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  • 1
    Electronic Resource
    Electronic Resource
    The response of the uterine surface to ovarian hormones in the aged rat (1984)
    Springer
    Anatomy and embryology 169 (1984), S. 205-208 
    Details
    ISSN: 1432-0568
    Keywords: Rat uterus ; Epithelium ; Aging ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary By scanning electron microscopy uterine luminal epithelium of the rat was studied to determine whether aging alters ovarian hormone stimulated ultrastructural changes in that portion of the endometrial surface into which implantation takes place in the younger animal. Results show that in the aged rat this surface differentiates in response to ovarian hormones in a manner qualitatively similar to that which occurs in the young animal. Epithelial cells of ovariectomized rats, both young and aged, were polygonal in outline, flattened, or even somewhat concave, and had short microvilli. Following estrogen treatment cells of both groups were round or oval and bulged into the lumen. Cells of young rats were covered with long microvilli. Most cells of aged rats had microvilli of equal or greater length; a small number of epithelial cells had fewer and shorter microvilli. Cells of progesterone-treated young and aged animals both were covered with short microvilli and bore membrane protrusions. The protrusions varied in size, shape and numbers both within and between age groups. These findings suggest that differences in the surface ultrastructure of the aged uterus reflect age-related changes in hormone levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303150
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  • 2
    Electronic Resource
    Electronic Resource
    Epithelial ultrastructure during decidualization in rats (1981)
    Springer
    Anatomy and embryology 163 (1981), S. 215-222 
    Details
    ISSN: 1432-0568
    Keywords: Rat uterus ; Epithelium ; Ultrastructure ; Decidualization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Unilateral decidualization was induced in uteri of ovariectomized hormone-injected prepubertal and young adult rats. The antimesometrial luminal epithelia of both the decidualized and contralateral hormone-primed (non-decidual) control uteri were examined and compared by transmission and scanning electron microscopy. Epithelial cells of control uteri were columnar and they had many short microvilli on their luminal surfaces. Nuclei of these cells were round or oval and euchromatic, and other organelles were intact. In decidualized uteri luminal epithelial cells were flat and attenuated, were of greater average widths and possessed fewer microvilli. Some evidence of the degenerative changes which normally follow maximal decidual development in this region of the uterus could be seen within the flattened epithelial cells. The degenerative alterations were nuclear and cytoplasmic. Increase in lipid was observed in epithelial cells of decidualized uteri. This accumulation of intracellular lipid probably resulted from ingestion by the epithelial cells of intraluminally injected sesame oil, according to the protocol for stimulating decidualization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320677
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  • 3
    Electronic Resource
    Electronic Resource
    Recurrent glomerulonephritis in human renal homografts (1976)
    Springer
    Virchows Archiv 371 (1976), S. 5-14 
    Details
    ISSN: 1432-2307
    Keywords: Glomerulonephritis ; Renal ; Homograft
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Recurrent glomerulonephritis in kidneys transplanted to glomerulonephritic recipients is becoming more obvious. It has been suggested that the disease process which caused the original disease in the recipient is also operative in the transplanted tissue. This study compared the ultrastructure and immunofluorescence of the native kidneys of four patients with their respective sequential, transplant biopsies. In each case, subepithelial humps and IgG, characteristic of a complex type of nephritis, were observed in both the original diseased kidneys and in the transplants. This would indicate that the immunopathologic process which caused the original glomerulonephritis and led to transplantation is also operative in the transplanted tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433710
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  • 4
    Electronic Resource
    Electronic Resource
    Immunoelectron microscopic localization of galectin-3, an IgE binding protein, in human mast cells and basophils (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 242 (1995), S. 211-219 
    Details
    ISSN: 0003-276X
