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  • 1
    Electronic Resource
    Electronic Resource
    HO-1 expression in primary cultured astroglial cells (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 85 (2003), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Heme oxygenase (HO)-1 catalyzes the rate-limiting step in heme degradation. The aim of the study was to determine HO-1 expression and distribution pattern in glial cells during differentiation and after exposure to glutamate (GLU) (100 μm). In untreated cells at 7 days in vitro (DIV) HO-1 expression was undetectable whereas increased at 14 and 21 DIV. Exposure to GLU for 24 h at 7, 14 and 21 DIV increased the expression of HO-1; this effect was prevented by preincubation with GYKI 52466. In untreated cells at 7 DIV, HO-1 fluorescence was weak; the protein appeared in the cytosol and the fluorescence intensity increased at 14 and 21 DIV. Compared with untreated cells, the addition of GLU increased the fluorescence and the enzyme translocated in the nucleus and nucleoli. These results demonstrate the presence of HO-1 in the glial cells and the translocation in the nuclear compartment of astrocytes during differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.85.s2.22_8.x
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  • 2
    Electronic Resource
    Electronic Resource
    Transglutaminase increases during astrocyte differentiation by interplay of growth factors and steroids (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 85 (2003), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Development of primary cultured astrocytes can be influenced by interplay of steroids and growth factors (GFs). These mytogenic polypetides regulated the expression of cytoskeletal proteins depending on pretreatment with either dexamethasone (DEX) or estradiol (E2). We evaluated the contribution of each of these treatments to the expression of tissue transglutaminase (tTG), that is involved in cell differentiation. Treatment for 12 h with E2 and EGF as well as DEX and IGF-I significantly increased tTG expression in 15 DIV astrocyte cultures. A proliferative marker, such as cyclinD1 decreased as consequence of treatment increasing tTG expression. Taken together, these results indicate that steroid-GFs cross-talk plays a crucial role during astroglial cell maturation, and tTG may be indicated as mediator of the proliferative/differentiative response of primary cultured astrocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.85.s2.22_7.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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