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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 615 (1991), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 253 (1975), S. 455-457 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Experiments were performed either with human heparinised platelet-rich plasma (PRP) adjusted to contain 470,000 platelets ul-1 (ref. 22) or with a suspension of washed human platelets (560,000 ul"1) suspended in Tyrode's solution containing 0.35 % bovine serum albumin and apyrase23. Platelet 5-HT ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A relationship between Borrelia burgdorferi and primary cutaneous B-cell lymphoma (PCBCL) has recently been confirmed following demonstration of the organism in lesional skin of patients with PCBCL. We report herein two cases of B. burgdorferi-associated PCBCL which strengthen this association by demonstrating the organism in cutaneous B-cell infiltrates present at sites in which PCBCL subsequently developed.All studies were performed on formalin-fixed paraffin-embedded tissues. These were examined by routine light microscopy and immunohistochemically by a standard streptavidin–biotin–complex technique. Genotypic studies were also undertaken using semi-nested polymerase chain reaction (PCR) for immunoglobulin heavy chain gene rearrangement, and nested PCR for B. burgdorferi flagellin gene. Both patients presented with erythematous skin lesions, biopsy of which showed dense perivascular infiltrates comprising small T-lymphocytes and collections of B-blasts. Primary cutaneous marginal zone lymphoma (MZL) developed subsequently in both cases at the same site. PCR for B. burgdorferi flagellin gene was positive in the perivascular lymphocytic infiltrates and the succeeding lymphomas in both patients.These results show that, at least in some instances, PCBCL arises from chronically stimulated lymphoid tissue acquired in the skin in response to B. burgdorferi infection. This may have significant therapeutic implications and warrant further studies on the extent of this association.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 313 (1985), S. 821-822 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] REPORTS by Knight and Scrutton1 and from our laboratory2 have shown that thrombin enhances the sensitivity to Ca2+ of the secretion of serotonin from permeabilized platelets. This effect was mimicked by adding a synthetic diacylglycerol, loleoyl-2-acetylglycerol1, and was closely associated with an ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The diet of the collembolan Gomphiocephalus hodgsoni was investigated by microscopic examination of the contents of 197 faecal pellets and 32 guts collected at Cape Geology, Victoria Land, Antarctica. Animals came from nine contrasting microhabitats, ranging from mineral soils to bryophyte cushions. Thirty-two different types of ingested material were recognised. Detritus, filamentous cyanobacteria, eukaryotic microalgae and fungal hyphae were the most frequent. There were marked contrasts between individuals collected from different microhabitats, which probably reflected food availability. There was some selectivity in that neither living moss tissue nor the macroscopic chlorophyte alga Prasiola crispa was encountered in faeces or guts, despite their being abundant in some microhabitats. A comparison of active and inactive animals showed the latter to have a significantly higher proportion of empty guts and of guts containing fat globules.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The diet of the collembolanGomphiocephalus hodgsoni was investigated by microscopic examination of the contents of 197 faecal pellets and 32 guts collected at Cape Geology, Victoria Land, Antarctica. Animals came from nine contrasting microhabitats, ranging from mineral soils to bryophyte cushions. Thirty-two different types of ingested material were recognised. Detritus, filamentous cyanobacteria, eukaryotic microalgae and fungal hyphae were the most frequent. There were marked contrasts between individuals collected from different microhabitats, which probably reflected food availability. There was some selectivity in that neither living moss tissue nor the macroscopic chlorophyte algaPrasiola crispa was encountered in faeces or guts, despite their being abundant in some microhabitats. A comparison of active and inactive animals showed the latter to have a significantly higher proportion of empty guts and of guts containing fat globules.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 18 (1999), S. 484-489 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The aim of this study was to make an evidence-based comparison of four commercial enzyme immunoassays (EIAs) (Serion Classics, Sigma Diagnostics, Cambridge Biotech and ICN Diagnostics) and an in-house enzyme immunoassay (EIA) in order to select the most appropriate screening assay for diagnosis of Lyme disease. Borrelia burgdorferi sensu stricto cultured in BSK-H medium was used to develop the in-house assay. Escherichia coli antigen (0.9 mg/ml) was included in the serum diluent to reduce non-specific background. Comparison of the number of tests needed to diagnose (i.e. to indicate a positive result) and the cost per positive diagnosis for the five assays was made using a panel of 176 Western blot-characterised sera. The Cambridge Biotech and Sigma assays had the highest sensitivity but poorer specificity, whereas the Serion and ICN assays had highest specificity but poorer sensitivity. The in-house assay had average sensitivity and specificity, the number of tests needed to diagnose being 2.32 compared to 1.92 for Serion, 2.17 for ICN, 2.5 for Sigma and 2.7 for Cambridge Biotech. In a diagnostic protocol that uses an EIA as screening test, with confirmation by Western blot, a good balance of sensitivity and specificity is essential. The in-house assay was the most cost-effective (lowest cost per positive diagnosis), and is probably the best option for specialist laboratories in Europe.
    Type of Medium: Electronic Resource
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