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  • 1
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Pisum sativum L. ; Disease resistances ; Developmental traits ; Quantitative trait loci (QTL)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An F2 population of pea (Pisum sativum L.) consisting of 174 plants was analysed by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) techniques. Ascochyta pisi race C resistance, plant height, flowering earliness and number of nodes were measured in order to map the genes responsible for their variation. We have constructed a partial linkage map including 3 morphological character genes, 4 disease resistance genes, 56 RFLP loci, 4 microsatellite loci and 2 RAPD loci. Molecular markers linked to each resistance gene were found: Fusarium wilt (6 cM from Fw), powdery mildew (11 cM from er) and pea common Mosaic virus (15 cM from mo). QTLs (quantitative traits loci) for Ascochyta pisi race C resistance were mapped, with most of the variation explained by only three chromosomal regions. The QTL with the largest effect, on chromosome 4, was also mapped using a qualitative, Mendelian approach. Another QTL displayed a transgressive segregation, i.e. the parental line that was susceptible to Ascochyta blight had a resistance allele at this QTL. Analysis of correlations between developmental traits in terms of QTL effects and positions suggested a common genetic control of the number of nodes and earliness, and a loose relationship between these traits and height.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: drought ; cold ; heat ; screening techniques ; food legumes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Breeding for resistance to extremes of temperature and moisture in cool season food legumes is limited by the lack of adequate screening techniques. The success of each technique depends upon the representativeness and reproducibility of the type of stress created. Descriptions of successful techniques are presented for frost and terminal drought. Development of new screening tests designed to select for specific adaptive traits require a better knowledge of the mechanisms of resistance in these crops, especially to drought. Rooting depth, early vigor, reduced branching, and osmotic adjustment are discussed. Other mechanisms of resistance to drought, heat, freezing, or chilling have been proposed but need to be studied jointly by crop physiologists and plant breeders.
    Type of Medium: Electronic Resource
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