    Keywords: Epsilon binding protein ; Lectin ; Mast cell ; Basophil ; Immunogold ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Galectin-3 is an endogenous soluble lectin within the family called galectins that bind β-galactosides. Homologs of the protein isolated from different sources were previously designated as IgE-binding protein (∊BP), CBP35, CPB30, Mac-2, RL-29, RLL, L-29, and HL-29. All are now renamed galectin-3. This lectin is widely distributed in cells and tissues of mice, rats, dogs, hamsters, and humans.Light microscopic immunohistochemistry and ultrastructural immunogold labeling methods were used to determine the distribution of galectin-3 in human mast cells of several organs, in mast cells developed in vitro from human fetal liver cells, and in human peripheral blood basophils. Immunolabeling for the protein was observed in mast cells from all sources and in basophils. The lectin was detected in the nucleus and/or the cytoplasm. The nuclear labeling was over heterochromatin whereas euchromatin was unlabeled. Cytoplasmic labeling was concentrated over secretory granules. The intensity of staining generally was greater in mast cells of skin when compared with that of mast cells in other locations and with that of basophils. Studies have indicated that in mast cells galectin-3 may be involved in promoting their adhesion to basal laminae. In this study the localization of galectin-3 in the secretory granules of human mast cells and basophils suggests that these cells may release this lectin when activated to degranulate. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092420210
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  • 5
    Electronic Resource
    Electronic Resource
    Electron microscopic changes following canine cardiac allografts: The effects of irradiation (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 129 (1970), S. 169-175 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Orthotopic cardiac allotransplantation was performed on ten adult mongrel dogs. At the time of acute rejection (decline in QRS voltage to at least onehalf of the post-operative level) a cardiac biopsy was removed from the left ventricle of each dog and followed by 300 r of local x-irradiation on five consecutive days. After a full course of x-irradiation (1500r) to each dog a second biopsy was removed from the left ventricle. In this way, each dog acted as its own control in order to determine the effects of x-irradiation on cardiac transplants and the rejection phenomenon. The electron microscopic findings at the time of acute rejection involved primarily the myocytes and their cellular organelles in that the A, I, and Z bands were less distinct and there was significant enlargement and cytological distortion of the mitochondria, endoplasmic reticulum and T tubules. Endothelial hyperplasia of the smaller blood vessels was also more apparent in the pre-irradiation biopsies. Following irradiation the ultrastructural changes appeared to be reversed in six of the ten dogs; mitochondria and other organelles of the myocytes were more normal and the banding of the fibers was more distinct. Vascular alterations were less and the tissue approached normal cytological architecture. Pre- and post-irradiated cardiac tissues were not different in two animals and in two other animals the post-irradiated tissues appeared more abnormal than those showing acute rejection. Local irradiation as a method of immunosuppression in cardiac transplants is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001290204
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  • 6
    Electronic Resource
    Electronic Resource
    Fine structural observations on hemopoiesis in the chorioallantoic placenta of the marmoset (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 144 (1975), S. 9-37 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Hemopoietic sinuses in the chorionic trabeculae of marmoset placenta were examined electron microscopically at several stages of pregnancy. Tissues were prepared both by standard technique and following injection of exogenous peroxidase into the maternal circulation prior to autopsy, and subsequent cytochemical localization of its reaction product in the fetal placenta. Aside from their dilated size and cellular content, the sinuses resembled the allantoic capillaries of the peripheral umbilical circulation in this species; and, in early and midgestational placentas, sinusal endothelium, like that of capillaries, looked mesenchymal. Within the sinusal lumina, differentiating elements in the erythroid line were present in placentas at all stages. Such cells appeared to arise both by a process of proliferation of undifferentiated progenitors from the sinusal lining and by mitosis of pre-existing erythroid cells. Only the latter process was observed in the mature placenta near term. Granulopoiesis was not observed at any stage; on the other hand, cells in the thromboid line were identified in placentas believed to be near midgestation; and large intrasinusal phagocytes were present in both midgestational and late placentas. In the latter, they were seen to have endocytosed peroxidase, and, by tenuous, dendriform processes, to be related anatomically to large numbers of erythroid cells. Early in gestation, the developing erythroid elements were closely related by areas of contact, both to each other and to the delimiting endothelium of the sinuses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001440103
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