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  • 1
    Electronic Resource
    Electronic Resource
    Direct covalent mercuration of nucleotides and polynucleotides (1975)
    s.l. : American Chemical Society
    Biochemistry 14 (1975), S. 2447-2457 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00682a027
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of 3H-labeled supercoiled mitochondrial DNA from tobacco suspension culture cells (1980)
    Springer
    Molecular genetics and genomics 180 (1980), S. 351-355 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNA of tobacco supension culture cells labeled in vivo with 3H-thymidine was isolated from purified mitochondria. It has a density of 1.707±0.002 g/cm3 unique from both nuclear and chloroplast DNA. Approximately 25–45% of the mtDNA molecules from several preparations from suspension culture cells were isolated as supercoiled molecules from ethidium bromide-cesium chloride gradients. Electron microscopic examination of nicked supercoiled molecules revealed a heterogenous population of molecules ranging in size from 0.5 to 42 μm. The most abundant size class of 365 molecules measured was 9 μm. Restriction endonuclease digests of both supercoiled and open circular/linear molecules were essentially identical and yielded a minimum size estimate of 76 μm. The availability of large amounts of labeled covalently closed circular molecules will facilitate the resolution of the different size classes and allow, for the first time, a direct analysis of the relationships among the heterogenous population of DNA molecules found in plant mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425847
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    Paper (German National Licenses)
    Fulltext
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