrate and chemical leaching from the crop root zone. The economic model evaluates production practices, policy constraints, and water quality given a long-term profit maximizing objective. The results indicate that low-input agriculture alone may not be an effective ground water protection strategy. The policy impacts include partial adoption of low-input practices, land retirement, and the substitution of chemicals. Only mandatory land retirement policies reduced all chemical and nutrient loadings of ground water; however, they did not promote the use of low-input agricultural practices.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0922-338X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Public Health 12 (1991), S. 141-156 
    ISSN: 0163-7525
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Although 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE), a product of 15-lipoxygenase (15-LO), may be involved in mild to moderate asthma, little is known about its potential roles in severe asthma.Objectives This study was performed to evaluate 15(S)-HETE levels in bronchoalveolar lavage fluid (BALF) from severe asthmatics with and without airway eosinophils and from the control groups. In addition, 15-LO protein expression was examined in endobronchial biopsy, while its expression and activation were evaluated in BAL cells.Results While 15(S)-HETE levels in BALF were significantly higher in all severe asthmatics than normal subjects, severe asthmatics with airway eosinophils had the highest levels compared with mild, moderate asthmatics and normal subjects. 15(S)-HETE levels were associated with tissue eosinophil numbers, sub-basement membrane thickness and BALF tissue inhibitor of metalloproteinase-1 levels, and were accompanied by increased 15-LO expression in bronchial epithelium. In addition, activation of 15-LO was suggested by the increased proportion of 15-LO in the cytoplasmic membrane of alveolar macrophages from severe asthmatics.Conclusion The data suggest that severe asthmatics with persistent airway eosinophils manifest high levels of 15(S)-HETE in BALF, which may be associated with airway fibrosis. It is likely that 15-LO expression and activation by airway cells explain the increased 15(S)-HETE levels.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Sedimentology 16 (1971), S. 0 
    ISSN: 1365-3091
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Micritic envelopes are commonly believed to develop from the lime-mud infillings of circumferential perforations made in skeletal particles by algae. Because “algae” are usually taken to mean photosynthetic algae, the corollary has been proposed that micritic envelopes would not appear on carbonate skeletal particles originating in waters too deep for active growth of photosynthetic algae. Extensions of this “photosynthetic-algae-only” origin of micritic envelopes implies that: (1) the disappearance of envelopes with depth could mark a useful paleobathymetric datum; and (2) preservation of outlines of skeletal particles should be much better in “shallow”-water carbonates than in “deep”-water carbonates.We doubt the validity of the depth-limiting concept of the origin of micritic envelopes for two reasons: (1) petrographic study of a large number of thin sections thought to be representative of various paleo-depth levels does not indicate any systematic variations in the preservation of the outlines of skeletal particles; and (2) molluscan skeletal debris displaying micritic envelopes and effects of borings identical with those generally ascribed to the activities of photosynthetic algae have been found in an aragonite-cemented sandstone from the Atlantic shelf where geologic evidence indicates that the water at time of boring was too deep for active growth of photosynthetic algae.Accordingly, we re-emphasize the fact that organisms other than photosynthetic algae can bore into calcareous materials. In particular, fungi, bacteria, and heterotrophic algae (whose distribution does not depend on the penetration of light into seawater) are known to be active borers. Bacteria can be eliminated as producers of bores having diameters greater than approximately 10 μ. Fungi create boring structures with radiating or branching patterns or bores with nonuniform size. Some fungal bores appear to be indistinguishable from excavations known to have been bored by photosynthetic blue-green algae. At present we do not know how to distinguish the results of boring activity of photosynthetic algae from those of algae living het-erotrophically.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 72 (2001), S. 944-947 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: We have developed a novel diagnostic technique for weakly nonideal laser-produced plasma (LPP) plumes, based entirely on the continuum, and present the result for aluminum plasma. Due to significant self-absorption, the analysis is carried out in close coupling with plasma equilibrium calculations. We first invoke scaling relations linking the time- and position-resolved specific continuum intensity I, integrated over the spectral range of a streak camera, to the local temperature T and pressure p: T=STIα and p=SpIα+β. Inversion of the two-dimensional luminosity profile into the specific continuum intensity profile (three-dimensional) is carried out self-consistently, subject to the 16 independent measurements: target mass loss, energy deposited into the LPP plume and laser beam attenuation at fundamental and its harmonic frequencies, all at four different laser energies. An agreement to within 6.1% of the measurements overall has been achieved with α=0.45±0.03, β=1.0±0.03, ST=0.190±0.003 and Sp=340±5. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 77 (2000), S. 49-51 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The possibility of bonding clean diamond surfaces covalently in ultrahigh vacuum is investigated by molecular dynamics simulations based on a tight binding model. Our simulations predict that strong covalent bonding is possible for C(001)-2×1 surfaces. C(111)-2×1 surfaces will bond very weakly and debond already at moderate temperatures. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
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